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公开(公告)号：US06365104B1
公开(公告)日：2002-04-02
申请号：US09344990
申请日：1999-06-25
IPC分类号： G01N2100
CPC分类号： B01L3/5021 , G01N15/05 , G01N2015/055 , Y10T436/25 , Y10T436/25375
摘要： An improved system can be used to examine a centrifuged sample of anticoagulated whole blood for evidence of blood borne rare events such as: circulating cancer cells; malarial parasites; other hemato-parasites; bacteria; and the like; and can also be used in the measurement of hematocrit and hemoglobin, as well as white cell and platelet count values in the centrifuged blood sample. The system includes a transparent blood sample tube and an insert that is placed in the tube. The insert floats on the packed erythrocyte layer in the centrifuged blood sample, and expands all of the layers above the packed erythrocyte layer. The insert also forces any blood borne rare events to the periphery of the blood sample in the tube where such events can be detected through the tube wall. The bottom end of the tube is closed by a closure which includes an outer sheath and an inner plug that projects up into the tube bore to a degree necessary to elevate at least the upper portion of the packed erythrocyte layer into which the insert has settled sufficiently so as to be detectable above the cap, and preferably examinable microscopically after the blood sample has been centrifuged. The outer sheath of the tube closure includes an outer annular surface which is provided with sensible indicia. The indicia are operable to enable an instrument or a person examining the sample to rotate the tube, and to determine the circumferential location in the tube of any suspicious objects noted in the blood sample.
摘要翻译： 可以使用改进的系统来检查抗血清全血的离心样品，以证明血液传播的罕见事件，例如循环癌细胞; 疟疾寄生虫 其他血液寄生虫; 菌; 等等; 也可用于血细胞比容和血红蛋白的测量，以及离心血液样品中的白细胞和血小板计数值。 该系统包括透明血液样品管和放置在管中的插入物。 插入物漂浮在离心的血液样品中的包装的红细胞层上，并且使填充的红细胞层上方的所有层膨胀。 插入物还将任何血液传播的罕见事件强制在管中的血液样品的外围，其中可以通过管壁检测到这种事件。 管的底端由封闭件封闭，该封闭件包括外护套和内插塞，该内塞子向上突出到管孔中至少必须提升至少填充的红细胞层的上部，其中插入物已经充分沉积到其中 以便在帽上方可检测，并且优选在血液样品离心后在显微镜下进行检查。 管闭合件的外护套包括设有明显标记的外环形表面。 标记可操作以使仪器或检查样品的人旋转管，并确定管中在血液样本中记录的任何可疑物体的周向位置。
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112.发明授权Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication 失效使用空间局部化的靶分析物复制检测和定量样品中一种或多种核苷酸序列目标分析物
公开(公告)号：US6007990A
公开(公告)日：1999-12-28
申请号：US841267
申请日：1997-04-29
IPC分类号： C12N15/09 , C12N15/00 , C12Q1/68 , C12Q1/70 , C12R1/01 , C12R1/32 , C12R1/445 , C12R1/92 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q1/6834 , C12Q1/6846
摘要： A target analyte which may be found in a substance, such as a biological or environmental substance, is assayed by inoculating a media with a sample of the substance. The target analyte is a unique nucleotide sequence of the RNA or DNA of a suspect organism, or any nucleotide target, or a combination of nucleotide sequences thereof, which organism may be found in the substance. The media contains selected target analyte amplifiers which will result in the amplification of any target analytes which are present in the substance. The media may also contain one or more labeled analyte-specific materials (LASMs) which can migrate through the media. The nature of the media is such that it will support target analyte-copying but will not allow the target analyte to migrate extensively within the media. After the substance to be assayed is added to the media and any target analytes are amplified, the LASMs will migrate by diffusion to resultant target analyte units or colonies in the media, thereby creating intensely labeled localized areas in the media which can be visually or mechanically detected. Each of the intensely labeled areas in the media will be surrounded by low intensity label halos. The number of high intensity labeled sites in the media will be proportional to the concentration of target analytes in the substance.
