公开(公告)号：US08679827B2

公开(公告)日：2014-03-25

申请号：US13495224

申请日：2012-06-13

申请人： Graham Davis ,  Imants Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： C12M3/00

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to methods for measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit. A solution comprising a substrate for said enzyme and at least one air segment contacts the sensor to remove unbound analyte and labeled antibody from a region of the sensor. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及用于在包括传感器的导管中使用电化学测定来测量分析物的量的方法，其中所述传感器包括具有结合所述分析物的固定化抗体表面层的电极，以及设置在 说导管。 包含用于所述酶的底物和至少一个空气区段的溶液与传感器接触以从传感器的区域去除未结合的分析物和标记的抗体。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

公开(公告)号：US08222024B2

公开(公告)日：2012-07-17

申请号：US13198253

申请日：2011-08-04

申请人： Graham Davis ,  Imants Lauks ,  Chao Lin ,  Cary James Miller

发明人： Graham Davis ,  Imants Lauks ,  Chao Lin ,  Cary James Miller

IPC分类号： C12M3/00

CPC分类号： G01N33/543 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0683 ,  G01N33/54366 ,  G01N35/08 ,  G01N2035/0436

摘要： The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

摘要翻译： 本发明涉及一种用于进行各种测定液体样品中分析物测定的装置，并涉及这种测定方法。 特别地，本发明涉及一种设计成能够适用于各种实时测定方案的单次使用的盒，优选使用免疫传感器或其他基于配体/配体受体的生物传感器实施方案来测定生物样品中分析物的测定。 该盒提供用于处理样品的计量部分的新颖特征，用于精确和灵活地控制盒内样品或第二流体的运动，用于在测定过程中用附加化合物修饰溶液以及用于构建免疫传感器 能够适应不同的分析物测量。 所公开的装置和使用方法相对于现有技术具有实质性的益处，包括操作者的简单使用，一种或多种分析物的快速原位测定，以及使操作者和患者的污染风险最小化的一次性方法。 所公开的发明适用于临床诊断领域，包括在事故现场，急诊室，手术室，疗养院，重症监护室和非医疗环境中的使用。

公开(公告)号：US20100202926A1

公开(公告)日：2010-08-12

申请号：US12718139

申请日：2010-03-05

申请人： Imants LAUKS ,  Raymond J. PIERCE ,  James WOJTYK ,  Benoit R. BERGEVIN

发明人： Imants LAUKS ,  Raymond J. PIERCE ,  James WOJTYK ,  Benoit R. BERGEVIN

IPC分类号： G01N27/00 ,  F04F99/00

CPC分类号： B01L3/50273 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0418 ,  F04B19/006 ,  Y10T436/2575

摘要： Devices with lateral flow elements and integral fluidics are disclosed. The integral fluidics consist of injector pumps comprised of fluidic elements under instrument control. The fluidic element of an injector pump is fluidically connected to lateral flow elements and can be used to control fluid entry into containment chambers referred to as micro-reactors. The lateral flow elements comprise conductor elements that can be used for sample application and transport of analyte contained in the sample to the micro-reactor. Fluidic transport through the fluidic element of the injector pump is under instrument-control. Both the lateral flow element and the fluidic element may contain chemical entities incorporated along their length. The chemical reactions that can be used for analyte detection using the devices are described. Also described are methods of manufacture of these devices.

摘要翻译： 公开了具有侧向流动元件和整体流体的装置。 积分流体由在仪器控制下由流体元素组成的喷射泵组成。 注射器泵的流体元件流体连接到侧向流动元件，并且可以用于控制流体进入称为微反应器的容纳室中。 横向流动元件包括导体元件，其可以用于将样品中包含的分析物的样品应用和运送到微反应器。 通过注射泵的流体元件的流体传输是在仪器控制下。 侧流元件和流体元件都可以包含沿其长度并入的化学物质。 描述了可用于使用装置进行分析物检测的化学反应。 还描述了这些装置的制造方法。

公开(公告)号：US20070170062A1

公开(公告)日：2007-07-26

申请号：US11697116

申请日：2007-04-05

申请人： Imants LAUKS

发明人： Imants LAUKS

IPC分类号： G01N27/00

CPC分类号： B01L3/50273 ,  B01J2219/00783 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/10 ,  B01L2400/0415 ,  B01L2400/0418 ,  G01N2035/00158

摘要： Devices with electrokinetic elements are disclosed as well as their method of microfabrication for use in micro-scale analysis, mixture separation and reaction. The devices consist of solid hydrophilic-matrix films that have been microfabricated into a variety of micro-scale structures. These structures include hydrophilic-matrix conductors for electrokinetic species transport and separation. They also include hydrophilic-matrix cladding containing chemical species adjacent to either an open conduit or a hydrophilic matrix conductor. Also described are other integrated microstructures consisting of hydrophilic-matrix materials such as micro-reaction zones for retaining chemical species for on-chip chemical reactions and integrated detection structures for on-chip species detection. In general, a hydrophilic matrix on a substrate functions as a conductor that is covered by an electrically insulating, preferably water permeable material.

