公开(公告)号：US20130287746A1

公开(公告)日：2013-10-31

申请号：US13979807

申请日：2011-02-17

Applicant: Ji Chul Yu ,  Se Ken Yeo ,  Tai Hyoung Kim ,  Dong Sam Shu ,  Cheong Ho Chang

Inventor： Ji Chul Yu ,  Se Ken Yeo ,  Tai Hyoung Kim ,  Dong Sam Shu ,  Cheong Ho Chang

IPC: C07K1/113 ,  A61L27/36 ,  A61L27/24 ,  C07K14/78

CPC classification number: C07K1/113 ,  A61K8/0212 ,  A61K8/65 ,  A61K47/42 ,  A61K2800/81 ,  A61L15/325 ,  A61L15/42 ,  A61L27/24 ,  A61L27/3604 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61L27/60 ,  A61Q19/00 ,  C07K14/78

Abstract: The present invention relates to radiation cross-linked collagen gel, and a preparation method and usage method thereof. To this end, the present invention comprises a cross-linked collagen material made by irradiating liquid collagen with radioactive rays, wherein the concentration of said collagen is specifically 0.1-10% (W/V), and the radiation dose (dose rate×time) is 0.1-40 kGy on the basis of 1 kGy/hr. The present invention configured as above can prepare a formulated collagen gel using a physical cross-linking method instead of a chemical cross-linking method, specifically carries out the formulation by mixing biocompatible materials, and provides a method capable of using a cross-linked collagen hydrogel in wound dressings, graft materials, cell cultures and the like. Therefore, the present invention provides an industrially convenient and safe preparation method, thereby instilling a good image to a customer by greatly improving the quality and confidence in the products.

Abstract translation: 本发明涉及辐射交联胶原凝胶及其制备方法及其使用方法。 为此，本发明包括通过用放射线照射液体胶原物制成的交联胶原材料，其中所述胶原蛋白的浓度特别是0.1-10％（W / V），辐射剂量（剂量率×时间 ）基于1kGy / hr为0.1-40kGy。 如上构造的本发明可以使用物理交联法代替化学交联法制备配制的胶原凝胶，特别是通过混合生物相容性材料进行制剂，并提供能够使用交联胶原蛋白 伤口敷料中的水凝胶，移植材料，细胞培养物等。 因此，本发明提供了工业上方便和安全的制备方法，从而通过大大提高产品的质量和信心向顾客灌注良好的形象。

公开(公告)号：US20100034783A1

公开(公告)日：2010-02-11

申请号：US12311783

申请日：2006-11-03

Applicant: Hyun-Mi Son ,  Cheong-Ho Chang ,  Jae-Deog Jang

Inventor： Hyun-Mi Son ,  Cheong-Ho Chang ,  Jae-Deog Jang

IPC: A61K35/12 ,  A61B10/00 ,  B65D81/18 ,  B65B7/28 ,  A61B5/15 ,  C12N5/02 ,  A01N1/02

CPC classification number: A61L27/3847 ,  A61F2/28 ,  A61F2/30756 ,  A61L27/3817 ,  A61L27/3821 ,  A61L27/3834 ,  A61L27/3852

Abstract: An aseptic/sterile medical kits are comprising a cartilage regeneration kit, a bone regeneration kit or an umbilical cord blood storage kit in a configuration that each process performs according to functionally-specialized kit sets for each step, via division of overall processes into corresponding steps for isolation, culture, collection and storage of cells, and implantation of desired cells into target sites of the body. The cartilage is regenerated by cartilage tissue collection; chondrocyte isolation; chondrocyte medium change and subculture; preparation of chondrocyte therapy product; media for isolation/culture/preparation/cryopreservation of cells; and media for isolation/culture/cryopreservation of cells, using the cartilage regeneration kit. The bone is regenerated by bone marrow collection; osteoblast isolation; osteoblast medium change and subculture; and preparation of osteoblast therapy product, using the bone regeneration kit. Additionally, the umbilical cord blood is stored by umbilical cord blood collection; hematopoietic stem cell isolation; and cryopreservation of hematopoietic stem cells, using the umbilical cord blood storage kit.

