公开(公告)号：US20120077285A1

公开(公告)日：2012-03-29

申请号：US13257188

申请日：2010-03-25

Applicant: Janina Cramer

Inventor： Janina Cramer

IPC: G01N33/53 ,  C07H1/08 ,  H01F1/00 ,  C07K1/14 ,  C07H3/00 ,  B01D11/02 ,  C07H21/04 ,  C07K14/00

CPC classification number: G01N33/54326 ,  B03C1/00 ,  B03C2201/18 ,  C12N15/1013

Abstract: The present invention relates to a method for purifying biomolecules or for analyzing whether an aqueous phase contains biomolecules by means of magnetic separation. The invention further relates to uses, to devices, and to kits that relate to the method according to the invention.

Abstract translation: 本发明涉及一种纯化生物分子的方法或用于分析水相是否含有通过磁选分离的生物分子。 本发明还涉及与根据本发明的方法相关的用途，装置和套件。

公开(公告)号：US08132674B1

公开(公告)日：2012-03-13

申请号：US12760991

申请日：2010-04-15

Applicant: Dan Allore ,  John Godzik

Inventor： Dan Allore ,  John Godzik

IPC: B03C1/00

CPC classification number: B03C1/284 ,  B03C1/0332 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/28

Abstract: A tramp metal separation device includes a first housing having an inlet and an outlet, a first drawer and a second drawer. The first drawer and the second drawer each have a plurality of magnets and a wiper assembly, where each drawer is supported with respect to the first housing such that each first drawer is moveable between an extended position and a retracted position. In the extended position, the magnets of the respective drawer are positioned within the first housing and are adapted to be in contact with the stream of raw materials. In the retracted position, the magnets of the respective drawer are positioned outside of the first housing. The wiper assembly of each drawer removes contaminants from the magnets as the respective drawer moves from the extended position to the retracted position. Each of the first drawer and the second drawer is restrained against movement from the extended position to the retracted position when the other of the first drawer and the second drawer is disposed in the retracted position.

Abstract translation: 流动金属分离装置包括具有入口和出口的第一壳体，第一抽屉和第二抽屉。 第一抽屉和第二抽屉各自具有多个磁体和擦拭器组件，其中每个抽屉相对于第一壳体被支撑，使得每个第一抽屉可在延伸位置和缩回位置之间移动。 在延伸位置，相应抽屉的磁体定位在第一壳体内并适于与原料流接触。 在缩回位置，相应抽屉的磁体位于第一壳体的外部。 每个抽屉的刮水器组件随着相应的抽屉从延伸位置移动到缩回位置，从磁体中去除污染物。 当第一抽屉和第二抽屉中的另一个设置在缩回位置时，第一抽屉和第二抽屉中的每一个被限制为从延伸位置移动到缩回位置。

公开(公告)号：US20120045828A1

公开(公告)日：2012-02-23

申请号：US13284482

申请日：2011-10-28

Applicant: Ronald W. Davis ,  Stefanie S. Jeffrey ,  Michael N. Mindrinos ,  R. Fabian Pease ,  Ashley Ann Powell ,  AmirAli Hajhossein Talasaz

Inventor： Ronald W. Davis ,  Stefanie S. Jeffrey ,  Michael N. Mindrinos ,  R. Fabian Pease ,  Ashley Ann Powell ,  AmirAli Hajhossein Talasaz

IPC: C12N5/09 ,  C12M3/00

CPC classification number: G01N33/54333 ,  B03C1/01 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26

Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.

Abstract translation: 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置俘获的细胞在捕获后培养时显示出不降低的活力。 以适合于遗传分析的状态捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。

公开(公告)号：US08102636B2

公开(公告)日：2012-01-24

申请号：US12377609

申请日：2007-08-10

Applicant: Mark Thomas Johnson ,  Jacob Marinus Jan Den Toonder ,  Murray Fulton Gillies ,  Marc Wilhelmus Gijsbert Ponjee

Inventor： Mark Thomas Johnson ,  Jacob Marinus Jan Den Toonder ,  Murray Fulton Gillies ,  Marc Wilhelmus Gijsbert Ponjee

IPC: H01H47/00

CPC classification number: H01F7/064 ,  B01L3/5027 ,  B03C1/034 ,  B03C2201/18 ,  G09G3/3486 ,  G09G2300/0819 ,  G09G2300/0842 ,  G09G2300/0852 ,  G09G2320/043 ,  H01F2007/068

Abstract: The present invention relates to a magnetic field generation device comprising a magnetic field generating element (101) and a limiter (103) for limiting a magnitude of a current through the magnetic field generating element (101).

