公开(公告)号：US09193760B2

公开(公告)日：2015-11-24

申请号：US13237294

申请日：2011-09-20

Applicant: Peter S. Gagnon

Inventor： Peter S. Gagnon

IPC: C07K1/00 ,  C07K1/18 ,  B01D15/38 ,  C07K1/22 ,  B01D15/36

CPC classification number: C07K1/18 ,  B01D15/362 ,  B01D15/3828 ,  C07K1/22

Abstract: The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule.

Abstract translation: 本发明提供了使用磷灰石层析载体来分离和去除与生物制品复合的污染物的方法和材料。 本发明还提供用于解离靶生物分子的聚集体或不正确折叠的靶分子以提高靶分子纯化的材料和方法。

公开(公告)号：US09164058B2

公开(公告)日：2015-10-20

申请号：US14203351

申请日：2014-03-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lei Li ,  Xuemei Yang ,  Christopher Belisle

IPC: G01N27/447 ,  C08F2/46 ,  C07K1/26 ,  B65D85/00 ,  B01D57/02 ,  C08K3/00

CPC classification number: G01N27/44747 ,  B01D57/02 ,  B65D85/70 ,  C07K1/26 ,  C08F2/46 ,  C08K3/00 ,  G01N21/6428

Abstract: Hand cast gels, and solutions for their preparation, are described.

Abstract translation: 描述了手铸凝胶及其制备方法。

公开(公告)号：US20150286043A1

公开(公告)日：2015-10-08

申请号：US14674520

申请日：2015-03-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： NICHOLAS DOE

IPC: G02B21/08 ,  G02B21/36

CPC classification number: G02B21/084 ,  G02B21/14 ,  G02B21/361 ,  G02B21/367

Abstract: The present invention relates in general to microscopy systems and the illumination of target regions of the microscopy system with an annular illumination structure. The annular illumination structure can partially illuminate by quadrant, and can include a plurality of LEDs as light sources located in the annular structure that surround the lens or camera of an optical imaging and capture system.

Abstract translation: 本发明一般涉及显微镜系统和具有环形照明结构的显微镜系统的目标区域的照明。 环形照明结构可以通过象限部分地照亮，并且可以包括多个LED，其定位在围绕光学成像和捕获系统的透镜或照相机的环形结构中的光源。

公开(公告)号：US09134301B2

公开(公告)日：2015-09-15

申请号：US13298847

申请日：2011-11-17

Applicant: Robert M. Archer ,  Frank Hsiung ,  Paul Patt

Inventor： Robert M. Archer ,  Frank Hsiung ,  Paul Patt

IPC: G01N33/50 ,  G01N15/06 ,  G01N15/14 ,  G01N15/02

CPC classification number: G01N33/5094 ,  G01N15/0606 ,  G01N15/1463 ,  G01N2015/0288 ,  G01N2015/149

Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

Abstract translation: 具有仅在粘附状态下可检测的特征的粘附细胞可以根据这些特征独立于其粘附状态进行分类，通过将可变形标记应用于整个细胞群体，具有感兴趣特征的那些， 在其粘附状态下并且识别感兴趣的细胞在贴壁表面上的位置。 然后选择性地处理感兴趣的细胞或所有不同于感兴趣的细胞的细胞，以转化标记并实现感兴趣的细胞和剩余的细胞之间的分化。 然后将所有细胞从粘附状态释放并以与非贴壁细胞相同的方式进行分选，但是基于标记是否被转化或未转化。

公开(公告)号：US09001200B2

公开(公告)日：2015-04-07

申请号：US13004718

申请日：2011-01-11

Applicant: Gene Breniman ,  Michael Griffin

Inventor： Gene Breniman ,  Michael Griffin

IPC: H04N7/18 ,  G06K9/00 ,  G02B7/36 ,  G06T5/00 ,  G06T5/50 ,  G06T7/00

CPC classification number: G06K9/00134 ,  G02B7/36 ,  G06K9/00147 ,  G06T5/003 ,  G06T5/50 ,  G06T7/0012 ,  G06T2207/20076 ,  G06T2207/20221 ,  G06T2207/30024

Abstract: A system for characterizing cells takes a series of digital images of a sample containing the cells. Each of the images is taken at a different plane of focus. One of the images is determined to have been taken at a plane of best focus. The system analyzes the digital image taken at the plane of best focus and at least one other of the digital images to classify cells in the sample as either live or dead.

