公开(公告)号：US20220088560A1

公开(公告)日：2022-03-24

申请号：US17398865

申请日：2021-08-10

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: B01J19/00 ,  C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20220017953A1

公开(公告)日：2022-01-20

申请号：US17333792

申请日：2021-05-28

Applicant: Fluidigm Corporation

Inventor： Michael L. Phelan ,  Christopher J. Kubu ,  Brian Fowler ,  Gang Sun ,  Nikita Patel ,  Naveen Ramalingam

IPC: C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/70

Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.

公开(公告)号：US20210268508A1

公开(公告)日：2021-09-02

申请号：US17181966

申请日：2021-02-22

Applicant: Fluidigm Corporation

Inventor： Michael L. Phelan ,  Christopher J. Kubu ,  Brian Fowler ,  Gang Sun ,  Nikita Patel ,  Naveen Ramalingam

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/686

Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.

公开(公告)号：US20200264205A1

公开(公告)日：2020-08-20

申请号：US16752073

申请日：2020-01-24

Applicant: Fluidigm Corporation

Inventor： Jason A. A. West ,  Brian Fowler

IPC: G01N35/08 ,  G01N33/50 ,  G01N33/569 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/04 ,  C12M1/00

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：US20200240895A1

公开(公告)日：2020-07-30

申请号：US16844373

申请日：2020-04-09

Applicant: Fluidigm Corporation

Inventor： Dmitry R. Bandura ,  Vladimir I. Baranov ,  Scott D. Tanner

IPC: G01N15/14 ,  H01J49/04 ,  H01J49/00

Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.

公开(公告)号：US20190352697A1

公开(公告)日：2019-11-21

申请号：US16225349

申请日：2018-12-19

Applicant: Fluidigm Corporation

Inventor： Robert C. Jones

IPC: C12Q1/686 ,  G01N33/532 ,  C12Q1/682

Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.

公开(公告)号：US10322411B2

公开(公告)日：2019-06-18

申请号：US15150062

申请日：2016-05-09

Applicant: Fluidigm Corporation

Inventor： Naga Gopi Devaraju ,  Marc A. Unger

IPC: F16K11/20 ,  B01L3/00 ,  F17D3/00 ,  G01N35/00

Abstract: A microfluidic system includes a substrate, a set of input ports coupled to the substrate, and a set of output ports coupled to the substrate. The microfluidic system also includes a microfluidic processing system coupled to the substrate and including a plurality of processing sites. The microfluidic processing system is coupled to the set of input ports and the set of output ports. The microfluidic system further includes one or more microfluidic logic devices coupled to the substrate and operable to control at least a portion of the microfluidic processing system.

公开(公告)号：US10226770B2

公开(公告)日：2019-03-12

申请号：US14864491

申请日：2015-09-24

Applicant: Fluidigm Corporation

Inventor： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

IPC: C12Q3/00 ,  B01L7/00 ,  B01L3/00 ,  C12P19/34 ,  G01N21/64 ,  G01N21/76

Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.

公开(公告)号：US10190163B2

公开(公告)日：2019-01-29

申请号：US15055252

申请日：2016-02-26

Applicant: Fluidigm Corporation

Inventor： Carolyn G. Conant ,  Tze Howe Charn ,  Jason A. A. West ,  Xiaohui Wang

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6844 ,  B01L3/00

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：US20180306683A1

公开(公告)日：2018-10-25

申请号：US15925600

申请日：2018-03-19

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

IPC: G01N1/28 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/14

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.