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    Light-Switchable Gene Expression System and the Methods for Controlling Gene Expression in Prokaryotic Bacterium
    132.
    发明申请
    Light-Switchable Gene Expression System and the Methods for Controlling Gene Expression in Prokaryotic Bacterium 审中-公开
    光可切换基因表达系统和控制原核细菌基因表达的方法

    公开(公告)号：US20160040173A1

    公开(公告)日：2016-02-11

    申请号：US14419106

    申请日：2013-08-01

    Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

    Inventor： YI YANG ,  XIANJUN CHEN ,  ZHENGCAI MA ,  RENMEI LIU

    IPC: C12N15/63 ,  C12N15/70 ,  C12N13/00

    CPC classification number: C12N15/635 ,  C12N9/0051 ,  C12N13/00 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12P21/02

    Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.

    Abstract translation: 提供了一种原核细菌的光学控制基因表达系统，其包含：a）感光重组转录因子编码基因，所述感光重组转录因子是包含第一多肽作为DNA结合结构域的第一种融合蛋白，第二多肽作为感光结构域 ; b）包含启动子或启动子 - 反应元件或含有至少一个被第一多肽识别/结合的反应元件和待转录的核酸序列的反应元件的靶转录单元。 还提供了包含所述光学控制的基因表达系统的原核表达载体和通过使用光学控制的基因表达系统调节原核宿主细胞中的基因表达的方法。 还提供了含有光学控制的基因表达系统的不同成分的试剂盒。 原核细菌的光学控制基因表达系统具有快速，有效和强大的诱导，比其他诱导物更安全，毒性很小或无毒，可以在空间和时间上控制基因表达，并且可以调节原核细菌的许多生命过程 。

    CALIBRATION METHOD FOR THE BRITTLE FRACTURE ASSESSMENT PARAMETERS FOR MATERIALS BASED ON THE BEREMIN MODEL
    135.
    发明申请
    CALIBRATION METHOD FOR THE BRITTLE FRACTURE ASSESSMENT PARAMETERS FOR MATERIALS BASED ON THE BEREMIN MODEL 有权
    基于BEREMIN模型的材料脆性断裂参数的校准方法

    公开(公告)号：US20140372060A1

    公开(公告)日：2014-12-18

    申请号：US14365273

    申请日：2012-11-27

    Applicant: East China University of Science and Technology

    Inventor： Fuzhen Xuan ,  Yupeng Cao ,  Hu Hui ,  Penning Li ,  Guozhen Wang

    IPC: G01N3/62 ,  G01N3/40 ,  G06F17/50

    CPC classification number: G01N3/62 ,  G01N3/40 ,  G01N2203/0067 ,  G01N2203/021 ,  G01N2203/0218 ,  G06F17/5018

    Abstract: A calibration method for brittle fracture assessment parameters for pressure vessel materials based on the Beremin model includes selecting at least two types of specimens of different constraints, and calculating the fracture toughness values K0 corresponding to 63.2% failure probability for each type of specimens at a same calibration temperature by using the respective fracture toughness data. The method proceeds by obtaining the stress-strain curve of the material at the calibration temperature, generating finite element models for each type of specimens, and calculating the maximum principal stress and element volume of every element at K=K0 in each model. A series of values of m are assumed to compute a group of σu values for each type of specimens, and then m˜σu curves are plotted for each type of specimens. Brittle fracture assessment parameters are then determined for the material according to the coordinates of the intersection of the m˜σu curves.

