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公开(公告)号：US20120279873A1
公开(公告)日：2012-11-08
申请号：US13550176
申请日：2012-07-16
申请人： Ronald C. Chatelier , Dennis Rylatt
发明人： Ronald C. Chatelier , Dennis Rylatt
IPC分类号： C12Q1/54
CPC分类号： G01N33/54326 , G01N33/5438
摘要： An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.
摘要翻译： 提供了用于测量样品（例如血液）中特定成分（例如抗原）的存在或浓度的装置和方法中的粘合剂组合物。 在粘合剂组合物的一个示例性实施方案中，组合物包括粘合剂，水，泊洛沙姆和抗凝血剂。 粘合剂可以包括特定的性质，例如亲水的，压敏的，热活化的和/或水溶性的。 粘合剂是特别有用的，因为它可以帮助改善样品的流动。 例如，当该装置是免疫传感器时，粘合剂可以帮助防止血液在免疫传感器的腔室内凝结。 这样可以更有效和准确地测定样品的浓度。 提供可以使用组合物的组合物和装置的方法，以及使用该组合物的方法。
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公开(公告)号：US20120090998A1
公开(公告)日：2012-04-19
申请号：US13316006
申请日：2011-12-09
申请人： Dennis Rylatt , Sharon Leong
发明人： Dennis Rylatt , Sharon Leong
IPC分类号： G01N33/559 , G01N27/26
CPC分类号： B01D57/02 , G01N27/447 , G01N27/44756
摘要： A process for separating a cell type from a mixture of cell types by electrophoresis comprising providing a sample containing a mixture of cell types to a sample chamber of a membrane-based electrophoresis apparatus adapted to separate cells and applying an electric potential causing at least one cell type in the sample to be separated from other cells in the sample.
摘要翻译： 一种通过电泳从细胞类型混合物中分离细胞类型的方法，其包括将含有细胞类型混合物的样品提供到适于分离细胞并施加导致至少一个细胞的电势的基于膜的电泳装置的样品室 键入要与样品中其他细胞分离的样品。
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公开(公告)号：US20110111425A1
公开(公告)日：2011-05-12
申请号：US13003752
申请日：2009-07-10
申请人： Dennis Rylatt , Alastair Hodges
发明人： Dennis Rylatt , Alastair Hodges
CPC分类号： G01N33/54366 , B01L3/5027 , B01L2200/0621 , B01L2200/10 , B01L2300/0645 , B01L2300/0825 , B01L2300/087 , B01L2400/0406 , B01L2400/0694 , C12Q1/001 , C12Q1/004 , C12Q1/005 , C12Q1/006 , C12Q1/26 , C12Q1/32 , G01N33/53 , G01N33/5302 , G01N33/5306 , G01N33/537 , G01N33/538 , G01N33/539 , G01N33/541 , G01N33/54393 , G01N33/558 , G01N33/581 , G01N33/66
摘要： Disclosed herein are devices for detecting the presence of a target analyte in a fluid sample. The biosensor device can comprise at least a reaction chamber and a detection chamber. The device can comprise a amplifying mechanism such that one target analyte molecule present in the fluid sample can lead to generation/activation of multiple detection agent molecules, and therefore, an amplified signal. Also disclosed are the methods of manufacturing and using such a biosensor device.
摘要翻译： 本文公开了用于检测流体样品中目标分析物的存在的装置。 生物传感器装置可以至少包括反应室和检测室。 该装置可以包括放大机制，使得存在于流体样品中的一个目标分析物分子可以导致多个检测剂分子的产生/激活，并因此导致放大的信号。 还公开了制造和使用这种生物传感器装置的方法。
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公开(公告)号：US09863942B2
公开(公告)日：2018-01-09
申请号：US12563091
申请日：2009-09-18
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/5438 , G01N33/53 , G01N33/543 , G01N33/54326 , Y10T29/49124
摘要： Disclosed herein are methods and devices for detecting the presence of an analyte of interest. A biosensor device can include a reaction chamber and an electrochemical detection chamber. The reaction chamber can include at least one immobilized binding site and a probe conjugate adapted to bind to at least one of the target analyte and the immobilized binding site while the detection chamber can include electrodes for detecting an electrochemical reaction. If present, the target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, which can be detected electrochemically in the detection chamber.
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公开(公告)号：US20120283358A1
公开(公告)日：2012-11-08
申请号：US13550114
申请日：2012-07-16
申请人： Ronald C. Chatelier , Dennis Rylatt
发明人： Ronald C. Chatelier , Dennis Rylatt
IPC分类号： C09J163/00 , C08K5/11 , C08K5/092 , C09J105/10
CPC分类号： G01N33/54326 , G01N33/5438
摘要： An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.