摘要翻译： 通过用该物质的样品接种培养基来测定可以在物质如生物或环境物质中发现的目标分析物。 目标分析物是可疑生物体的RNA或DNA的唯一核苷酸序列，或任何核苷酸靶标或其核苷酸序列的组合，该物质可能存在有机体。 介质包含所选择的目标分析物放大器，这将导致物质中存在的任何目标分析物的扩增。 介质还可以包含一种或多种可以通过介质迁移的标记的分析物特异性材料（LASM）。 介质的性质使得它将支持目标分析物复制，但不允许目标分析物在介质中广泛迁移。 待分析物质加入到培养基中并扩增任何目标分析物后，LASM将通过扩散迁移到培养基中得到的目标分析物单位或菌落，从而在培养基中产生强烈标记的局部区域，可以在视觉上或机械上 检测到。 媒体中每个强烈标签的区域将被低强度标签光环包围。 介质中高强度标记位点的数量将与物质中目标分析物的浓度成比例。
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公开(公告)号：US5830639A
公开(公告)日：1998-11-03
申请号：US909209
申请日：1997-08-11
CPC分类号： B01L3/5021 , G01N33/491 , G02B21/34 , G01N2015/055 , Y10S435/81 , Y10S435/97 , Y10S436/805 , Y10S436/81
摘要： Centrifuged anticoagulated blood samples are analyzed under magnification in a centrifuge tube containing a layer-elongating insert, which tube is placed on a calibrated slide. The slide includes a slot in which the tube is placed. A calibrated scale is disposed adjacent to the slot for use in measuring various blood sample parameters, such as hematocrit, platelet count, and the like. Anemia and/or low platelet counts are indicative of potentially serious complications of malaria. Their detection will prompt a physician to consider the liklihood of serious illness due to malaria. The presence or absence of blood-borne parasites can also be determined using the procedures of this invention. Thus the device allows a blood sample to be analyzed for malarial parasites, and also allows measurement of hematicrit and platelet counts. The scale can be presented in a normal image when a simple lens magnification, such as a magnifying glass, is used to view the tube and slide; and can also be presented in mirror image when compound lens magnification, such as a microscope, is used to view the tube and slide.
摘要翻译： 将离心的抗凝血液样品在含有延伸层的离心管中放大分析，将该管置于校准的载玻片上。 滑动件包括一个狭槽，管子放在其中。 校准刻度尺邻近狭槽设置，用于测量各种血液样本参数，例如血细胞比容，血小板计数等。 贫血和/或低血小板计数表明疟疾潜在的严重并发症。 他们的检测将促使医生考虑疟疾引起的严重疾病的可能性。 血液寄生虫的存在或不存在也可以使用本发明的程序来确定。 因此，该装置允许血液样品被分析为疟疾寄生虫，并且还允许测量血浆和血小板计数。 当使用诸如放大镜的简单透镜放大倍率来观察管和滑动时，标尺可以呈现在正常图像中; 并且当复合透镜放大倍率（如显微镜）用于观察管和滑动时也可以以镜像呈现。
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114.发明授权Assembly for detecting blood-borne parasites and measuring blood sample parameters in a centrifuged sample of blood 失效用于检测血液寄生虫并测量离心的血液样本中的血液样本参数的组件
公开(公告)号：US5723285A
公开(公告)日：1998-03-03
申请号：US655465
申请日：1996-05-30
CPC分类号： B01L3/5021 , G01N33/491 , G02B21/34 , G01N2015/055 , Y10S435/81 , Y10S435/97 , Y10S436/805 , Y10S436/81
摘要： Centrifuged anticoagulated blood samples are analyzed under magnification in a centrifuge tube containing a layer-elongating insert, which tube is placed on a calibrated slide. The slide includes a slot in which the tube is placed. A calibrated scale is disposed adjacent to the slot for use in measuring various blood sample parameters, such as hematocrit, platelet count, and the like. Anemia and/or low platelet counts are indicative of potentially serious complications of malaria. Their detection will prompt a physician to consider the liklihood of serious illness due to malaria. The presence or absence of blood-borne parasites can also be determined using the procedures of this invention. Thus the device allows a blood sample to be analyzed for malarial parasites, and also allows measurement of hematicrit and platelet counts. The scale can be presented in a normal image when a simple lens magnification, such as a magnifying glass, is used to view the tube and slide; and can also be presented in mirror image when compound lens magnification, such as a microscope, is used to view the tube and slide.