Abstract translation: 磁场产生装置技术领域本发明涉及一种包括用于限制通过磁场产生元件（101）的电流大小的磁场产生元件（101）和限制器（103）的磁场产生装置。

公开(公告)号：US20120012512A1

公开(公告)日：2012-01-19

申请号：US12998116

申请日：2009-07-20

Applicant: Vladimir Danov ,  Bernd Gromoll

Inventor： Vladimir Danov ,  Bernd Gromoll

IPC: B03C1/02

CPC classification number: B03C1/10 ,  B03C2201/18

Abstract: Ferromagnetic particles are separated from a suspension using a tubular reactor having at least one magnet, where a suspension is able to flow through the reactor. A displacer is arranged inside the reactor.

Abstract translation: 使用具有至少一个磁体的管式反应器将铁磁颗粒与悬浮液分离，其中悬浮液能够流过反应器。 置换器布置在反应器内部。

公开(公告)号：US08071054B2

公开(公告)日：2011-12-06

申请号：US12871788

申请日：2010-08-30

Applicant: Sang-Hyun Oh ,  Amarendra Kumar Singh ,  Yanting Zhang ,  Dharmakirthi Nawarathna ,  Unyoung Kim ,  Patrick Sean Daugherty ,  Hyongsok Soh ,  Brian Scott Ferguson

Inventor： Sang-Hyun Oh ,  Amarendra Kumar Singh ,  Yanting Zhang ,  Dharmakirthi Nawarathna ,  Unyoung Kim ,  Patrick Sean Daugherty ,  Hyongsok Soh ,  Brian Scott Ferguson

IPC: C12Q1/70

CPC classification number: B01L3/502776 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/043 ,  B03C1/32 ,  B03C2201/18

Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.

Abstract translation: 微流体装置可以采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 分离由磁电泳驱动。 分选站通常包括单独的缓冲器和采样流。 施加到分选站的磁场梯度将磁性粒子（其选择性地标记目标物种）的流动路径偏转成缓冲液流。 离开分选站的缓冲液流用于检测或进一步处理用磁性颗粒标记的纯化目标物种。

公开(公告)号：US20110287555A1

公开(公告)日：2011-11-24

申请号：US12783619

申请日：2010-05-20

Applicant: Douglas M. Campbell ,  Gregory E. Gardner

Inventor： Douglas M. Campbell ,  Gregory E. Gardner

IPC: G01N1/00 ,  B01J19/12

CPC classification number: G01N35/0098 ,  B01J2219/00452 ,  B01J2219/005 ,  B01J2219/00648 ,  B01J2219/00655 ,  B03C1/01 ,  B03C1/03 ,  B03C2201/18 ,  G01N2035/00574 ,  G01N2035/1088 ,  Y10T436/25

Abstract: An assembly that utilizes reusable sheaths for covering magnetic rods for collecting particles in an inverse magnetic particle process. The magnetic rod is removed from the reusable sheath to release particles at the same or a different location. The reusable sheaths can be assembled in a cylindrical plate, which can be rotated to position a clean sheath for each step of the inverse magnetic particle process. When not being used for particle separation, the sheaths can be washed of potentially contaminating solution in wash receptacles.

