公开(公告)号：US08758592B2

公开(公告)日：2014-06-24

申请号：US13550176

申请日：2012-07-16

申请人： Ronald C. Chatelier ,  Dennis Rylatt

发明人： Ronald C. Chatelier ,  Dennis Rylatt

IPC分类号： G01N27/327

CPC分类号： G01N33/54326 ,  G01N33/5438

摘要： An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.

摘要翻译： 提供了用于测量样品（例如血液）中特定成分（例如抗原）的存在或浓度的装置和方法中的粘合剂组合物。 在粘合剂组合物的一个示例性实施方案中，组合物包括粘合剂，水，泊洛沙姆和抗凝血剂。 粘合剂可以包括特定的性质，例如亲水的，压敏的，热活化的和/或水溶性的。 粘合剂是特别有用的，因为它可以帮助改善样品的流动。 例如，当该装置是免疫传感器时，粘合剂可以帮助防止血液在免疫传感器的腔室内凝结。 这样可以更有效和准确地测定样品的浓度。 提供可以使用组合物的组合物和装置的方法，以及使用该组合物的方法。

公开(公告)号：US08221994B2

公开(公告)日：2012-07-17

申请号：US12570268

申请日：2009-09-30

申请人： Ronald C. Chatelier ,  Dennis Rylatt

发明人： Ronald C. Chatelier ,  Dennis Rylatt

IPC分类号： G01N27/327

CPC分类号： G01N33/54326 ,  G01N33/5438

摘要： An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.

摘要翻译： 提供了用于测量样品（例如血液）中特定成分（例如抗原）的存在或浓度的装置和方法中的粘合剂组合物。 在粘合剂组合物的一个示例性实施方案中，组合物包括粘合剂，水，泊洛沙姆和抗凝血剂。 粘合剂可以包括特定的性质，例如亲水的，压敏的，热活化的和/或水溶性的。 粘合剂是特别有用的，因为它可以帮助改善样品的流动。 例如，当该装置是免疫传感器时，粘合剂可以帮助防止血液在免疫传感器的腔室内凝结。 这样可以更有效和准确地测定样品的浓度。 提供可以使用组合物的组合物和装置的方法，以及使用该组合物的方法。

公开(公告)号：US20050167270A1

公开(公告)日：2005-08-04

申请号：US11088797

申请日：2005-03-25

申请人： Gyula Vigh ,  David Ogle ,  Dennis Rylatt

发明人： Gyula Vigh ,  David Ogle ,  Dennis Rylatt

IPC分类号： G01N27/26 ,  B01D57/02 ,  B01D61/46 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  G01N27/447 ,  B01D59/42

CPC分类号： G01N27/44795 ,  B01D57/02 ,  G01N27/44769

摘要： An electrophoretic apparatus comprising: a first electrolyte chamber containing a first electrode; a second electrolyte chamber containing a second electrode; a first sample chamber disposed between the first and second electrolyte chambers and proximate to the first electrolyte chamber; a second sample chamber disposed between the first sample chamber and the second electrolyte; three ion-permeable barriers separating the first electrolyte chamber, the first sample chamber, the second sample chamber, and the second electrolyte chamber, respectively, wherein the ion-permeable barriers impede convective mixing of the contents in each of the respective chambers; a first electrolyte reservoir and a second electrolyte reservoir in fluid communication with the first and second electrolyte chambers, respectively; a first sample reservoir and a second sample reservoir in fluid communication with the first and second sample chambers, respectively; means adapted for communicating a first electrolyte and a second electrolyte between the respective electrolyte chambers and reservoirs; means adapted for communicating a first fluid and a second fluid between the respective sample chambers and reservoirs, wherein at least one of the first and second fluid contains at least a sample, wherein application of an electric potential causes migration of at least one component through at least one of the ion-permeable barriers.