    Abstract translation: 基于Beremin模型的压力容器材料的脆性断裂评估参数的校准方法包括选择不同约束条件的至少两种类型的试样，并计算相同于每种类型试样的63.2％失效概率的断裂韧性值K0 通过使用相应的断裂韧度数据来校准温度。 该方法通过在校准温度下获得材料的应力 - 应变曲线，为每种类型的样品生成有限元模型，并计算每个模型中K = K0处的每个元素的最大主应力和元素体积。 假设m的一系列值计算每种类型样本的一组＆sgr u值，然后对于每种类型的样本绘制m〜＆sgr; u曲线。 然后根据m〜＆ugr曲线的交点坐标确定材料的脆性断裂评估参数。

    Nicotinamide Adenine Dinucleotide Gene Encoding Fluorescent Probe, Preparation Method Therefor and Application Thereof
    136.
    发明申请
    Nicotinamide Adenine Dinucleotide Gene Encoding Fluorescent Probe, Preparation Method Therefor and Application Thereof 有权
    烟酰胺腺嘌呤二核苷酸基因编码荧光探针及其制备方法及应用

    公开(公告)号：US20140329718A1

    公开(公告)日：2014-11-06

    申请号：US14347575

    申请日：2012-09-26

    Applicant: East China University of Science and Technology

    Inventor： Yi Yang ,  Jing Jin ,  Yuzheng Zhao ,  Qingxun Hu

    IPC: G01N33/573 ,  G01N33/58 ,  G01N33/50

    CPC classification number: G01N33/5735 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00 ,  C07K2319/60 ,  G01N33/5011 ,  G01N33/582 ,  G01N2500/02 ,  G01N2800/7028

    Abstract: The invention relates to a genetically encoded fluorescent sensor for nicotinamide adenine dinucleotide, as well as methods of preparation and uses thereof. In one aspect, this invention relates to a sensor for detecting nicotinamide adenine dinucleotide, particularly, a recombinant fluorescent fusion protein sensor for detecting nicotinamide adenine dinucleotide. In one specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting reduced nicotinamide adenine dinucleotide (NADH); in another specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting oxidized nicotinamide adenine dinucleotide (NAD+); in yet another aspect, the invention relates to a recombinant fluorescent fusion protein sensor for detecting the ratio of reduced to oxidized nicotinamide adenine dinucleotide. This invention also relates to the method of preparing the sensors, and uses of the sensors in detecting NADH, NAD+, NADH/NAD+ ratio, screening drugs and measuring NADH metabolism.

    Abstract translation: 本发明涉及用于烟酰胺腺嘌呤二核苷酸的遗传编码荧光传感器，以及其制备方法和用途。 一方面，本发明涉及用于检测烟酰胺腺嘌呤二核苷酸的传感器，特别是用于检测烟酰胺腺嘌呤二核苷酸的重组荧光融合蛋白传感器。 在一个具体方面，本发明涉及用于检测还原型烟酰胺腺嘌呤二核苷酸（NADH）的重组荧光融合蛋白传感器。 在另一具体方面，本发明涉及用于检测氧化烟酰胺腺嘌呤二核苷酸（NAD +）的重组荧光融合蛋白传感器。 在另一方面，本发明涉及用于检测还原氧化烟酰胺腺嘌呤二核苷酸比例的重组荧光融合蛋白传感器。 本发明还涉及传感器的制备方法，以及传感器检测NADH，NAD +，NADH / NAD +比例，筛选药物和测定NADH代谢的用途。

    PROCESS FOR PREPARING AMORPHOUS POLYESTER HOT MELT ADHESIVE WITH LOW MELTING POINT
    137.
    发明申请
    PROCESS FOR PREPARING AMORPHOUS POLYESTER HOT MELT ADHESIVE WITH LOW MELTING POINT 审中-公开
    用于制备具有低熔点的无定形聚酯热熔胶的方法

    公开(公告)号：US20140256875A1

    公开(公告)日：2014-09-11

    申请号：US14355534

    申请日：2013-05-31

    Applicant: Shanghai TianYang Hot Melt Adhesive Co., Ltd. ,  Kunhan TianYang hot Melt Adhesive Co., Ltd. ,  East China University of Science and Technology

    Inventor： Zhelong Li ,  Wanyu Zhu ,  Zhiyan Ma ,  Zuoxiang Zeng ,  Tao Han

    IPC: C09J167/02

    CPC classification number: C09J167/02 ,  C08G63/183 ,  C08G63/78

    Abstract: A method of preparing an amorphous, low-melting-point polyester hot-melt adhesive is disclosed. The method includes the steps of: 1) esterifying terephthalic acid, isophthalic acid and an aliphatic dicarboxylic acid with ethylene glycol and a C3-C6 dial in a presence of an esterification catalyst; 2) adding a stabilizer, a polycondensation catalyst and an antioxidant to a product from step 1) and lowering the pressure to perform a polycondensation reaction which results in a polyester having a melting point of lower than 120° C. The polyester hot-melt adhesive of the invention assumes a transparent state with no crystallization both in normal-temperature and high-temperature conditions and has a high flowability and an excellent wettability in low-temperature applications.