摘要翻译： 公开了具有电动元件的装置以及其用于微尺度分析，混合物分离和反应的微细加工方法。 这些装置由已经微加工成各种微尺度结构的固体亲水基质膜组成。 这些结构包括用于电动物质输送和分离的亲水基质导体。 它们还包括含有与开放导管或亲水基质导体相邻的化学物质的亲水基质包层。 还描述了由亲水基质材料如用于保留用于片上化学反应的化学物质的微反应区和用于片上物种检测的集成检测结构组成的其它集成微结构。 通常，衬底上的亲水基质用作被电绝缘，优选水可渗透材料覆盖的导体。

公开(公告)号：US07094330B2

公开(公告)日：2006-08-22

申请号：US10307481

申请日：2002-12-02

申请人： Imants Lauks ,  Andrzej Maczuszenko

发明人： Imants Lauks ,  Andrzej Maczuszenko

IPC分类号： G01N27/403

CPC分类号： B01L3/502715 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/148 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/0683 ,  G01N27/301

摘要： The present invention relates to planar electrochemical sensors with membrane coatings used to perform chemical analyses. The object of this invention is to provide unit-use disposable sensors of very simple and economical construction, preferably with only a single membrane coating on an electrode. The invented devices are potentiometric salt-bridge reference electrodes constructed with a heterogeneous membrane coating of a conductor. The heterogeneous membrane is a formulation that concurrently supports non-volatile species transport through an electrolyte compartment and gas transport through a hydrophobic compartment.

摘要翻译： 本发明涉及具有用于进行化学分析的膜涂层的平面电化学传感器。 本发明的目的是提供非常简单和经济的结构的单元使用一次性传感器，优选地在电极上仅具有单个膜涂层。 本发明的器件是由导体的非均相膜涂层构成的电位盐基参比电极。 非均相膜是同时支持非挥发性物质通过电解质隔室传输并通过疏水隔室输送气体的制剂。

公开(公告)号：US20050150761A1

公开(公告)日：2005-07-14

申请号：US11041453

申请日：2005-01-25

申请人： Imants Lauks

发明人： Imants Lauks

IPC分类号： G01N27/27 ,  G01N27/30 ,  G01N27/327 ,  G01N27/403 ,  G01N27/414 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/307 ,  G01N27/403 ,  H01L2224/48091 ,  H01L2924/3011 ,  H01L2924/00014 ,  H01L2924/00

摘要： A chip-carrier module known from smart card technology is adapted as an electrode array for use in disposable sensing or separation devices containing electrodes. The electrodes are manufactured directly onto chip-carrier module. The preferred electrode module includes a chip-carrier module and at least one electrode formed thereon, the chip-carrier module being a laminate of a metal foil and an insulator foil, which metal foil is divided into at least two conductor regions, the insulator foil having a perforation over each conductor region, and the at least one electrode being formed by a membrane layer applied to the insulating foil to extend through one of the perforations for electrical contact with the conductor region associated therewith, the membrane layer imparting chemical sensitivity to the electrode. The result is an electrode module for use in disposable sensing or separation devices containing electrodes, whose unit cost of manufacture is low even at modest manufacturing volume.

摘要翻译： 从智能卡技术已知的芯片载体模块被适配为用于包含电极的一次性感测或分离装置中的电极阵列。 电极直接制造到芯片载体模块上。 优选的电极模块包括芯片载体模块和形成在其上的至少一个电极，芯片载体模块是金属箔和绝缘体箔的叠层，该金属箔被分成至少两个导体区域，绝缘体箔 在每个导体区域上具有穿孔，并且所述至少一个电极由施加到绝缘箔上的膜层形成，以延伸通过其中一个穿孔以与与其相关联的导体区域电接触，所述膜层对化学敏感性 电极。 结果是用于包含电极的一次性感测或分离装置的电极模块，即使在适度的制造体积下，制造的单位成本也低。