公开(公告)号：US08053222B2

公开(公告)日：2011-11-08

申请号：US12370118

申请日：2009-02-12

Applicant: Po-Huang Liang ,  Chih-Jung Kuo ,  Yan-Ping Shih

Inventor： Po-Huang Liang ,  Chih-Jung Kuo ,  Yan-Ping Shih

IPC: C12N9/48 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04

CPC classification number: C12P21/06 ,  C12N9/506

Abstract: A mutated severe acute respiratory syndrome-associated coronavirus 3C-like protease and use thereof for cleaving a protein that includes a cleavage site recognizable by the mutated protease to yield a polypeptide fragment of interest.

Abstract translation: 突变的严重急性呼吸综合征相关冠状病毒3C样蛋白酶及其用于切割包括可被突变蛋白酶识别的切割位点以产生感兴趣的多肽片段的蛋白质的用途。

公开(公告)号：US08034910B2

公开(公告)日：2011-10-11

申请号：US12436211

申请日：2009-05-06

Applicant: Ting-Fang Wang

Inventor： Ting-Fang Wang

IPC: C07H21/02 ,  C12P21/06 ,  A23J1/00

CPC classification number: C07K1/22 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/50 ,  C12P21/02 ,  C12P21/06

Abstract: A simple and efficient SUMO fusion protein expression system for producing native proteins.

Abstract translation: 一种用于产生天然蛋白质的简单有效的SUMO融合蛋白表达系统。

公开(公告)号：US08024277B2

公开(公告)日：2011-09-20

申请号：US12131162

申请日：2008-06-02

Applicant: Sai-Ping Li ,  Sun-Chong Wang

Inventor： Sai-Ping Li ,  Sun-Chong Wang

IPC: G06F17/00

CPC classification number: G06F19/12 ,  G06F19/20 ,  G06F19/24

Abstract: Gene regulation network is reconstructed using time series microarray data under the method of the Bayesian network. Particular power-law function is used to calculate the joint probabilities among genes across time points. This invention discloses the use of the downhill simplex algorithm to find global maxima of interrelational likelihood. Arcs with higher frequencies are selected to establish the gene regulation network. Prior knowledge may be included into candidate gene networks to accelerate search for best networks.

Abstract translation: 基于贝叶斯网络的方法，使用时序微阵列数据重建基因调控网络。 特定的幂律函数用于计算时间点之间基因的联合概率。 本发明公开了使用下坡单纯形算法来找到相关似然性的全局最大值。 选择具有较高频率的弧以建立基因调控网络。 可能将先验知识纳入候选基因网络，以加速搜索最佳网络。

公开(公告)号：US07998482B2

公开(公告)日：2011-08-16

申请号：US12533086

申请日：2009-07-31

Applicant: Chi-Huey Wong ,  Shie-Liang Hsieh ,  Tsui-Ling Hsu ,  Shih-Chin Cheng ,  Szu-Ting Chen

Inventor： Chi-Huey Wong ,  Shie-Liang Hsieh ,  Tsui-Ling Hsu ,  Shih-Chin Cheng ,  Szu-Ting Chen

IPC: A61K39/395 ,  C12N7/00 ,  A61K39/285

CPC classification number: C07K16/2851 ,  C07K2317/76 ,  C07K2319/30 ,  G01N33/56972 ,  G01N33/56983 ,  G01N2333/18 ,  G01N2500/02 ,  Y02A50/53

Abstract: The disclosure provides fusion proteins comprising a carbohydrate recognition domain of an innate immunity receptor and a heterologous polypeptide. The fusion proteins of the disclosure may be used, for example, to fingerprint polysaccharide compositions and to purify polysaccharide compositions. Polysaccharide compositions include those isolated from Ganoderma lucidum (Reishi). The methods and reagents of the disclosure may also be used to identify innate immunity receptors and cell types that bind to polysaccharide compositions (including polysaccharide compositions associated with pathogens), whereupon modulators of the identified receptors can then be obtained. The fusion proteins also may be used to inhibit the interaction between a polysaccharide composition and an innate immunity receptor on a cell surface. The methods and reagents of the disclosure are used in one example to determine that the DLVR1 innate immunity receptor on macrophages interacts with Dengue virus (DV), and that DLVR1 is responsible for DV-mediated secretion of proinflammatory cytokines from macrophages. The disclosure also provides DVLR1 antibodies that prevent the secretion of proinflammatory cytokines by DV-infected macrophages.

