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141.发明申请METHODS OF IN VIVO ENGINEERING OF LARGE SEQUENCES USING MULTIPLE CRISPR/CAS SELECTIONS OF RECOMBINEERING EVENTS 审中-公开使用多重CRISPR / CAS选择重组事件的大型序列的生物工程方法公开(公告)号：US20160024529A1
公开(公告)日：2016-01-28
申请号：US14773466
申请日：2014-01-07
IPC分类号： C12N15/90
CPC分类号： C12N15/902 , C12N9/22 , C12N15/8509 , C12N2800/80
摘要： The present invention provides a method for making a large nucleic acid having a defined sequence in vivo. The method combines recombineering techniques with a CRISPR/Cas system to permit multiple insertions of defined sequences into a target nucleic acid at one time, double stranded cleavage of target nucleic acids in which the defined sequences were not successfully inserted, and selection of successful recombinant cells. The method further includes repeating the process one or more times, using a successful recombinant from one round as the host cell for the next round.
摘要翻译： 本发明提供一种在体内制备具有规定序列的大核酸的方法。 该方法将重组技术与CRISPR / Cas系统结合，以允许一次将定义的序列多次插入靶核酸，其中定义的序列未成功插入的靶核酸的双链切割，以及成功重组细胞的选择 。 该方法还包括使用来自一轮的成功重组作为下一轮的宿主细胞重复该过程一次或多次。
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公开(公告)号：US09247629B2
公开(公告)日：2016-01-26
申请号：US13838474
申请日：2013-03-15
发明人： Mehrnoosh Vahidpour , Geraint Owen
CPC分类号： H05H1/26 , H05H1/30 , H05H1/46 , H05H2001/4622
摘要： An apparatus includes an electromagnetic waveguide; an iris structure providing an iris in the waveguide. The iris structure may define an iris hole, a first iris slot at a first side of the iris hole, and a second iris slot at a second side of the iris hole. A plasma torch is disposed within the iris hole. An electric field in the waveguide changes direction from the first iris slot to the second iris slot. The plasma torch generates a plasma which is substantially symmetrical around a longitudinal axis of the plasma torch, such that the plasma may have a substantially toroidal shape. In some embodiments, a dielectric material is disposed in the iris hole, outside of the plasma torch. In some embodiments, the height of at least one of the iris slots is greater at the ends thereof than in the middle.
摘要翻译： 一种装置包括电磁波导; 在波导中提供虹膜的虹膜结构。 虹膜结构可以限定虹膜孔，在虹膜孔的第一侧的第一虹膜槽和在虹膜孔的第二侧的第二虹膜槽。 等离子体焰炬设置在虹膜孔内。 波导中的电场改变从第一光圈槽到第二光圈槽的方向。 等离子体焰炬产生基本上围绕等离子体焰炬的纵向轴对称的等离子体，使得等离子体可以具有基本上环形的形状。 在一些实施例中，电介质材料设置在等离子体焰炬的外部的虹膜孔中。 在一些实施例中，至少一个虹膜槽的高度在其端部比在中间大。
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公开(公告)号：US20160017405A1
公开(公告)日：2016-01-21
申请号：US14868961
申请日：2015-09-29
发明人： Michael Borns
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6832 , C12Q2527/125 , C12Q2527/137
摘要： The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
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公开(公告)号：US09236235B2
公开(公告)日：2016-01-12
申请号：US12277198
申请日：2008-11-24
申请人： Felician Muntean , Urs Steiner
发明人： Felician Muntean , Urs Steiner
IPC分类号： H01J49/06
CPC分类号： H01J49/063
摘要： An ion guide includes a plurality of curved electrodes and an ion deflecting device. The electrodes are arranged in parallel with each other and with a central curved axis, the curved central axis being co-extensive with an arc of a circular section having a radius of curvature, each electrode being radially spaced from the curved central axis, wherein the plurality of electrodes define a curved ion guide region arranged about the curved central axis and between opposing pairs of the electrodes. The ion deflecting device may include a device for applying a DC electric field to two or more of the electrodes in a radial direction. The ion deflecting device may include a pair of curved, parallel ion deflecting electrodes, which are in addition to curved electrodes utilized for applying an RF ion guiding field.