摘要翻译： 将离心的抗凝血液样品在含有延伸层的离心管中放大分析，将该管置于校准的载玻片上。 滑动件包括一个狭槽，管子放在其中。 校准刻度尺邻近狭槽设置，用于测量各种血液样本参数，例如血细胞比容，血小板计数等。 贫血和/或低血小板计数表明疟疾潜在的严重并发症。 他们的检测将促使医生考虑疟疾引起的严重疾病的可能性。 血液寄生虫的存在或不存在也可以使用本发明的程序来确定。 因此，该装置允许血液样本被分析为疟疾寄生虫，并且还允许测量血浆和血小板计数。 当使用诸如放大镜的简单透镜放大倍率来观察管和滑动时，标尺可以呈现在正常图像中; 并且当复合透镜放大倍率（如显微镜）用于观察管和滑动时也可以以镜像呈现。
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公开(公告)号：US5393674A
公开(公告)日：1995-02-28
申请号：US025343
申请日：1993-03-08
IPC分类号： G01N1/10 , A61B5/15 , B01L3/14 , G01N1/14 , G01N1/36 , G01N33/15 , G01N33/48 , G01N33/49 , G01N1/18
CPC分类号： G01N33/491 , B01L3/50215 , Y10T436/25375 , Y10T436/2575
摘要： Constituent layers are harvested from a centrifuged multi-constituent material in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested, the use of an evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and are filled with an inert gas at low pressure. The floats are formed with a through bore into which cell bands to be harvested will settle during centrifugation. The cell bands are stabilized by a layer of a flowable material which settles onto the plasma layer during centrifugation and forms a pellicle thereon. The cell layers to be harvested are aspirated from the float bore by way of a hypodermic needle or cannula inserted into the tube and float bore.
摘要翻译： 在真空玻璃或含有浮子的透明塑料管中从离心的多组分材料中收获成分层。 当可能被污染的材料（例如血液）被测试时，使用真空管允许在没有技术人员暴露于血液的情况下进行测量。 管足够大以容纳约1ml血液，并在低压下填充惰性气体。 浮子形成有通孔，待收集的细胞带将在离心过程中沉降。 细胞带通过可流动的材料层稳定，其在离心过程中沉降到等离子体层上并在其上形成防护薄膜组件。 待收获的细胞层通过插入管中的皮下注射针或套管和浮子孔从浮子孔吸出。
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公开(公告)号：US5360719A
公开(公告)日：1994-11-01
申请号：US954440
申请日：1992-09-30
IPC分类号： G01N33/50 , G01N33/53 , G01N33/569 , C12Q1/04 , G01N33/49
CPC分类号： G01N33/5091 , G01N33/56972 , Y10S435/975 , Y10T436/25375
摘要： A patient's blood sample is incubated with an antigen and tested for lymphocyte response, i.e. an activation of lymphocytes and/or a conversion of lymphocytes to lymphoblasts, which indicates prior exposure of the patient to the antigen. A positive response indicates the presence of prior exposure to prior diseases or clinical conditions such as parasitic diseases, tuberculosis, salmonellosis, gonorrhea, fungal infections, rickettsial infections, Lyme disease or allergens. Whole blood from the patient is incubated with the antigen of the disease or condition for which the patient is being tested. After a suitable time, a fluorescent dye or colorant which has an affinity for a discriminant characteristic of the activated lymphocytes or lymphoblasts, such as: intracellular calcium; surface activation antigens such as transferrin receptor; HLA-Dr; Leu-23; and the like. The incubated blood is then drawn into a transparent tube containing a float which concentrates the buffy coat constituent layers upon centrifugation of the blood sample. The concentrated lymphocyte layer is then examined for fluorescence or coloration which is indicative of the presence of the activated lymphocytes or lymphoblasts, and their concentration. The fluorescence or coloration can be qualified and/or quantified by a reader instrument.