摘要翻译： 一种电泳装置，包括：含有第一电极的第一电解质室; 包含第二电极的第二电解质室; 第一样品室，设置在第一和第二电解质室之间并且靠近第一电解质室; 设置在第一样品室和第二电解质之间的第二样品室; 分别分离第一电解质室，第一样品室，第二样品室和第二电解质室的三个离子可渗透屏障，其中离子可渗透屏障阻止各个室中的内容物的对流混合; 分别与第一和第二电解质室流体连通的第一电解质储存器和第二电解质储存器; 分别与第一和第二样品室流体连通的第一样品储存器和第二样品储存器; 用于在各个电解液室和储存器之间连通第一电解质和第二电解质的装置; 适于在相应样品室和储存器之间传送第一流体和第二流体的装置，其中所述第一和第二流体中的至少一个流体包含至少一个样品，其中施加电势导致至少一种组分的迁移通过 至少一个离子渗透屏障。

公开(公告)号：US08951749B2

公开(公告)日：2015-02-10

申请号：US13003752

申请日：2009-07-10

申请人： Dennis Rylatt ,  Alastair Hodges

发明人： Dennis Rylatt ,  Alastair Hodges

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/537 ,  G01N33/538 ,  G01N33/539 ,  G01N33/541 ,  G01N33/558 ,  G01N33/58 ,  G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  B01L3/00

CPC分类号： G01N33/54366 ,  B01L3/5027 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0694 ,  C12Q1/001 ,  C12Q1/004 ,  C12Q1/005 ,  C12Q1/006 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/53 ,  G01N33/5302 ,  G01N33/5306 ,  G01N33/537 ,  G01N33/538 ,  G01N33/539 ,  G01N33/541 ,  G01N33/54393 ,  G01N33/558 ,  G01N33/581 ,  G01N33/66

摘要： Disclosed herein are devices for detecting the presence of a target analyte in a fluid sample. The biosensor device can comprise at least a reaction chamber and a detection chamber. The device can comprise a amplifying mechanism such that one target analyte molecule present in the fluid sample can lead to generation/activation of multiple detection agent molecules, and therefore, an amplified signal. Also disclosed are the methods of manufacturing and using such a biosensor device.

摘要翻译： 本文公开了用于检测流体样品中目标分析物的存在的装置。 生物传感器装置可以至少包括反应室和检测室。 该装置可以包括放大机制，使得存在于流体样品中的一个目标分析物分子可以导致多个检测剂分子的产生/激活，并因此导致放大的信号。 还公开了制造和使用这种生物传感器装置的方法。

公开(公告)号：US08088265B2

公开(公告)日：2012-01-03

申请号：US10556910

申请日：2004-05-14

申请人： Dennis Rylatt ,  Sharon Leong

发明人： Dennis Rylatt ,  Sharon Leong

IPC分类号： B01D57/02

CPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/44756

摘要： A process for separating a cell type from a mixture of cell types by electrophoresis comprising providing a sample containing a mixture of cell types to a sample chamber of membrane-based electrophoresis apparatus adapted to separate cells and applying an electric potential causing at least one cell type in the sample to be separated from other cells in the sample.

摘要翻译： 一种通过电泳从细胞类型混合物中分离细胞类型的方法，包括提供含有细胞类型混合物的样品至适于分离细胞的膜基电泳装置的样品室，并施加导致至少一种细胞类型的电位 在样品中与样品中的其他细胞分离。

公开(公告)号：US20110073493A1

公开(公告)日：2011-03-31

申请号：US12570268

申请日：2009-09-30

申请人： Ronald C. Chatelier ,  Dennis Rylatt

发明人： Ronald C. Chatelier ,  Dennis Rylatt

IPC分类号： G01N33/50 ,  G01N27/26 ,  C09J171/00

CPC分类号： G01N33/54326 ,  G01N33/5438

摘要： An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.

摘要翻译： 提供了用于测量样品（例如血液）中特定成分（例如抗原）的存在或浓度的装置和方法中的粘合剂组合物。 在粘合剂组合物的一个示例性实施方案中，组合物包括粘合剂，水，泊洛沙姆和抗凝血剂。 粘合剂可以包括特定的性质，例如亲水的，压敏的，热活化的和/或水溶性的。 粘合剂是特别有用的，因为它可以帮助改善样品的流动。 例如，当该装置是免疫传感器时，粘合剂可以帮助防止血液在免疫传感器的腔室内凝结。 这样可以更有效和准确地测定样品的浓度。 提供可以使用组合物的组合物和装置的方法，以及使用该组合物的方法。

公开(公告)号：US20080067070A1

公开(公告)日：2008-03-20

申请号：US10556910

申请日：2004-05-14

申请人： Dennis Rylatt ,  Sharon Leong

发明人： Dennis Rylatt ,  Sharon Leong

IPC分类号： B01D57/02

CPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/44756

摘要： A process for separating a cell type from a mixture of cell types by electrophoresis comprising providing a sample containing a mixture of cell types to a sample chamber of membrane-based electrophoresis apparatus adapted to separate cells and applying an electric potential causing at least one cell type in the sample to be separated from other cells in the sample.