摘要翻译： 离子导向器包括多个弯曲电极和离子偏转装置。 电极彼此平行并具有中心的弯曲轴线，弯曲的中心轴与具有曲率半径的圆形截面的弧共同延伸，每个电极与弯曲的中心轴径向间隔开，其中， 多个电极限定围绕弯曲的中心轴线布置并且在相对的电极对之间布置的弯曲离子引导区域。 离子偏转装置可以包括用于沿径向向两个或更多个电极施加DC电场的装置。 离子偏转装置可以包括一对弯曲的平行离子偏转电极，这些电极除了用于施加RF离子引导场的弯曲电极之外。
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公开(公告)号：US09222876B2
公开(公告)日：2015-12-29
申请号：US13121148
申请日：2009-09-23
申请人： Thomas Lloyd Remsen
IPC分类号： G01N21/00 , G01N21/01 , G01N33/36 , G01J1/04 , G01N21/05 , G01N21/53 , G01N21/47 , G01N21/51
CPC分类号： G01N21/05 , G01N21/53 , G01N2021/4716 , G01N2021/513
摘要： A flow cell device includes a body having a hollow bore in which a liquid sample may reside. An electromagnetic beam may be directed through the body and into the bore to irradiate the liquid sample. The beam may be directed orthogonal to an axis of the bore. A light ray produced as a result of the irradiation may likewise be directed orthogonal to the bore axis along the same plane as the beam, and received by a detector. The body may be secured between a liquid inlet structure and a liquid outlet structure.
摘要翻译： 流动池装置包括具有中空孔的主体，液体样本可以位于该中空孔中。 电磁波束可以被引导穿过身体并进入孔中以照射液体样品。 梁可以垂直于孔的轴线定向。 作为照射结果产生的光线同样可以沿与波束相同的平面垂直于孔轴线，并由检测器接收。 主体可以固定在液体入口结构和液体出口结构之间。
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公开(公告)号：US09200885B2
公开(公告)日：2015-12-01
申请号：US14561425
申请日：2014-12-05
发明人： Gregg Ressler , Jeffrey H. Saller
CPC分类号： G01B9/02 , G01B9/02015 , G01J3/06 , G01J3/45 , G01J3/4535 , G01J2003/061 , G01N21/35 , G01N2021/3595 , G01N2201/061 , G01N2201/10 , G02B7/182
摘要： An interferometer includes a first assembly having a base, a beam splitter assembly to split light into first and second portions, and a fixed mirror for reflecting the first portion of light; and a second assembly movable with respect to the first assembly, and having first and second scan carriages, and a movable mirror connected to the second scan carriage for reflecting the second portion of light. The beam splitter assembly combines the reflected first and second portions of light into a recombined radiation beam. Inner bearing flexures enable movement of the first scan carriage relative to the base, and outer bearing flexures enable movement of the second scan carriage relative to the first scan carriage, such that a plane containing the movable mirror is maintained parallel to multiple planes containing the movable mirror at respective distances between the second and first assemblies during scan movement of the movable mirror.
摘要翻译： 干涉仪包括具有底座的第一组件，将光分成第一和第二部分的分束器组件以及用于反射第一部分光的固定镜; 以及第二组件，其可相对于所述第一组件移动，并且具有第一和第二扫描支架，以及连接到所述第二扫描托架的用于反射所述第二部分光的可移动反射镜。 分束器组件将反射的第一和第二部分光合并成复合辐射束。 内部轴承挠曲能够使第一扫描滑架相对于基座移动，而外轴承挠曲使第二扫描滑架相对于第一扫描滑架能够移动，使得包含可移动反射镜的平面保持平行于包含可移动的多个平面的多个平面 在可移动镜的扫描运动期间在第二组件和第一组件之间的相应距离处的镜子。
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公开(公告)号：US20150323532A1
公开(公告)日：2015-11-12
申请号：US14273320
申请日：2014-05-08
发明人： May Tom-Moy , Nazumi Alice Yamada
IPC分类号： G01N33/569
CPC分类号： G01N33/56966 , G01N33/582 , G01N2333/976
摘要： A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided.