Abstract translation: 用于表征单元的系统采用包含单元的样本的一系列数字图像。 每个图像在不同的对焦平面拍摄。 其中一个图像被确定为在最佳焦点的平面上拍摄。 该系统分析在最佳对焦平面拍摄的数字图像和至少另一个数字图像，以将样本中的细胞分类为活或死。

公开(公告)号：US08980200B2

公开(公告)日：2015-03-17

申请号：US13923622

申请日：2013-06-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Daniel N. Fox ,  Matthias J. G. Ottenberg ,  Kevin P. Raley ,  Nathan Michael Gaskill-Fox

IPC: B01L99/00 ,  B01L3/00 ,  G01N15/14

CPC classification number: B01L3/56 ,  G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1413 ,  Y10T29/49826

Abstract: Disclosed is a nozzle assembly that is compact in size and that uses plastic tubing as an injection needle. Standard plastic fittings are utilized, which are inexpensive and widely available. The nozzle assembly has a simple construction and can be easily assembled and disassembled in a few minutes by a user. Cleaning and/or replacement of parts is inexpensive. Plastic tubing can be used as an injection needle that has superior qualities over commonly used stainless steel injection needles. Flexure of the injection needle tubing is prevented because of the compact size of the nozzle cavity.

Abstract translation: 公开了一种尺寸紧凑并且使用塑料管作为注射针的喷嘴组件。 使用标准的塑料配件，其便宜且广泛可用。 喷嘴组件具有简单的结构，并且可以在几分钟内由用户容易地组装和拆卸。 清洁和/或更换零件便宜。 塑料管可用作注射针，其具有比常用的不锈钢注射针优异的质量。 由于喷嘴腔的紧凑尺寸，防止注射针管弯曲。

公开(公告)号：US08956823B2

公开(公告)日：2015-02-17

申请号：US12184775

申请日：2008-08-01

Applicant: William F. Link ,  Renato B. Del Rosario ,  Randy Sweet ,  David L. King

Inventor： William F. Link ,  Renato B. Del Rosario ,  Randy Sweet ,  David L. King

IPC: G01N33/00 ,  G01N33/53 ,  G01N33/566 ,  C12N11/00 ,  G01N33/532 ,  G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/532

Abstract: An anti-antibody reagent for use in a competitive or sandwich simplex or multiplex assay, said reagent comprising one or more labeled anti-antibodies for the primary antibodies to be determined in the assay, the reagent further comprising a corresponding unlabeled anti-antibody in an excess or near excess concentration with respect to their binding partners.

Abstract translation: 一种用于竞争性或夹心单形或多重测定中的抗抗体试剂，所述试剂包含一种或多种在测定中待测定的一级抗体的标记抗体，该试剂还包含相应的未标记抗体 相对于其结合配偶体过量或接近过量的浓度。

公开(公告)号：US08945360B2

公开(公告)日：2015-02-03

申请号：US12691440

申请日：2010-01-21

Applicant: Shane Petersen ,  Cory Panattoni ,  Craig Rowell

Inventor： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

IPC: C07K1/26 ,  G01N27/447

CPC classification number: G01N27/44747 ,  C07K1/26 ,  Y10S436/826

Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

Abstract translation: 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。

公开(公告)号：US08907059B2

公开(公告)日：2014-12-09

申请号：US12615080

申请日：2009-11-09

Applicant: Ning Liu ,  Katrina Academia ,  Aran Paulus ,  Tim Wehr ,  John A. Walker, II ,  Steve Freeby

Inventor： Ning Liu ,  Katrina Academia ,  Aran Paulus ,  Tim Wehr ,  John A. Walker, II ,  Steve Freeby

IPC: C07K1/14 ,  B01J20/04 ,  B01D15/38 ,  B01J20/282 ,  C07K1/16

CPC classification number: B01J20/048 ,  B01D15/3847 ,  B01J20/282 ,  C07K1/14 ,  C07K1/165

Abstract: Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput.

Abstract translation: 通过在陶瓷羟基磷灰石上分级，从生物液体和其他肽混合物的消化物中提取磷酸化肽。 陶瓷羟基磷灰石易于在离心机中使用，允许大量小体积样品的快速分级，并因此高通量。

公开(公告)号：US08895707B2

公开(公告)日：2014-11-25

申请号：US13205354

申请日：2011-08-08

Applicant: Larry J. Cummings

Inventor： Larry J. Cummings

IPC: A23J1/00 ,  B01D15/38 ,  B01D15/36 ,  C07K1/22 ,  C07K1/20 ,  C07K1/18 ,  B01D15/42

CPC classification number: C07K1/18 ,  B01D15/361 ,  B01D15/3804 ,  B01D15/426 ,  C07K1/20 ,  C07K1/22

Abstract: Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.

Abstract translation: 通过含有受控量的钙和磷酸根离子的洗脱缓冲液，从树脂中洗脱已经保留在羟基磷灰石树脂上用于蛋白质分离，纯化或两者的蛋白质，包括单克隆抗体。 缓冲液允许在酸性pH下反复进行洗脱，而不会使树脂劣化。