公开(公告)号：US20160145593A1

公开(公告)日：2016-05-26

申请号：US14903194

申请日：2014-07-17

Applicant: ACADEMIA SINICA

Inventor： Meng-Chiao HO ,  Shih-Hsiung WU ,  Wan-Ling WU

IPC: C12N9/52 ,  C12P21/06 ,  C07K14/39 ,  C12N15/52

CPC classification number: C12N9/52 ,  C07K14/39 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/50 ,  C12N15/52 ,  C12P21/06 ,  C12Y304/21

Abstract: A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.

Abstract translation: 公开了一种编码台湾华ensis WR-220角蛋白酶的融合基因。 融合物包含：（a）编码位于融合基因N末端的蛋白质分泌信号肽的第一DNA序列; （b）编码与第一DNA序列连接的翻译框架的台湾华ensis WR-220角蛋白酶的抑制结构域的第二DNA序列; 和（c）编码与第二DNA序列连接的翻译框架的台湾华南WR-220角蛋白酶的催化结构域的第三DNA序列，其中融合基因是非天然存在的嵌合DNA。 还公开了一种制备M. taiwanensis WR-220角蛋白酶的催化结构域的方法，并使用了台湾华氏WR-220角蛋白酶。

公开(公告)号：US09224586B2

公开(公告)日：2015-12-29

申请号：US12976543

申请日：2010-12-22

Applicant: Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/0022 ,  H01J49/424 ,  H01J49/429

Abstract: Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.

Abstract translation: 公开了便携式质谱法的方法和装置。 该装置包括至少一个离子化分析物源，至少一个频率扫描子系统，至少一个检测器和可选的至少一个真空泵，并且是便携式的。 在一些实施方案中，装置包括多个电离分析物源和/或配置成获得大分析物的质谱，例如m / z比至少为105的分析物，或分子量至少为105的分析物 Da，以及小分子分析物的质谱。 在一些实施例中，所述方法包括使用上述便携式装置获得质谱。

公开(公告)号：US20150344513A9

公开(公告)日：2015-12-03

申请号：US14406074

申请日：2013-06-03

Applicant: Academia Sinica

Inventor： Shang-Cheng Hung ,  Cheng-Hsiu Chang

IPC: C07H15/04 ,  C07H1/00 ,  C07H23/00

CPC classification number: C07H15/04 ,  C07H1/00 ,  C07H3/06 ,  C07H15/18 ,  C07H15/203 ,  C07H23/00

Abstract: The present invention relates to methods for the synthesis of fondaparinux and intermediates thereto.

Abstract translation: 本发明涉及磺达肝素合成方法及其中间体。

公开(公告)号：US09187558B2

公开(公告)日：2015-11-17

申请号：US14381362

申请日：2013-03-01

Applicant: ACADEMIA SINICA

Inventor： Han-Chung Wu ,  Mei-Ying Liao ,  Cheng-Wei Lin

IPC: A61K39/395 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574 ,  A61K39/00

CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/574

Abstract: An isolated monoclonal antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment is characterized by: (a) having a specific binding affinity to epithelial cell adhesion molecule (EpCAM) comprising the amino acid sequence of SEQ ID NO: 1; (b) having a specific binding affinity to cancer cells expressing EpCAM said cancer cells being selected from the group consisting of oral cancer cells, nasopharyngeal cancer cells (NPC), colorectal cancer cells, and ovarian cancer cells; and (c) having no binding affinity to human umbilical vein endothelial cell (HUVEC) and normal nasal mucosal epithelia (NNM). Also disclosed is an isolated monoclonal antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope within the sequence of KPEGALQNNDGLYDPDCDE (SEQ ID NO: 63) located within the EGF-like domain II of epithelial cell adhesion molecule (EpCAM). Methods of using the same are also disclosed.