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公开(公告)号：US06969453B2
公开(公告)日：2005-11-29
申请号：US09837678
申请日：2001-04-18
申请人： David Ogle , Dennis Rylatt
发明人： David Ogle , Dennis Rylatt
IPC分类号： G01N1/10 , B01D57/02 , B01D61/18 , B01D61/28 , B01D61/42 , B01D61/46 , B01D61/58 , B01D71/40 , B03C5/00 , C07K1/26 , C25B7/00 , G01N27/26 , G01N27/447 , G01N7/00 , G01N27/453
CPC分类号： B01D61/425 , B01D57/02 , G01N27/44773
摘要： An electrophoresis apparatus for processing compounds in small sample volumes comprising a cathode in a static cathode buffer zone or compartment, an anode in a static anode buffer zone or compartment. The cathode disposed relative to the anode so as to be adapted to generate an electric field in an electric field area therebetween upon application of a voltage potential between the cathode and anode. A removable cartridge disposed in the electric field area between the anode and the cathode. The cartridge containing a first non-isoelectric separation barrier and also a second non-isoelectric separation barrier disposed between a selected one of the cathode buffer zone and the anode buffer zone and the first barrier so as to define a first chamber having an interstitial volume of less than 5 ml therebetween.
摘要翻译： 用于处理小样品体积的化合物的电泳装置，包括静电阴极缓冲区或隔室中的阴极，静态阳极缓冲区或隔室中的阳极。 阴极相对于阳极设置，以适于在施加阴极和阳极之间的电压电位时在其间的电场区域中产生电场。 设置在阳极和阴极之间的电场区域中的可拆卸的盒。 所述盒包含第一非等电分离屏障，以及设置在所选阴极缓冲区和阳极缓冲区之间的第二非等电分离屏障和第一屏障，以便限定具有间隙体积的第一室 小于5毫升。
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公开(公告)号：US20050199498A1
公开(公告)日：2005-09-15
申请号：US09787368
申请日：2001-03-14
申请人： Chenicheri Nair , Dennis Rylatt , Andrew Gilbert
发明人： Chenicheri Nair , Dennis Rylatt , Andrew Gilbert
摘要： A method of separating a blood clotting protein from a mixture of blood clotting protein and at least one contaminant, the method comprising: (a) placing a blood clotting protein and contaminant mixture in a first solvent stream, the first solvent stream being separated from a second solvent stream by a first electrophoretic membrane; (b) selecting a buffer for the first solvent stream being a pH greater than the isoelectric point of the blood clotting protein; (c) applying an electric potential between the first and second solvent streams causing movement of at least some of the contaminants through the membrane into the second solvent stream while the blood clotting protein is substantially retained in the first solvent stream, or if entering the membrane, being substantially prevented from entering the second solvent stream; (d) optionally periodically stopping and reversing the electric potential to cause movement of any blood clotting protein having entered the membrane to move back into the first solvent stream, wherein substantially not causing any contaminants that have entered the second solvent stream to re-enter first solvent stream; and (e) maintaining step (c) until the first solvent stream contains the desired purity of blood clotting protein substantially mimicking the characteristics of natural blood clotting protein.
摘要翻译： 一种从血液凝固蛋白和至少一种污染物的混合物中分离凝血蛋白的方法，所述方法包括：（a）将血液凝固蛋白和污染物混合物置于第一溶剂流中，将第一溶剂流从 第二溶剂流通过第一电泳膜; （b）选择第一溶剂流的缓冲液，其pH值大于血液凝固蛋白质的等电点; （c）在第一和第二溶剂流之间施加电位，引起至少一些污染物通过膜移动到第二溶剂流中，同时血液凝固蛋白基本上保留在第一溶剂流中，或者如果进入膜 基本上防止进入第二溶剂流; （d）任选地周期性地停止和反转电位以引起已经进入膜的任何血液凝固蛋白的移动回到第一溶剂流中，其中基本上不会使进入第二溶剂流的任何污染物重新进入第一溶剂流 溶剂流; 和（e）保持步骤（c），直到第一溶剂流含有基本上模拟天然凝血蛋白特征的所需纯度的凝血蛋白。
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8.发明授权Systems, devices, and methods for measuring whole blood hematocrit based on initial fill velocity 有权基于初始填充速度测量全血血细胞比容的系统，装置和方法公开(公告)号：US08877034B2
公开(公告)日：2014-11-04
申请号：US12649509
申请日：2009-12-30
CPC分类号： G01N27/3272 , G01N27/3273 , G01N27/3274 , G01N27/48 , G01N33/49 , G01N33/80 , G01N2333/4737
摘要： Methods for determining the hematocrit of a blood sample, and devices and systems used in conjunction with the same. The hematocrit value can be determined on its own, and further, it can be further used to determine a concentration of an analyte in a sample. In one exemplary embodiment of a method for determining the hematocrit value in a blood sample, a volume of blood is provided in a sample analyzing device having a working and a counter electrode. An electric potential is applied between the electrodes and an initial fill velocity of the sample into the device is calculated. The hematocrit of the blood, as well as a concentration of an analyte in view of the initial fill velocity can then be determined. Systems and devices that take advantage of the use of an initial fill velocity to determine hematocrit levels and make analyte concentration determinations are also provided.