Abstract translation: 本公开提供了包含先天免疫受体的碳水化合物识别结构域和异源多肽的融合蛋白。 本公开的融合蛋白可以用于例如指纹多糖组合物和纯化多糖组合物。 多糖组合物包括从灵芝（Reishi）分离的那些。 本公开的方法和试剂也可用于鉴定结合多糖组合物（包括与病原体相关的多糖组合物）的先天免疫受体和细胞类型，于是可以获得鉴定的受体的调节剂。 融合蛋白也可用于抑制多糖组合物和细胞表面上的先天免疫受体之间的相互作用。 在一个实例中使用本公开的方法和试剂来确定巨噬细胞上的DLVR1先天免疫受体与登革热病毒（DV）相互作用，并且DLVR1负责来自巨噬细胞的DV介导的促炎细胞因子的分泌。 本公开还提供DVLR1抗体，其防止DV感染的巨噬细胞分泌促炎细胞因子。

公开(公告)号：US07991454B2

公开(公告)日：2011-08-02

申请号：US10435717

申请日：2003-05-08

Applicant: Yeu-Kuang Hwu ,  Giorgio Margaritondo ,  Jung Ho Je

Inventor： Yeu-Kuang Hwu ,  Giorgio Margaritondo ,  Jung Ho Je

IPC: A61B6/00

CPC classification number: A61B6/482 ,  A61B6/4092 ,  A61B6/481 ,  A61B6/484 ,  A61B6/504 ,  A61B6/508

Abstract: Images of blood vessels of a body are obtained by injecting a refraction enhancement contrast agent into the blood vessels to increase a difference in refractive indices of the blood vessels and surrounding material. The blood vessels are irradiated with a penetrating radiation, and an image of the blood vessels is generated based on detected radiation. The image has visible edge enhancement features indicating boundaries of the blood vessels.

Abstract translation: 通过将折射增强造影剂注射到血管中以增加血管和周围材料的折射率差异来获得身体血管的图像。 用穿透性辐射照射血管，并且基于检测到的辐射产生血管的图像。 该图像具有指示血管边界的可见边缘增强特征。

公开(公告)号：US20110177033A1

公开(公告)日：2011-07-21

申请号：US13008279

申请日：2011-01-18

Applicant: Che-Kun James SHEN ,  Ruei-Lin Chen ,  Yu-Chi Chou

Inventor： Che-Kun James SHEN ,  Ruei-Lin Chen ,  Yu-Chi Chou

IPC: A61K35/76 ,  C12Q1/68 ,  C12N5/078 ,  A61K31/7088 ,  A61P7/06 ,  A61P7/00

CPC classification number: C12Q1/6883 ,  C12Q2600/136

Abstract: A method of screening compounds capable of activating ζ and/or ε globin gene promoter activity in an erythroid cell is disclosed. The methods comprises contacting in a medium a compound to be screened with RREB1; wherein the medium comprises a polynucleotide comprising the nucleotide sequence of 5′-M-C-M-C-A-M-M-H-M-M-M-3′, wherein M is the nucleotide adenine or cytosine, and H is the nucleotide adenine, cytosine or thymine; the RREB1 bindable to the polynucleotide; determining binding of the compound to the RREB1; and determining change in binding of the RREB1 to the polynucleotide; wherein detection of binding of the compound to the RREB1 and change in binding of the RREB1 to the polynucleotide is indicative that the compound is capable of activating ζ and/or ε globin gene promoter activity. Also disclosed is a method of activating ζ and/or ε globin gene promoter activity in an erythroid cell.

Abstract translation: 一种筛选能够激活ζ和/或/ 公开了红细胞中的珠蛋白基因启动子活性。 所述方法包括在培养基中接触待筛选的化合物与RREB1; 其中所述培养基包含含有5'-M-C-M-C-A-M-M-H-M-M-M-3'核苷酸序列的多核苷酸，其中M为核苷酸腺嘌呤或胞嘧啶，H为核苷酸腺嘌呤，胞嘧啶或胸腺嘧啶; 可与该多核苷酸结合的RREB1; 确定化合物与RREB1的结合; 并确定RREB1与多核苷酸结合的变化; 其中所述化合物与RREB1的结合的检测和RREB1与多核苷酸的结合的变化表明该化合物能够激活ζ和/或/ 珠蛋白基因启动子活性。 还公开了一种激活ζ和/或egr的方法; 珠蛋白基因启动子在红细胞中的活性。

公开(公告)号：US07981639B2

公开(公告)日：2011-07-19

申请号：US10782287

申请日：2004-02-19

Applicant: Jei-Fu Shaw ,  Guan-Chiun Lee ,  Jen-Jye Chen

Inventor： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Jen-Jye Chen

IPC: A23L1/105 ,  C12G1/022

CPC classification number: C12P19/14 ,  C12P19/20 ,  C12P19/22 ,  C12P19/24

Abstract: A method for producing a monosaccharide-rich syrup from starch-containing produce. The method includes treating a starch-containing produce slurry with a first starch hydrolyzing enzyme that hydrolyzes starch to oligosaccharide and a second starch hydrolyzing enzyme that hydrolyzes starch or oligosaccharide to glucose. The starch-containing produce can be further treated with an enzyme that converts glucose to other monosaccharides, or treated with a microorganism that converts glucose to a fermentation product. Also within the scope of this invention is a method for producing a syrup rich in a disaccharide, such as trehalose.