Abstract translation: 一种组件，其利用可重复使用的护套来覆盖用于在反向磁性粒子过程中收集粒子的磁棒。 将磁棒从可重复使用的护套中取出，以在相同或不同的位置释放颗粒。 可重复使用的护套可以组装在圆柱形板中，其可以旋转以对反向磁性颗粒过程的每个步骤定位干净的护套。 当不用于颗粒分离时，护套可以在洗涤容器中清洗潜在的污染溶液。

公开(公告)号：US20110263044A1

公开(公告)日：2011-10-27

申请号：US13056355

申请日：2008-07-22

Applicant: Ulrich Reidt ,  Alois Friedberger ,  Christoph Heller ,  Thomas Ziemann ,  Harald Waltenberger ,  Günter Müller

Inventor： Ulrich Reidt ,  Alois Friedberger ,  Christoph Heller ,  Thomas Ziemann ,  Harald Waltenberger ,  Günter Müller

IPC: G01N33/53

CPC classification number: B03C1/286 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C12M47/02 ,  G01N1/2202 ,  G01N1/2273 ,  G01N1/4077 ,  G01N35/0098

Abstract: An apparatus and a method for automatic detection of particles, in particular, biological particles such as micro-organisms. The apparatus has a device for binding the particles to separating particles which can be bound selectively to the particles. The apparatus further includes a separating device for extraction of the separating particles with bound particles from a collecting fluid, and a detection unit for detecting a number and/or concentration of the particles separated in this manner. The device for binding the particles to the separating particles includes a collecting device for collecting a collecting fluid including the separating particles and the particles that are provided by a particle-fluid mixture.

Abstract translation: 用于自动检测颗粒的设备和方法，特别是生物颗粒如微生物。 该装置具有用于将颗粒结合到可以选择性地结合到颗粒的分离颗粒的装置。 该装置还包括用于从收集流体中提取具有结合颗粒的分离颗粒的分离装置和用于检测以这种方式分离的颗粒的数量和/或浓度的检测单元。 用于将颗粒结合到分离颗粒的装置包括用于收集包括分离颗粒和由颗粒 - 流体混合物提供的颗粒的收集流体的收集装置。

公开(公告)号：US08021614B2

公开(公告)日：2011-09-20

申请号：US11449149

申请日：2006-06-08

Applicant: Lotien Richard Huang ,  Thomas A. Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

Inventor： Lotien Richard Huang ,  Thomas A. Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

IPC: G01N33/00

CPC classification number: B03C1/30 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0472 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/32 ,  B03C2201/18 ,  B33Y80/00 ,  C12M47/04 ,  C12M47/06 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/5044 ,  G01N2035/00237 ,  Y10T137/0318 ,  Y10T137/8593 ,  Y10T436/25 ,  Y10T436/25375

Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Abstract translation: 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。

公开(公告)号：US20110203932A1

公开(公告)日：2011-08-25

申请号：US12799597

申请日：2010-04-28

Applicant: Lev Nikolaevich Popov

Inventor： Lev Nikolaevich Popov

IPC: B03C5/02

CPC classification number: B03C1/288 ,  B03C2201/18 ,  B03C2201/30

Abstract: A device for magnetic treatment of a fluid flow preferably comprises a spirally-shaped conduit having spiral turns with a null step therebetween, and a cross-section for passing the flow therethrough, inner magnets internally circumferentially surrounding the turns coupled to the conduit, outer magnets externally circumferentially surrounding the turn. Each inner magnet is situated opposite to a respective counterpart outer magnet, so that the North (or South) pole of the inner magnet faces the South (or North) pole of the counterpart magnet. The magnets can be made of specific sizes, materials, covered by magnetic yokes. In a multi-layer embodiment, the device comprises a steel tube enclosed into and supporting an inner cylindrical magnet, a spirally-shaped conduit consisting of a number of layers, and rows of outer magnets consisting of magnets circumferentially surrounding predeterminedly chosen layers, and having magnetic fluxes uniformly directed either from or to the center of the cylindrical magnet.

Abstract translation: 流体流动的磁处理装置优选地包括具有螺旋形匝的螺旋形导管，其间具有零级步骤，以及用于使流过其的横截面，内部磁体内部周向围绕联接到导管的匝，外部磁体 从外部周向围绕转弯。 每个内磁体与相应的对应外磁体相对，使得内磁体的北（或南）极面对配对磁体的南（或北）极。 磁铁可以由特定的尺寸制成，材料由磁轭覆盖。 在多层实施例中，该装置包括封闭并支撑内部圆柱形磁体的钢管，由多个层组成的螺旋形导管和由周向围绕预定选择的层的磁体组成的一排外部磁体，并且具有 磁通量从圆柱形磁体的中心均匀地引导到圆柱形磁体的中心。