公开(公告)号：US20220298535A1

公开(公告)日：2022-09-22

申请号：US17750293

申请日：2022-05-20

申请人： Braskem S.A.

发明人： Paulo Moises Raduan ALEXANDRINO ,  Iuri Estrada GOUVEA ,  Veronica Leite QUEIROZ

IPC分类号： C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

摘要： The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20220259603A1

公开(公告)日：2022-08-18

申请号：US17610278

申请日：2020-05-21

申请人： Hyasynth Biologicals Inc.

发明人： Leanne BOURGEOIS ,  Alexander CAMPBELL ,  Elizabeth-Ann KRANJEC ,  Mindy MELGAR ,  Shoham MOOKERJEE ,  Sylvester PALYS ,  Alexandre THERRIEN ,  Curtis WALTON ,  Kevin WOO ,  Xiaohua ZHANG

IPC分类号： C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12N9/10 ,  C12P17/04 ,  C12P7/22 ,  C12P7/42

摘要： The present disclosure relates generally to methods and cell lines for the production of phytocannabinoids, phytocannabinoid precursors or intermediates, or phytocannabinoid analogue. Methods for transformation of host cells, such as yeast cells, are described. Cells may be transformed, for example, with a polynucleotide encoding a polyketide synthase (PKS) enzyme, a polynucleotide encoding an olivetolic acid cyclase (OAC) enzyme, and/or a polynucleotide encoding a prenyltransferase (PT) enzyme; and optionally a polynucleotide encoding an acyl-CoA synthase (Alk) enzyme; a polynucleotide encoding a fatty acyl CoA activating (CsAAE) enzyme; and/or a polynucleotide encoding a THCa synthase (OXC) enzyme.

公开(公告)号：US11382331B2

公开(公告)日：2022-07-12

申请号：US16449871

申请日：2019-06-24

申请人： MARRONE BIO INNOVATIONS, INC.

发明人： Ratnakar Asolkar ,  Marja Koivunen ,  Pamela G. Marrone ,  Ana Lucia Cordova-Kreylos ,  Huazhang Huang

IPC分类号： A01N63/20 ,  A01N43/00 ,  A01N43/16 ,  A01N43/76 ,  A01N43/86 ,  A01N43/90 ,  C12N1/20 ,  C12P17/06 ,  C12P17/14 ,  C12P17/16 ,  C07D309/14 ,  C07D407/06 ,  C07D413/04 ,  C07D413/06 ,  C07D263/32 ,  C07D263/34 ,  C07D493/10 ,  C07D498/14 ,  C07D513/04 ,  C12P17/04 ,  C12N1/00 ,  C12R1/01 ,  C12R1/00

摘要： A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.

公开(公告)号：US20220154240A1

公开(公告)日：2022-05-19

申请号：US17434418

申请日：2020-02-26

申请人： Guang An Mojia Biotechnology Co., Ltd.

发明人： Ansen CHIEW ,  Jinhuan SU ,  Congming ZENG ,  Tailong JIANG ,  Yan CHEN ,  Man Kit LAU

IPC分类号： C12P41/00 ,  C12P17/04

摘要： Disclosed is a method for resolving an optical isomer from a racemate by means of electrodialysis. Specifically, an electrodialysis technique is used in an enzymatic resolution process, mainly in the separation of products after enzymatic resolution. Taking a preparation process for D-pantolactone as an example, the key point is that D-pantoic acid and L-pantolactone are separated from an enzymatic resolution solution by means of an electrodialysis method, which replaces the existing organic solvent extraction method. The process method is simple and easy to operate, has a high yield of D-pantoic acid of a good purity, greatly reduces the usage amount of an organic solvent, reduces production costs and is environmentally friendly, such that the working environment of workers can be improved to a great extent, and the operation safety index is improved.

公开(公告)号：US11306317B2

公开(公告)日：2022-04-19

申请号：US16612413

申请日：2018-05-11

申请人： KIKKOMAN CORPORATION ,  NAGASAKI UNIVERSITY

发明人： Yasuko Araki ,  Yasutomo Shinohara ,  Kiyoshi Kita

IPC分类号： C12N15/52 ,  C12N1/14 ,  C12P7/24 ,  C12P7/26 ,  C12P17/04

摘要： The problem to be solved by the present invention is to provide a method of producing isoprenoids including ascofuranone, ilicicolin A, and ascochlorin and derivatives thereof in a high yield as compared to the conventional art, which method enables industrial-scale production of isoprenoids. The problem can be solved by a method of producing isoprenoids such as ascofuranone, ilicicolin A, and ascochlorin, including using a transformant obtained by transformation with biosynthetic genes for ascofuranone, ilicicolin A, or ascochlorin or a knockout organism for these genes to obtain isoprenoids such as ascofuranone, ilicicolin A, and ascochlorin.