摘要翻译： 将患者的血液样品与抗原一起孵育并测试淋巴细胞反应，即淋巴细胞的激活和/或淋巴细胞向淋巴母细胞的转化，其指示患者事先暴露于抗原。 阳性反应表明先前接触先前疾病或临床病症如寄生虫病，结核病，沙门氏菌病，淋病，真菌感染，立克次体感染，莱姆病或过敏原。 将来自患者的全血与患者正在测试的疾病或病症的抗原一起温育。 在合适的时间之后，对活化的淋巴细胞或淋巴母细胞的鉴别特征具有亲和力的荧光染料或着色剂，例如：细胞内钙; 表面活化抗原如转铁蛋白受体; HLA-Dr; Leu-23; 等等。 然后将孵育的血液吸入含有浮子的透明管中，该浮子在血液样品离心时浓缩血沉棕黄层组成层。 然后检查浓缩的淋巴细胞层的荧光或着色，其指示活化的淋巴细胞或淋巴母细胞的存在及其浓度。 荧光或着色可由阅读器仪器进行鉴定和/或定量。
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公开(公告)号：US5342790A
公开(公告)日：1994-08-30
申请号：US969379
申请日：1992-10-30
申请人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
发明人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/536
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labeled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labeled antibodies will thus not interfere with the analysis.
摘要翻译： 通过在透明管中离心血液样品来诊断患者的健康，该管含有一组或多组颗粒，例如每组的不同密度的溶血细胞或塑料珠。 每组密度定义的颗粒携带对可能在被测试的血液样品中的补体抗原或抗体特异性的抗原或抗体，并且其表示患者的健康。 向血液样品中加入对所有结合的抗体/抗原对特异性的标记标签抗体，以在血液样品中形成标记抗体+抗原 - 抗体复合物（AAAC）。 离心后，根据颗粒的相应密度，络合的颗粒将沉淀在管中的不同区域中，并且颗粒层的标签发射程度可以使得要进行血液样品的定性或定量分析。 在离心步骤中，未结合的标记抗体将通过下降血细胞的洗涤作用从复合层中洗去。 因此，未结合的标记抗体不会干扰分析。
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公开(公告)号：US5251474A
公开(公告)日：1993-10-12
申请号：US822321
申请日：1992-01-16
CPC分类号： B01L3/50215 , G01N33/48 , G01N33/491 , G01N33/5002
摘要： Centrifuged material layer measurements are made in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested the use of the evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and are filled with an inert gas at low pressure. The floats are formed with a through passage into which the cell bands to be expanded will settle during centrifugation.
摘要翻译： 离心材料层的测量在包含浮子的真空玻璃或透明塑料管中进行。 当可能受污染的材料（例如血液）被测试时，使用真空管允许在没有技术人员暴露于血液的情况下进行测量。 管足够大以容纳约1ml血液，并在低压下填充惰性气体。 浮子形成有通孔，待离散的细胞带将在其中沉降。
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公开(公告)号：US4843869A
公开(公告)日：1989-07-04
申请号：US170771
申请日：1988-03-21
CPC分类号： G01N9/12 , B01L3/50215 , G01N33/491
摘要： A whole blood sample is placed in a tube such as a capillary tube, with a plastic float. The float is axially elongated and has a specific gravity which causes it to float in the packed red blood cells when the sample is centrifuged in the tube. The hemoglobin concentration of the packed red blood cells is measured by measuring the depth that the float sinks into the red cell layer, and then the hemoglobin concentration of the blood is calculated. The hemoglobin can be measured in this manner because virtually the only red cell component which contributes density to the red cells is the hemoglobin. The hemoglobin concentration of the whole blood is calculated by multiplying the mean corpuscular hemoglobin concentration of the packed red blood cells by the packed cell volume % (hematocrit) of the whole blood. All of the necessary calculations may be performed by a preprogramed microprocessor.
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公开(公告)号：US4779976A
公开(公告)日：1988-10-25
申请号：US91380
申请日：1987-08-31
IPC分类号： G01N15/05 , G01N33/487 , G01N33/16
CPC分类号： G01N15/05
摘要： Blood samples of animals are taken and analyzed for several different cell parameters. The analysis is made by centrifuging an anticoagulated blood sample in a capillary tube containing a float which physically elongates certain of the component cell layers in the sample. The instrument in which the parameter measurements are made is computerized and can be preset to take measurements of the blood of any one of several different types of animals whose respective blood cells have different packing coefficients.
摘要翻译： 拍摄动物的血液样品并分析几种不同的细胞参数。 通过在含有漂浮物的毛细管中离心抗凝血液样品进行分析，物理地延长样品中的某些组分细胞层。 进行参数测量的仪器是计算机化的，并且可以被预设为对各个血细胞具有不同包装系数的几种不同类型的动物中的任一种的血液进行测量。
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