摘要翻译： 一种通过电泳从细胞类型混合物中分离细胞类型的方法，包括提供含有细胞类型混合物的样品至适于分离细胞的膜基电泳装置的样品室，并施加导致至少一种细胞类型的电位 在样品中与样品中的其他细胞分离。

公开(公告)号：US20060134713A1

公开(公告)日：2006-06-22

申请号：US11284097

申请日：2005-11-21

申请人： Dennis Rylatt ,  Alastair Hodges ,  Ronald Chatelier

发明人： Dennis Rylatt ,  Alastair Hodges ,  Ronald Chatelier

IPC分类号： G01N33/537 ,  C12M1/34 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5438 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54326 ,  Y10T29/49124

摘要： Disclosed herein are methods and devices for detecting the presence of an analyte of interest. A biosensor device can include a reaction chamber and an electrochemical detection chamber. The reaction chamber can include at least one immobilized binding site and a probe conjugate adapted to bind to at least one of the target analyte and the immobilized binding site, while the detection chamber can include electrodes for detecting an electrochemical reaction. If present, the target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, which can be detected electrochemically in the detection chamber.

摘要翻译： 本文公开了用于检测感兴趣的分析物的存在的方法和装置。 生物传感器装置可以包括反应室和电化学检测室。 反应室可以包括至少一个固定的结合位点和适于结合至少一个目标分析物和固定的结合位点的探针结合体，而检测室可以包括用于检测电化学反应的电极。 如果存在，则流体样品中的目标分析物导致在反应室中结合的探针结合物的量的变化，其可以在检测室中电化学检测。

公开(公告)号：US20060037860A1

公开(公告)日：2006-02-23

申请号：US11188111

申请日：2005-07-25

申请人： David Ogle ,  Dennis Rylatt

发明人： David Ogle ,  Dennis Rylatt

IPC分类号： G01N27/26

CPC分类号： B01D61/425 ,  B01D57/02 ,  G01N27/44773

摘要： An apparatus for processing compounds in small volumes by electrophoresis, the apparatus comprising: (a) a cathode in a static cathode buffer zone or compartment; (b) an anode in a static anode buffer zone or compartment, the anode disposed relative to the cathode so as to be adapted to generate an electric field in an electric field area therebetween upon application of a voltage potential between the cathode and anode; (c) a first separation barrier disposed in the electric field area; (d) a second separation barrier disposed between a selected one of the cathode buffer zone and the anode buffer zone and the first barrier so as to define a first interstitial volume or chamber therebetween; wherein in use, electrophoretic buffer is disposed in the cathode buffer zone and the anode buffer zone, a sample constituent is provided to the first interstitial volume; wherein upon application of the voltage potential, a selected separation product is removed from the sample constituent through a selected one of the first and second separation barriers, and provided to a selected one of the cathode buffer and anode buffer zones; and wherein there is substantially no circulation of buffer or sample constituent in the buffer zones or the first interstitial volume.

摘要翻译： 一种用于通过电泳处理小体积化合物的装置，所述装置包括：（a）静电阴极缓冲区或隔室中的阴极; （b）静电阳极缓冲区或隔室中的阳极，阳极相对于阴极设置，以便在施加阴极和阳极之间的电压电位时，在其间的电场区域中产生电场; （c）设置在电场区域中的第一分离屏障; （d）设置在阴极缓冲区和阳极缓冲区中的所选择的一个和第一屏障之间的第二隔离屏障，以便在其间限定第一间隙容积或室; 其中在使用中，电泳缓冲液设置在阴极缓冲区和阳极缓冲区中，将样品成分提供给第一间隙体积; 其中在施加电压电势时，通过所选择的第一和第二分离屏障中的一个，将选定的分离产物从样品成分中除去，并提供给选定的一个阴极缓冲区和阳极缓冲区; 并且其中在缓冲区或第一间隙体积中基本上不存在缓冲液或样品成分的循环。