摘要翻译： 提供了一种用于标记组织切片的方法。 在某些实施方案中，该方法可以包括：（a）使用包含第一一抗和第一标记的二抗的第一组标记试剂标记福尔马林固定的石蜡包埋的（FFPE）组织切片; （b）用蛋白酶处理标记的组织，从而消化第一个一抗和/或第一个标记的二次抗体，并从FFPE组织切片分离标记物; （c）洗涤组织切片以除去分离的标签和蛋白酶; 和（c）使用包含第二个一级抗体和第二个标记的第二抗体的第二组标记试剂来标记FFPE组织切片。 还提供了用于执行该方法的套件。
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公开(公告)号：US09151738B2
公开(公告)日：2015-10-06
申请号：US13898867
申请日：2013-05-21
IPC分类号： G01N33/18
CPC分类号： G01N33/1833 , Y10T436/21 , Y10T436/24 , Y10T436/255
摘要： Solvent extraction to quantify contamination of a sample by a hydrocarbon contaminant is performed by providing a defined quantity of the sample, providing a defined quantity of a siloxane solvent, mixing the defined quantity of the siloxane solvent and the defined quantity of the sample to extract the hydrocarbon contaminant from the sample to form a contaminant solution with the siloxane solvent, and separating the contaminant solution from the sample. A concentration of the hydrocarbon contaminant in the contaminant solution can be measured by vibrational spectroscopy. Siloxane solvents are CFC free, VOC exempt, odorless, colorless, low to moderately flammable, non-toxic, and safe for incidental skin contact. Some are even used in cosmetic products.
摘要翻译： 通过提供限定量的样品，提供限定量的硅氧烷溶剂，混合限定量的硅氧烷溶剂和限定量的样品来提取样品以污染样品以污染烃类污染物的溶剂萃取 烃污染物从样品中形成与硅氧烷溶剂的污染物溶液，并将污染物溶液与样品分离。 污染物溶液中的烃污染物的浓度可以通过振动光谱法测量。 硅氧烷溶剂不含氟氯化碳，挥发性有机化合物豁免，无臭，无色，低至中等易燃，无毒，安全可以偶发皮肤接触。 有些甚至用于化妆品。
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公开(公告)号：US09117617B2
公开(公告)日：2015-08-25
申请号：US13925623
申请日：2013-06-24
CPC分类号： H01J27/024 , H01J27/205 , H01J49/147
摘要： An ion source is configured for electron ionization and produces coaxial electron and ion beams. The ion source includes an ionization chamber along an axis, a magnet assembly configured for generating an axial magnetic field in the ionization chamber, an electron source, and a lens assembly configured for directing the ion beam out from the ionization chamber along the axis, reflecting the electron beam back toward the electron source, and transmitting higher energy ions out from the ion source while reflecting lower energy ions toward a lens element for neutralization.
摘要翻译： 离子源被配置用于电子电离并产生同轴电子和离子束。 离子源包括沿着轴线的电离室，被配置用于在电离室中产生轴向磁场的磁体组件，电子源和用于将离子束沿着轴线从电离室引出的透镜组件，反射 电子束返回到电子源，并从离子源发射较高能量的离子，同时将较低能量的离子反射向透镜元件进行中和。
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公开(公告)号：US09110053B2
公开(公告)日：2015-08-18
申请号：US12860669
申请日：2010-08-20
IPC分类号： G01N1/30 , G01N33/52 , B01L3/00 , G01N33/558 , G01N1/28
CPC分类号： G01N33/523 , B01L3/5023 , B01L2300/021 , B01L2300/126 , G01N33/558 , G01N2001/288
摘要： A dried blood-spotting device includes a carrier card having a window therethrough along with a punchable glass fiber paper disposed in the window for a uniform absorption of a blood droplet sample into a homogeneous circular sport. The glass fiber paper is configured for enabling improved analysis for small molecules by liquid chromatography/mass spectrometry of punched specimens of the sample absorbed glass fiber paper.
摘要翻译： 干血点装置包括具有穿过其中的窗口的载体卡片以及设置在窗口中的可冲洗玻璃纤维纸，以将血液滴样品均匀地吸收到均匀的圆形运动中。 玻璃纤维纸被配置用于通过样品吸收的玻璃纤维纸的冲压样品的液相色谱/质谱法来改进对小分子的分析。
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