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公开(公告)号：US5969108A
公开(公告)日：1999-10-19
申请号：US971857
申请日：1993-01-08
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C12N1/21 , C07H21/00 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/46 , C07K19/00 , C12N7/00 , C12N7/01 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/13 , C12N15/62 , C12N15/73 , C12P21/02 , C12R1/19 , C40B40/02 , G01N33/53 , G01N33/531 , G01N33/68 , C07H21/04
CPC分类号： C07K16/005 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , B01J2219/00718 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： PCT No.PCT / GB91 / 01134 Sec。 371日期：1993年1月8日 102（e）日期1993年1月8日PCT 1991年7月10日PCT公布。 出版物WO92 / 01047 日期1992年1月23日通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定特异性结合对（sbp）的成员，其中sbp成员以分泌重组体的表面的功能形式显示 遗传显示包（rgdp），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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12.发明授权Production of anti-self antibodies from antibody segment repertoires and displayed on phage 失效从抗体片段制备抗自身抗体并在噬菌体上显示公开(公告)号：US07195866B2
公开(公告)日：2007-03-27
申请号：US10326495
申请日：2002-12-19
申请人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
发明人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/26 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/3092 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/4241 , C07K16/44 , C07K16/462 , C07K16/468 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , Y10S530/867
摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.
摘要翻译： 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所使用的核酸文库可以来源于未免疫的哺乳动物的重新排列的V基因序列。 描述和显示合成或人造文库是有用的。
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公开(公告)号：US07063943B1
公开(公告)日：2006-06-20
申请号：US09196673
申请日：1998-11-20
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , C12N15/1037
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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14.发明授权Production of anti-self antibodies from antibody segment repertoires and displayed on phage 失效从抗体片段制备抗自身抗体并在噬菌体上显示公开(公告)号：US06555313B1
公开(公告)日：2003-04-29
申请号：US09572392
申请日：2000-05-16
申请人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
发明人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
IPC分类号： C12Q170
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/26 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/3092 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/4241 , C07K16/44 , C07K16/462 , C07K16/468 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , Y10S530/867
摘要： Methods are disclosed for the production of human self-antibodies and antibody fragments, which bind human antigens. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Human antibodies or antibody fragments are selected by binding with human antigens. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding to and is a human antigen. Nucleic acid libraries used may be derived from V-gene sequences of unimmunised humans. Part or all of the nucleic acid may be derived from oligonucleotide synthesis.
摘要翻译： 公开了用于生产结合人抗原的人自身抗体和抗体片段的方法。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其表面上显示作为抗体或抗体片段的特异性结合对的成员，以及每个含rgdp的核酸序列衍生自 哺乳动物的种类 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与人抗原结合来选择人抗体或抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合并且是人抗原能力的任何其它片段。 所用的核酸文库可以衍生自未免疫的人的V-基因序列。 部分或全部核酸可以衍生自寡核苷酸合成。
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15.发明授权Production of anti-self antibodies from antibody segment repertories and displayed on phage 失效从抗体片段生产抗自身抗体并显示在噬菌体上公开(公告)号：US06544731B1
公开(公告)日：2003-04-08
申请号：US09197221
申请日：1998-11-20
申请人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
发明人： Andrew David Griffiths , Hendricus Renerus Jacobus Mattheus Hoogenboom , James David Marks , John McCafferty , Gregory Paul Winter , Geoffrey Walter Grigg
IPC分类号： C12N700
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/26 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/3092 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/4241 , C07K16/44 , C07K16/462 , C07K16/468 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , Y10S530/867
摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.
摘要翻译： 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其表面上显示作为抗体或抗体片段的特异性结合对的成员，以及每个含rgdp的核酸序列衍生自 哺乳动物的种类 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所使用的核酸文库可以来源于未免疫的哺乳动物的重新排列的V基因序列。 描述和显示合成或人造文库是有用的。
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公开(公告)号：US20100136660A1
公开(公告)日：2010-06-03
申请号：US12648269
申请日：2009-12-28
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
IPC分类号： C12N7/01
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US07662557B2
公开(公告)日：2010-02-16
申请号：US10803653
申请日：2004-03-18
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。
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公开(公告)号：US07635666B1
公开(公告)日：2009-12-22
申请号：US09416902
申请日：1999-10-13
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Hendricus Reneus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。
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公开(公告)号：US06492123B1
公开(公告)日：2002-12-10
申请号：US09146979
申请日：1998-09-03
申请人： Kaspar-Philip Holliger , Andrew David Griffiths , Hendricus Renerus Jacobus Matheus Hoogenboom , Magnus Malmqvist , James David Marks , Brian Timothy McGuinness , Anthony Richard Pope , Terence Derek Prospero , Gregory Paul Winter
发明人： Kaspar-Philip Holliger , Andrew David Griffiths , Hendricus Renerus Jacobus Matheus Hoogenboom , Magnus Malmqvist , James David Marks , Brian Timothy McGuinness , Anthony Richard Pope , Terence Derek Prospero , Gregory Paul Winter
IPC分类号： G01N3353
CPC分类号： C12N15/113 , A61K38/00 , C07K16/00 , C07K16/1063 , C07K16/241 , C07K16/26 , C07K16/2809 , C07K16/283 , C07K16/3007 , C07K16/3061 , C07K16/40 , C07K16/4283 , C07K16/44 , C07K16/468 , C07K2317/24 , C07K2317/34 , C07K2317/624 , C07K2317/626 , C07K2317/73 , C07K2319/00
摘要： Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.
摘要翻译： 包含第一结构域的多肽，其包含免疫球蛋白重链可变区的结合区和第二结构域，其包含免疫球蛋白轻链可变区的结合区，所述结构域被连接但不能彼此缔合形成 抗原结合位点，缔合以形成抗原结合多聚体，例如二聚体，其可以是多价的或具有多特异性。 结构域可以通过短肽接头连接或者可以直接连接在一起。 双特异性二聚体可以具有更长的接头。 公开了多肽和多聚体及其多种组成成分的制备方法，以及它们在噬菌体表面上的显示以便于选择感兴趣的粘合剂，以及许多实用性。
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公开(公告)号：US07732377B2
公开(公告)日：2010-06-08
申请号：US10803622
申请日：2004-03-18
申请人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
发明人： John McCafferty , Anthony Richard Pope , Kevin Stuart Johnson , Henricus Renerus Jacobus Mattheus Hoogenboom , Andrew David Griffiths , Ronald Henry Jackson , Kaspar Philipp Holliger , James David Marks , Timothy Piers Clackson , David John Chiswell , Gregory Paul Winter , Timothy Peter Bonnert
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a filamentous bacteriophage particle containing DNA encoding the sbp member, wherein the sbp member has a binding domain that consists of a dAb fragment. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected filamentous bacteriophage particles for expression of the selected sbp members.
摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在含有编码DNA的DNA的丝状噬菌体颗粒的表面 sbp成员，其中sbp成员具有由dAb片段组成的结合结构域。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选择的丝状噬菌体颗粒中回收DNA以表达所选择的sbp成员。
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