Abstract translation: 公开了分离的单克隆抗体或其抗原结合片段。 抗体或抗原结合片段的特征在于：（a）对包含SEQ ID NO：1的氨基酸序列的上皮细胞粘附分子（EpCAM）具有特异性结合亲和力; （b）对表达EpCAM的癌细胞具有特异性结合亲和力，所述癌细胞选自口腔癌细胞，鼻咽癌细胞（NPC），结肠直肠癌细胞和卵巢癌细胞; 和（c）对人脐静脉内皮细胞（HUVEC）和正常鼻粘膜上皮细胞（NNM）没有结合亲和力。 还公开了与位于上皮细胞粘附分子（EpCAM）的EGF样结构域II内的KPEGALQNNDGLYDPDCDE（SEQ ID NO：63）的序列内的表位具有特异性结合亲和力的分离的单克隆抗体或其抗原结合片段 ）。 还公开了使用该方法的方法。

公开(公告)号：US09186356B2

公开(公告)日：2015-11-17

申请号：US13343686

申请日：2012-01-04

Applicant: Che-Kun James Shen ,  Kuen-Jer Tsai

Inventor： Che-Kun James Shen ,  Kuen-Jer Tsai

IPC: A61K31/4436 ,  A61K31/132 ,  A61K31/138 ,  A61K31/513 ,  A61K31/55 ,  A61K31/7016 ,  A61K31/4433

CPC classification number: A61K31/4433 ,  A61K31/132 ,  A61K31/138 ,  A61K31/513 ,  A61K31/55 ,  A61K31/7016

Abstract: Methods for rescuing learning, memory and/or motor function deficits associated with frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U) are disclosed. The method comprises: a) administering to an animal having FTLD-U a therapeutically effective amount of an autophagy inducer; b) causing a decrease in the amount of ubiquitinated TDP-43 aggregation forms in the brain of the animal; and c) causing an improvement of the learning, memory capacities and/or motor function of the animal.

Abstract translation: 公开了拯救与泛素化包涵体（FTLD-U）的额颞叶退变相关的学习，记忆和/或运动功能缺陷的方法。 该方法包括：a）向具有FTLD-U的动物施用治疗有效量的自噬诱导物; b）导致动物脑中泛素化的TDP-43聚集形式的量减少; 和c）改善动物的学习，记忆能力和/或运动功能。

公开(公告)号：US20150315250A1

公开(公告)日：2015-11-05

申请号：US14702130

申请日：2015-05-01

Applicant: Academia Sinica

Inventor： Wolfgang Schmidt ,  Ping Lan ,  Louis Grillet

IPC: C07K14/415 ,  A23L1/304 ,  A61K36/31 ,  A23L1/212 ,  C12N15/82 ,  A61K33/26

CPC classification number: C07K14/415 ,  A23L7/101 ,  A23L19/00 ,  A23L33/105 ,  A23L33/16 ,  A23V2002/00 ,  A61K33/26 ,  A61K36/31 ,  C12N15/8243

Abstract: The present invention relates to a transgenic plant with increased trace element contents and a method for producing the same. In particular, the transgenic plant is incorporated by a polynucleotide encoding an iron-regulated protein 1 (IRP1/IMA1) or IRP1-like (IRL/IMA3) polypeptide, which facilitate uptake and circulation of the trace elements into the plant. Also provided is a method for treating trace element deficiency by administrating to a subject in need a composition comprising a transgenic plant as described or an edible tissue or part thereof.

Abstract translation: 本发明涉及具有增加的微量元素含量的转基因植物及其制备方法。 特别地，转基因植物通过编码铁调节蛋白1（IRP1 / IMA1）或IRP1样（IRL / IMA3）多肽的多核苷酸掺入，其促进微量元素吸收和循环进入植物。 还提供了通过向需要的受试者施用包含所述转基因植物或可食用组织或其部分的组合物来治疗微量元素缺乏症的方法。