Abstract translation: 无菌/无菌医疗试剂盒包括软骨再生试剂盒，骨再生试剂盒或脐带血液存储试剂盒，其配置为每个过程根据功能上专门用于每一步骤的试剂盒进行，通过将整个过程分为对应步骤 用于细胞的分离，培养，收集和储存，以及将期望的细胞植入人体的靶位点。 软骨通过软骨组织收集再生; 软骨细胞分离; 软骨细胞培养基变化和亚文化; 软骨细胞治疗产品的制备; 介质用于分离/培养/制备/冷冻保存细胞; 和培养基用于细胞的分离/培养/冷冻保存，使用软骨再生试剂盒。 骨骼通过骨髓采集再生; 成骨细胞分离; 成骨细胞介质变化和亚文化; 并使用骨再生试剂盒制备成骨细胞治疗产品。 另外，脐带血通过脐带血采集储存; 造血干细胞分离; 并使用脐带血液储存试剂盒对造血干细胞进行冷冻保存。

公开(公告)号：US20090325275A1

公开(公告)日：2009-12-31

申请号：US12309868

申请日：2007-02-15

Applicant: Jeong-Yong Choi ,  Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Hyang-Soon Chang ,  Jin-Wook Chung ,  Cheong-Ho Chang

Inventor： Jeong-Yong Choi ,  Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Hyang-Soon Chang ,  Jin-Wook Chung ,  Cheong-Ho Chang

IPC: C12M1/18

CPC classification number: C12M37/00 ,  B01L1/04 ,  F24F3/1607

Abstract: Cell manipulation and cultivation equipment for the production of cell therapy products has developed for preventing contamination of cells and improving the utility of a space. The equipment includes a room for the production and testing of cell therapy products, the room having an inner space enclosed in all sides and an outer entrance/exit door provided at any one side thereof, an L-shaped partition installed in a position of the room for the production and testing of cell therapy products, the partition having an inner entrance/exit door provided at any one side thereof, and a clean bench device placed inside the partition, the clean bench device including first, second, and third clean benches for preventing contamination of cells and increasing the utility of a space to the maximum extent. The operating of the clean room and clean bench has advantages to prevent contamination and improving the quality and reliability of cell therapy products.

Abstract translation: 用于生产细胞治疗产品的细胞操作和培养设备已经开发用于防止细胞污染和改善空间的效用。 该设备包括用于生产和测试细胞治疗产品的房间，房间具有封闭在其内侧的内部空间和设置在其任一侧的外部入口/出口门，L形分隔件安装在 用于生产和测试细胞治疗产品的空间，所述隔板具有设置在其任一侧的内入口/出口门，以及放置在隔板内的洁净工作台装置，所述清洁台装置包括第一，第二和第三清洁台 用于防止细胞的污染和最大程度地增加空间的效用。 无尘室和洁净台的运行具有防止污染，提高细胞治疗产品质量和可靠性的优点。

公开(公告)号：US20090137965A1

公开(公告)日：2009-05-28

申请号：US12226316

申请日：2006-06-02

Applicant: Seok-Jung Kim ,  Cheong-Ho Chang ,  Jae-Deog Jang ,  Hyun-Shin Park ,  Sae-Bom Lee ,  Kyoung-Phil Byun

Inventor： Seok-Jung Kim ,  Cheong-Ho Chang ,  Jae-Deog Jang ,  Hyun-Shin Park ,  Sae-Bom Lee ,  Kyoung-Phil Byun

IPC: A61M5/315

CPC classification number: A61B17/8822 ,  A61M5/315

Abstract: An apparatus for operating a syringe piston is developed, which is comprised of: a syringe mounting section for fixedly holding a syringe, a thread hole formed at an upper head part of the syringe mounting section, and a piston press unit having a threaded rod, which is inserted into the thread hole so that the syringe piston press unit can move up and down along the thread hole, a locking slot for fixedly inserting and retaining the medicine filled syringe, a semi-cylindrical tube to assemble with the syringe mounting section. The syringe filled with medical cement is attached to the syringe mounting section and the syringe piston is moved downward with no reverse movement when the piston press unit is rotated, so the medical cement contained in the syringe is injected with a constant pressure, so that a predetermined amount of medical cement is precisely injected into a patient's body.

Abstract translation: 开发了一种用于操作注射器活塞的装置，其包括：用于固定地保持注射器的注射器安装部分，形成在注射器安装部分的上头部的螺纹孔和具有螺杆的活塞压力单元， 其插入到螺纹孔中，使得注射器活塞压力单元可以沿着螺纹孔上下移动，用于固定地插入和保持药液填充的注射器的锁定槽，与注射器安装部分组装的半圆柱形管。 填充有医用水泥的注射器被安装在注射器安装部上，当活塞压力单元旋转时，注射器活塞不会反向移动，所以注射器中所含的医用胶水被注入一定的压力， 将预定量的医用水泥精确地注入患者体内。

公开(公告)号：US20090126285A1

公开(公告)日：2009-05-21

申请号：US12224855

申请日：2006-03-16

Applicant: Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Sung-Jun Koh ,  Eun-Young Lee ,  Soo-Jin Jung ,  Dong-il Chang ,  Jun-Keun Lee ,  Hyun-Gi Yoon ,  Hyang-Soon Chang ,  Yong-Hyun Yoo ,  Jin-Wook Chung ,  Cheong-Ho Chang