Abstract translation: 从含淀粉的产品制备富含糖的糖浆的方法。 该方法包括用水解淀粉至寡糖的第一淀粉水解酶和将淀粉或寡糖水解成葡萄糖的第二淀粉水解酶处理含淀粉的产物浆液。 含淀粉的产物可以用将葡萄糖转化为其他单糖的酶进一步处理，或用将葡萄糖转化为发酵产物的微生物处理。 也在本发明的范围内的是生产富含二糖的糖浆如海藻糖的方法。

公开(公告)号：US07977666B2

公开(公告)日：2011-07-12

申请号：US12453088

申请日：2009-04-29

Applicant: Shiang-Yu Wang ,  Hong-Shi Ling ,  Ming-Cheng Lo ,  Chien-Ping Lee

Inventor： Shiang-Yu Wang ,  Hong-Shi Ling ,  Ming-Cheng Lo ,  Chien-Ping Lee

IPC: H01L29/06 ,  H01L31/0328 ,  H01L31/0336 ,  H01L31/072 ,  H01L31/109

CPC classification number: H01L31/035236 ,  B82Y20/00 ,  H01L31/03046 ,  H01L31/105 ,  Y02E10/544 ,  Y02P70/521 ,  Y10S977/759

Abstract: The present invention is disclosed that a device capable of normal incident detection of infrared light to efficiently convert infrared light into electric signals. The device includes a substrate, a first contact layer formed on the substrate, an active layer formed on the first contact layer, a barrier layer formed on the active layer and a second contact layer formed on the barrier layer, wherein the active layer includes multiple quantum dot layers.

Abstract translation: 公开了能够正常入射检测红外光以有效地将红外光转换为电信号的装置的本发明。 该器件包括衬底，形成在衬底上的第一接触层，形成在第一接触层上的有源层，形成在有源层上的势垒层和形成在阻挡层上的第二接触层，其中有源层包括多个 量子点层。

公开(公告)号：US20110165279A1

公开(公告)日：2011-07-07

申请号：US12985960

申请日：2011-01-06

Applicant: Yaw-Huei LIN ,  Guan-Jhong HUANG

Inventor： Yaw-Huei LIN ,  Guan-Jhong HUANG

IPC: A61K36/00 ,  A61P29/00

CPC classification number: A61K36/39

Abstract: Some embodiments of the invention are directed to a pharmaceutical composition comprising an anti-inflammatory or an anti-hyperalgesic effective amount of isolated or purified Ipomomoea batatas trypsin inhibitor and a pharmaceutically acceptable carrier. The Ipomomoea batatas trypsin inhibitor may be derived from a sweet potato leaf or storage root. Also contemplated are methods of treating inflammation or hyperalgesia comprising selecting a subject suffering from inflammation or hyperalgesia, such as a subject with edema; and administering an anti-inflammatory or anti-hyperalgesia effective amount of amount Ipomomoea batatas trypsin inhibitor or a composition comprising an amount of Ipomomoea batatas trypsin inhibitor to the subject; wherein inflammation or hyperalgesia in the subject is reduced upon administration.

Abstract translation: 本发明的一些实施方案涉及药物组合物，其包含抗炎或抗痛觉过敏有效量的分离或纯化的伊曲霉胰蛋白酶抑制剂和药学上可接受的载体。 Ipomomoea batatas胰蛋白酶抑制剂可以衍生自甘薯叶或储藏根。 还考虑了治疗炎症或痛觉过敏的方法，其包括选择患有炎症或痛觉过敏的受试者，例如具有水肿的受试者; 以及将有效量的抗炎或抗痛觉过敏剂量的益母草胰蛋白酶抑制剂或包含一定量的伊曲霉巴斯德胰蛋白酶抑制剂的组合物施用于受试者; 其中所述受试者的炎症或痛觉过敏在给药时减少。

公开(公告)号：US07972878B2

公开(公告)日：2011-07-05

申请号：US12478084

申请日：2009-06-04

Applicant: Yia-Chung Chang ,  David M T Kuo

Inventor： Yia-Chung Chang ,  David M T Kuo

IPC: H01L21/00 ,  H01L29/76

CPC classification number: H01L21/28273 ,  B82Y10/00 ,  B82Y30/00 ,  H01L29/122 ,  H01L29/127 ,  H01L29/42324 ,  H01L29/66977 ,  H01L29/7888 ,  Y10S438/962

Abstract: A method of making a quantum dot memory cell, the quantum dot memory cell including an array of quantum dots disposed between a first electrode and a second electrode, includes obtaining values for a tunneling current through the quantum dot memory cell as a function of a voltage applied to the quantum dot memory cell and selecting parameters of the quantum dot memory cell such that the tunneling current through the quantum dot memory cell exhibits a bistable current for at least some values of the voltage applied to the quantum dot memory cell. The values for the tunneling current are determined on the basis of a density of states of the array of quantum dots.

Abstract translation: 一种制造量子点存储单元的方法，包括设置在第一电极和第二电极之间的量子点阵列的量子点存储单元包括获得通过量子点存储单元的隧穿电流的值作为电压的函数 施加到量子点存储单元并且选择量子点存储单元的参数，使得通过量子点存储单元的隧穿电流对施加到量子点存储单元的电压的至少一些值呈现双稳态电流。 基于量子点阵列的状态密度来确定隧穿电流的值。