公开(公告)号：US11293015B2

公开(公告)日：2022-04-05

申请号：US16951916

申请日：2020-11-18

申请人： Peking University ,  Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

发明人： Xiaoguang Lei ,  Jungui Dai ,  Lei Gao ,  Cong Su

IPC分类号： C12N9/50 ,  C12P7/26 ,  C12P17/04

摘要： The present invention provides a Diels-Alderase and use thereof, and belongs to the field of gene engineering technology. The Diels-Alderase is MaDA, and its amino acid sequence and gene sequence are represented by SEQ ID Nos. 1 and 2, respectively. The present invention also provides MaDA-1 and MaDA-2, both of which are homologous proteins of MaDA, and their amino acid sequences are represented by SEQ ID Nos. 10 and 12, respectively. The present invention has discovered that MaDA and its homologous proteins from Morus alba can stereospecifically synthesize natural products of endo configuration, and prepare D-A type natural products and their analogs in vitro using chalcones and dehydroprenyl-containing compounds as substrates, which helps to develop and utilize the medicinal value of such natural products, and also provides a possibility to synthesize other six-membered ring-containing important chemical precursors or natural products.

公开(公告)号：US11142748B2

公开(公告)日：2021-10-12

申请号：US16190937

申请日：2018-11-14

申请人： AMANO ENZYME INC.

发明人： Miho Nagaya ,  Akiko Sugita ,  Naoki Matsumoto ,  Masamichi Okada

IPC分类号： C12P7/42 ,  C12P19/12 ,  C12N9/04 ,  A23L29/00 ,  A23L15/00 ,  C12P7/58 ,  A21D8/04 ,  A23J1/00 ,  A23J3/04 ,  C12N15/52 ,  C12P7/44 ,  C12P17/04 ,  A23L5/00

摘要： A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics: (1) effect: oxidizing a saccharide to produce a saccharic acid; (2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and, (3) [Km value of glucose]/[Km value of maltose]≤1.

公开(公告)号：US20210292718A1

公开(公告)日：2021-09-23

申请号：US17200394

申请日：2021-03-12

申请人： California Institute of Technology

发明人： Kai Chen ,  Noah P. Dunham ,  Daniel J. Wackelin ,  Andrew Z. Zhou ,  Zhen Liu

IPC分类号： C12N9/02 ,  C12P17/04 ,  C12P17/16 ,  C12P7/62

摘要： The present disclosure provides cytochrome P450 variants useful for carrying out in vivo and in vitro carbene insertion reactions. Methods for preparing carbene insertion products including cyclopropenes, cyclopropanes, bicyclobutanes, substituted lactones, cyclized compounds, and substituted amines are also described.

公开(公告)号：US20210222138A1

公开(公告)日：2021-07-22

申请号：US15734887

申请日：2019-07-10

申请人： FIRMENICH SA

发明人： Michel Schalk ,  Letizia Rocci ,  Daniel Solis Escalante

IPC分类号： C12N9/16 ,  C12P7/04 ,  C12P7/18 ,  C12N9/04 ,  C12P17/04

摘要： Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.

公开(公告)号：US20210180095A1

公开(公告)日：2021-06-17

申请号：US16762336

申请日：2018-11-09

申请人： SHELL OIL COMPANY

发明人： Karl Alex Peter PAYNE ,  David LEYS ,  David Alexander PARKER ,  Stephen Andrew MARSHALL

IPC分类号： C12P17/04 ,  C12N9/88

摘要： Method for preparing a 2,5-furandicarboxylic acid (“FDCA”) comprising: contacting a polypeptide comprising an amino acid sequence that has greater than 34% sequence identity with an amino acid sequence set out in SEQ ID NO:1 or SEQ ID NO:2 with furoic acid in the presence of carbon dioxide; wherein the polypeptide has carboxylase and decarboxylase activity and comprises (i) the amino acid corresponding to H297 or a functional substitution thereof and (ii) at least one of (a) the amino acid corresponding to R305 or a functional substitution thereof; and (b) the amino acid corresponding to R332 or a functional substitution thereof; wherein the position is numbered relative to the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2.