Inventor： Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Sung-Jun Koh ,  Eun-Young Lee ,  Soo-Jin Jung ,  Dong-il Chang ,  Jun-Keun Lee ,  Hyun-Gi Yoon ,  Hyang-Soon Chang ,  Yong-Hyun Yoo ,  Jin-Wook Chung ,  Cheong-Ho Chang

IPC: E04H5/00 ,  C12M3/00

CPC classification number: F24F3/0442 ,  C12M23/44 ,  C12M37/00 ,  C12M37/06 ,  C12M41/14 ,  C12M45/22 ,  F24F3/161

Abstract: A facility module is provided for producing and storing a cell therapy product comprising: a Cell Therapy (CT) module one including separately prefabricated units having specific functions and separate entrances and exits so as to minimize contamination, and being capable of producing the cell therapy product, and a Banking of Cell and Tissue (BC) module Two including prefabricated units having specific functions and separate entrances and exits so as to minimize contamination, and being capable of appropriately storing hematopoietic stem cells, bone marrow cells and other cells for a prolonged period. It enables easy and low cost production of the cell therapy product, with sufficient quality to be transplanted into patients, within a short period, and permits clinical application to patients expeditiously. The present invention enables convenient installation and use of such a facility module anywhere adequate space is available, by providing the facility in a prefabricated module composed of specialized units according to function.

Abstract translation: 提供了用于生产和储存细胞治疗产品的设施模块，包括：细胞治疗（CT）模块，其包括具有特定功能的单独预制单元和分开的入口和出口，以使污染最小化，并且能够产生细胞治疗产品 ，以及细胞和组织（BC）模块二，包括具有特定功能的预制单元和单独的入口和出口，以最小化污染，并且能够长时间适当地储存造血干细胞，骨髓细胞和其他细胞 。 它能够在短时间内容易且低成本地生产细胞治疗产品，具有足够的质量移植到患者体内，并允许临床快速应用于患者。 本发明通过在根据功能的专用单元组成的预制模块中提供设备，可以方便地将这种设施模块安装和使用在足够的空间。

公开(公告)号：US07781158B2

公开(公告)日：2010-08-24

申请号：US11885386

申请日：2006-03-09

Applicant: Ji-Chul Yu ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Sae-Bom Lee ,  Se-Geun Yeo ,  Chang-Kwon Ko

Inventor： Ji-Chul Yu ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Sae-Bom Lee ,  Se-Geun Yeo ,  Chang-Kwon Ko

IPC: A01N1/00 ,  C12P21/04 ,  C12P21/06 ,  A61K38/00 ,  A61K38/39 ,  C07K14/00

CPC classification number: C07K14/78

Abstract: A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg/mL.

Abstract translation: 公开了从各种动物组织分离胶原蛋白的方法，用于制备胶原蛋白溶液和使用其的产品。 将猪组织加工成具有适当的形式和尺寸以进行酸处理。 用胃蛋白酶重复酸处理以分离I型或II型胶原蛋白。 分离的胶原蛋白进行盐分处理以进行分级和乙醇处理，从初始组织重量获得5〜10％的胶原蛋白。 处理所制备的组织通过胶原分离过程分离胶原。 处理分离的胶原蛋白以制备产品。 制备产品的方法包括：在低温下在中性条件下处理具有预定浓度的胶原溶液，然后在30至35℃的温度下进行过夜处理; 通过离心浓缩胶原蛋白; 将如此浓缩的胶原蛋白溶解在冷冻的弱酸性溶剂或磷酸盐缓冲盐水（PBS）中，由此制备浓度为1〜5mg / mL的胶原。

公开(公告)号：US20080118947A1

公开(公告)日：2008-05-22

申请号：US11885386

申请日：2006-03-09

Applicant: Ji-Chul Yu ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Sae-Bom Lee ,  Se-Geun Yeo ,  Chang-Kwon Ko

Inventor： Ji-Chul Yu ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Sae-Bom Lee ,  Se-Geun Yeo ,  Chang-Kwon Ko

IPC: C12P21/06 ,  C07K14/78 ,  A61K38/40

CPC classification number: C07K14/78

Abstract: A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg/mL.