公开(公告)号：US09096800B2

公开(公告)日：2015-08-04

申请号：US13751634

申请日：2013-01-28

Applicant: Frank H. Shu ,  Michael J. Cai ,  Fen-Tair Luo

Inventor： Frank H. Shu ,  Michael J. Cai ,  Fen-Tair Luo

IPC: C10B35/00 ,  C10L5/44 ,  C10L9/08 ,  C10B57/14 ,  C10B49/14 ,  C10B53/02

CPC classification number: C10B35/00 ,  C10B49/14 ,  C10B53/02 ,  C10B57/14 ,  C10L5/44 ,  C10L9/083 ,  Y02E50/10 ,  Y02E50/14 ,  Y02E50/15 ,  Y02E50/30

Abstract: A torrefaction system includes at least one pool containing a liquid heat transfer agent and a conveyor system. The heat transfer agent provides thermal contact with biomass fragments to heat the biomass fragments into biocoal. The conveyor system transports the biomass through the at least one pool in a first direction and transporting the biocoal in a second direction opposite to the first direction in the at least one pool. The heat transfer agent may be oil, paraffin, or molten salt. The conveyor system transports a continuous stream of the biomass fragments into the pools. The torrefaction apparatus further includes a gas collecting system that collects and separates condensable volatile organic compounds during the torrefaction process.

Abstract translation: 烘焙系统包括至少一个包含液体传热剂和输送系统的池。 传热剂提供与生物质碎片的热接触，将生物质碎片加热成生物炭。 输送机系统通过至少一个池沿着第一方向输送生物质并在至少一个池中沿与第一方向相反的第二方向运送生物炭。 传热剂可以是油，石蜡或熔盐。 输送系统将生物质碎片的连续流输送到池中。 烘焙装置还包括在烘焙过程中收集和分离可冷凝挥发性有机化合物的气体收集系统。

公开(公告)号：US09086416B2

公开(公告)日：2015-07-21

申请号：US13548181

申请日：2012-07-12

Applicant: Wen-Bin Yang

Inventor： Wen-Bin Yang

IPC: A61K31/70 ,  A61K31/7028 ,  A61K31/7042 ,  C07H15/00 ,  C07H15/04 ,  C07H17/00 ,  G01N33/58 ,  C07H15/26 ,  G01N33/531

CPC classification number: G01N33/58 ,  C07H15/26 ,  G01N33/531 ,  Y10T436/14

Abstract: Novel method and reagents for generating reversibly tagged saccharides, aldehydes, carboxyl acids, or orthoacetates useful in analytical and diagnostic applications are disclosed. Saccharides are coupled at the reducing end to tagging moieties comprising a reagent selected from a ortho-diaminobenzoic (DAB)-peptide, an aldo-imidazole or N-methylated aldo-imidazole, or an ortho-phenyldiamine (OPD) or substituted OPD. The tagged saccharide further comprising detectable or functional groups coupled to the tagging moiety are provided. Kits and reagents for chromatography and mass spectrometry are disclosed.

Abstract translation: 公开了用于生成可用于分析和诊断应用的可逆标记的糖，醛，羧酸或原乙酸盐的新方法和试剂。 糖在还原端耦合到包含选自邻二氨基苯甲酸（DAB）肽，醛 - 咪唑或N-甲基化的醛 - 咪唑或邻苯基二胺（OPD）或取代的OPD的试剂的标记部分。 提供了进一步包含与标记部分偶联的可检测或官能团的标记糖。 公开了用于色谱和质谱的试剂盒和试剂。