摘要翻译： 用于确定血液样本的血细胞比容的方法，以及与之一起使用的装置和系统。 血细胞比容值可以单独测定，而且还可以用于确定样品中分析物的浓度。 在用于确定血液样品中的血细胞比容值的方法的一个示例性实施方案中，在具有工作和对电极的样品分析装置中提供体积的血液。 在电极之间施加电位，并计算样品进入器件的初始填充速度。 然后可以确定血液的血细胞比容，以及考虑到初始填充速度的分析物的浓度。 还提供利用初始填充速度来确定血细胞比容水平并进行分析物浓度测定的系统和装置。
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9.发明申请Systems, Devices, and Methods for Measuring Whole Blood Hematocrit Based on Initial Fill Velocity 有权基于初始填充速度测量全血血细胞比容的系统，设备和方法公开(公告)号：US20110155584A1
公开(公告)日：2011-06-30
申请号：US12649509
申请日：2009-12-30
IPC分类号： G01N27/26
CPC分类号： G01N27/3272 , G01N27/3273 , G01N27/3274 , G01N27/48 , G01N33/49 , G01N33/80 , G01N2333/4737
摘要： Methods for determining the hematocrit of a blood sample, and devices and systems used in conjunction with the same. The hematocrit value can be determined on its own, and further, it can be further used to determine a concentration of an analyte in a sample. In one exemplary embodiment of a method for determining the hematocrit value in a blood sample, a volume of blood is provided in a sample analyzing device having a working and a counter electrode. An electric potential is applied between the electrodes and an initial fill velocity of the sample into the device is calculated. The hematocrit of the blood, as well as a concentration of an analyte in view of the initial fill velocity can then be determined. Systems and devices that take advantage of the use of an initial fill velocity to determine hematocrit levels and make analyte concentration determinations are also provided.
摘要翻译： 用于确定血液样本的血细胞比容的方法，以及与之一起使用的装置和系统。 血细胞比容值可以单独测定，而且还可以用于确定样品中分析物的浓度。 在用于确定血液样品中的血细胞比容值的方法的一个示例性实施方案中，在具有工作和对电极的样品分析装置中提供体积的血液。 在电极之间施加电位，并计算样品进入器件的初始填充速度。 然后可以确定血液的血细胞比容，以及考虑到初始填充速度的分析物的浓度。 还提供利用初始填充速度来确定血细胞比容水平并进行分析物浓度测定的系统和装置。
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公开(公告)号：US20100006452A1
公开(公告)日：2010-01-14
申请号：US12563091
申请日：2009-09-18
CPC分类号： G01N33/5438 , G01N33/53 , G01N33/543 , G01N33/54326 , Y10T29/49124
摘要： Disclosed herein are methods and devices for detecting the presence of an analyte of interest. A biosensor device can include a reaction chamber and an electrochemical detection chamber. The reaction chamber can include at least one immobilized binding site and a probe conjugate adapted to bind to at least one of the target analyte and the immobilized binding site while the detection chamber can include electrodes for detecting an electrochemical reaction. If present, the target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, which can be detected electrochemically in the detection chamber.
摘要翻译： 本文公开了用于检测感兴趣的分析物的存在的方法和装置。 生物传感器装置可以包括反应室和电化学检测室。 反应室可以包括至少一个固定的结合位点和适于结合至少一种目标分析物和固定的结合位点的探针共轭体，同时检测室可以包括用于检测电化学反应的电极。 如果存在，则流体样品中的目标分析物导致在反应室中结合的探针结合物的量的变化，其可以在检测室中电化学检测。
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