Abstract translation: 公开了从各种动物组织分离胶原蛋白的方法，用于制备胶原蛋白溶液和使用其的产品。 将猪组织加工成具有适当的形式和尺寸用于酸处理。 用胃蛋白酶重复酸处理以分离I型或II型胶原蛋白。 分离的胶原蛋白进行盐分处理以进行分级和乙醇处理，从初始组织重量获得5〜10％的胶原蛋白。 处理所制备的组织以通过胶原分离过程分离胶原。 处理分离的胶原蛋白以制备产品。 制备产品的方法包括：在低温下在中性条件下处理具有预定浓度的胶原溶液，然后在30至35℃的温度下进行过夜处理; 通过离心浓缩胶原蛋白; 将如此浓缩的胶原蛋白溶解在冷冻的弱酸性溶剂或磷酸盐缓冲盐水（PBS）中，由此制备浓度为1〜5mg / mL的胶原。

公开(公告)号：US20150141344A1

公开(公告)日：2015-05-21

申请号：US14401704

申请日：2012-06-08

Applicant: Se Ken Yeo ,  Cheong Ho Chang ,  Ji Chul Yoo ,  Dong Sam Suh ,  Jun Keun Lee ,  Sang Hun Woo

Inventor： Se Ken Yeo ,  Cheong Ho Chang ,  Ji Chul Yoo ,  Dong Sam Suh ,  Jun Keun Lee ,  Sang Hun Woo

IPC: A61K38/39 ,  A61M5/31 ,  A61K31/728

CPC classification number: A61K38/39 ,  A61K31/728 ,  A61L27/26 ,  A61L2400/06 ,  A61L2430/06 ,  A61M5/31 ,  A61M2210/02 ,  C08L5/08 ,  C08L89/06

Abstract: The present invention relates to a composition for repairing cartilage tissue, to a method for producing same, and to the use thereof. The present invention provides a composition for repairing cartilage tissue, which is obtained by mixing collagen and hyaluronic acid using a two-way syringe or a mixer, wherein the collagen has a diluted concentration of 5-60 mg/mL excepting water or a physiological phosphate buffer solution, and the hyaluronic acid has a diluted concentration of 5-20 mg/mL excepting water or a physiological phosphate buffer solution.

Abstract translation: 本发明涉及一种用于修复软骨组织的组合物，其制备方法及其用途。 本发明提供了一种用于修复软骨组织的组合物，其通过使用双向注射器或混合器混合胶原和透明质酸获得，其中所述胶原具有5-60mg / mL的稀释浓度，除了水或生理磷酸盐 缓冲溶液，透明质酸除了水或生理磷酸盐缓冲溶液以外，其浓度为5-20mg / mL。

公开(公告)号：US20070087032A1

公开(公告)日：2007-04-19

申请号：US10578719

申请日：2004-12-07

Applicant: Cheong-Ho Chang ,  Chang-Kwon Ko ,  Jae-Deog Jang ,  Eun-Young Lee ,  Jeong-Yong Choi

Inventor： Cheong-Ho Chang ,  Chang-Kwon Ko ,  Jae-Deog Jang ,  Eun-Young Lee ,  Jeong-Yong Choi

IPC: A61F2/00

CPC classification number: A61K35/32 ,  A61F2/30756 ,  A61K35/36 ,  A61K35/39 ,  A61L27/225 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61K2300/00

Abstract: A cartilage therapeutic composition is developed for clinical transplantation into articulatio genu (knee joints) or ankle joints. It has clinical significance for symptomatic cartilage defects of the femoral condyle (medial, lateral, or trochlear) and bone cartilage defects of the talus (anklebone) in human or animal hosts, The cartilage therapeutic composition comprises a mixture of components of chondrocytes isolated and expanded or differentiated from a host such as a human or animal, and thrombin and a fibrinogen matrix containing fibrinogen. An application of the cartilage therapeutic composition is that a mixture of thrombin, chondrocyte components and a fibrinogen matrix is injected into a cartilage defect region followed by solidification therein. It provides rapid healing and effective regeneration of cartilage without surgical operation. It has the merits of safety and simplicity by allowing the use of an arthroscope for transplantation.

Abstract translation: 软骨治疗组合物被开发用于临床移植到关节（膝关节）或踝关节。 对于人或动物宿主中的距骨（踝骨）的股骨髁（内侧，外侧或滑车）和骨软骨缺损的症状性软骨缺损具有临床意义。软骨治疗组合物包含分离和扩大的软骨细胞成分的混合物 或与宿主如人或动物分化，以及含有纤维蛋白原的凝血酶和纤维蛋白原基质。 软骨治疗组合物的应用是将凝血酶，软骨细胞成分和纤维蛋白原基质的混合物注射到软骨缺损区域中，随后在其中固化。 它可以快速愈合，有效地再生软骨，无需外科手术。 通过允许使用关节镜进行移植，具有安全和简单的优点。