    Abstract translation: 公开了一种制备无定形低熔点聚酯热熔粘合剂的方法。 该方法包括以下步骤：1）在酯化催化剂存在下，使对苯二甲酸，间苯二甲酸和脂族二羧酸与乙二醇和C3-C6表面酯化; 2）向步骤1）的产物中加入稳定剂，缩聚催化剂和抗氧化剂，降低压力进行缩聚反应，得到熔点低于120℃的聚酯。聚酯热熔胶 在常温和高温条件下呈现无结晶的透明状态，并且在低温应用中具有高流动性和优异的润湿性。

    Methods for detecting plasticizers
    138.
    发明授权
    Methods for detecting plasticizers 有权
    检测增塑剂的方法

    公开(公告)号：US08822384B2

    公开(公告)日：2014-09-02

    申请号：US13814610

    申请日：2011-09-30

    Applicant: Bangce Ye ,  Min Zhang

    Inventor： Bangce Ye ,  Min Zhang

    IPC: G01N31/22 ,  B82Y30/00 ,  B82Y15/00

    CPC classification number: G01N31/22 ,  B82Y15/00 ,  B82Y30/00 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/92

    Abstract: Nanoparticles having one or more attached sensing moieties including uridine 5′-triphosphate (UTP) and deoxythymidine 5′-triphosphate (dTTP), are disclosed herein. These nanoparticles can, for example, be used for detection of plasticizers, such as phthalates, in the sample. Methods, kits and apparatuses using these nanoparticles for detecting plasticizers in a sample are also disclosed herein.

    Abstract translation: 具有包含尿苷5'-三磷酸（UTP）和脱氧胸苷5'-三磷酸（dTTP）的一个或多个连接的感测部分的纳米颗粒在本文中公开。 例如，这些纳米颗粒可用于检测样品中的增塑剂，例如邻苯二甲酸酯。 本文还公开了使用这些纳米颗粒检测样品中增塑剂的方法，试剂盒和装置。

    MONOMERS, OLIGOMERIC COMPLEXES, COORDINATION POLYMERS, AND METHODS FOR THEIR PREPARATION AND USE
    140.
    发明申请
    MONOMERS, OLIGOMERIC COMPLEXES, COORDINATION POLYMERS, AND METHODS FOR THEIR PREPARATION AND USE 审中-公开
    单体，低分子复合物，协调聚合物及其制备和使用方法

    公开(公告)号：US20140121349A1

    公开(公告)日：2014-05-01

    申请号：US14149611

    申请日：2014-01-07

    Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

    Inventor： Yongshu XIE

    IPC: C08G79/00 ,  C07F3/06 ,  C07D213/36

    CPC classification number: C08G79/00 ,  C07D213/36 ,  C07D213/74 ,  C07F3/003 ,  C07F3/06

    Abstract: A hydrogel composition is formed by conveying separate first and second liquid components subject to a selectively applied application pressure P(A) into an outlet path for mixing and discharge. A liquid flushing agent is automatically conveyed into the outlet path subject to a substantially constantly applied purge pressure P(P) when the application of P(A) is interrupted, to continuously flush residual hydrogel composition from the outlet path.

    Abstract translation: 通过将经过选择性施加的施加压力P（A）的分离的第一和第二液体组分输送到用于混合和排出的出口路径中来形成水凝胶组合物。 当中断P（A）的施加时，液体冲洗剂被自动地输送到出口路径中，经受基本上恒定地施加的清除压力P（P），以从出口路径连续冲洗剩余的水凝胶组合物。

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