公开(公告)号：US09085768B2

公开(公告)日：2015-07-21

申请号：US13572541

申请日：2012-08-10

Applicant: Wan-Hsing Cheng ,  Ming-Hau Chiang ,  Ya-Huei Chen ,  Hwei-Ling Shen

Inventor： Wan-Hsing Cheng ,  Ming-Hau Chiang ,  Ya-Huei Chen ,  Hwei-Ling Shen

IPC: C12N15/82 ,  C12N15/113 ,  C07H21/04

CPC classification number: C12N15/113 ,  C12N15/8209 ,  C12N15/8237

Abstract: In this study, we used a wound-inducible promoter of the broccoli (Brassica oleracea var. italica) GLUCOSE INHIBITION of ROOT ELONGATION1 (GIR1) gene fused to β-glucuronidase (GUS, pBoGIR1::GUS) as a selectable marker. Transgenic broccoli plants expressing pBoGIR1::GUS appear blue in planta at wounded regions after GUS staining for 30 min. Similarly, the blue color is visible in transgenic Arabidopsis and rice plants expressing pBoGIR1::GUS at wounded areas after GUS staining for 2 h, indicating that this promoter is wound-inducible in both dicots and monocots. GUS staining is very rapid and the partial wounding in this study is a nondestructive method that does not affect further plant growth and development. Thus, pBoGIR1::GUS could serve as an effective substitute for antibiotic- and herbicide-resistance genes in the generation of genetically modified crops.

Abstract translation: 在这项研究中，我们使用了一个伤口诱导启动子的甘蓝（Brassica oleracea var。italica）GLUCOSE INHIBITION ROOT ELONGATION1（GIR1）基因融合到葡萄糖醛酸糖苷酶（GUS，pBoGIR1 :: GUS）作为选择标记。 表达pBoGIR1 :: GUS的转基因花椰菜植物在GUS染色30分钟后，在伤口区域的植物中呈蓝色。 类似地，在GUS染色2小时后，在受伤区域表达pBoGIR1 :: GUS的转基因拟南芥和水稻植物中，蓝色是可见的，表明该启动子在双子叶植物和单子叶植物中都是可诱导的。 GUS染色非常快，本研究中局部受伤是一种不影响植物生长发育的非破坏性方法。 因此，pBoGIR1 :: GUS可以作为转基因作物生产中抗生素和除草剂抗性基因的有效替代品。

公开(公告)号：US09067985B2

公开(公告)日：2015-06-30

申请号：US14041493

申请日：2013-09-30

Applicant: Academia Sinica

Inventor： Andrew H.-J. Wang ,  Min-Feng Hsu ,  Chii-Shen Yang ,  Hsu-Yuang Fu

IPC: C07K14/215

CPC classification number: C07K14/215 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/50

Abstract: An expression vector is disclosed, which comprises: a) a polynucleotide sequence encoding a bacteriorhodopsin or a mutant bacteriorhodopsin; b) a multiple cloning site; c) a T7 promoter, d) a polyhistidine tag; e) a first protease cleavage site; f) optionally a second protease cleavage site; and g) optionally a linker; wherein the mutant bacteriorhodopsin comprises the residue corresponding to Asn94 of SEQ ID NO: 1. Also disclosed is a fusion membrane protein expression system, which comprises: a) a polynucleotide sequence encoding a mutant Haloarcula marismortui bacteriorhodopsin/D94N (HmBRI/D94N) or a Haloquadratum walsbyi bacteriorhodopsin (HwBR); b) a target membrane protein; and c) a T7 promoter, operably linked to the mutant HmBRI/D94N or HwBR and the target membrane protein. Host cells comprising the expression vector or the fusion membrane protein expression system and methods of using the same are also disclosed.

Abstract translation: 公开了一种表达载体，其包括：a）编码细菌视紫红质或突变型细菌视紫红质的多核苷酸序列; b）多克隆位点; c）T7启动子，d）多组氨酸标签; e）第一个蛋白酶切割位点; f）任选的第二蛋白酶切割位点; 和g）任选的接头; 其中所述突变型细菌视紫红质包含对应于SEQ ID NO：1的Asn94的残基。还公开了融合膜蛋白表达系统，其包含：a）编码突变型Haloarcula marismortui细菌视紫红质/ D94N（HmBRI / D94N）或 Haloquadratum walsbyi细菌视紫红质（HwBR）; b）靶膜蛋白; 和c）与突变体HmBRI / D94N或HwBR和靶膜蛋白可操作地连接的T7启动子。 还公开了包含表达载体或融合膜蛋白表达系统的宿主细胞及其使用方法。