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31 条记录 (耗时 0.017 秒)

    Novel carbonyl reductase, gene thereof and use of the same
    11.
    发明申请
    Novel carbonyl reductase, gene thereof and use of the same 有权
    新型羰基还原酶，其基因及其用途

    公开(公告)号：US20060046289A1

    公开(公告)日：2006-03-02

    申请号：US10512908

    申请日：2003-04-30

    申请人： Noriyuki Kizaki ,  Miho Horikawa ,  Yoshihiko Yasohara

    发明人： Noriyuki Kizaki ,  Miho Horikawa ,  Yoshihiko Yasohara

    IPC分类号： C12P7/22 ,  C07H21/04 ,  C12P21/06 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12Y101/01184

    摘要： The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3′-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on 2-chloro-1-(3′-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to form (R)-2-chloro-1-(31-chlorophenyl)ethanol; (2) optimum pH for activity: 5.0 to 6.0; (3) optimum temperature for activity: 40° C. to 50° C.; (4) molecular weight: about 40,000 as determined by gel filtration analysis, about 30,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.

    摘要翻译： 本发明提供形成（R）-2-氯-1-（3'-氯苯基）乙醇的新型多肽，编码所述多肽的多核苷酸及其用途。 本发明涉及具有以下物理和化学性质（1）至（4）的多肽：（1）活性：以NADPH或NADH作为辅酶作用于2-氯-1-（3'-氯苯基）乙酮， 形成（R）-2-氯-1-（31-氯苯基）乙醇; （2）活性最佳pH：5.0〜6.0; （3）最适温度为40℃〜50℃。 （4）分子量：约40,000，通过凝胶过滤分析测定，约30,000，通过SDS聚丙烯酰胺凝胶电泳分析测定。 本发明还涉及包含序列表中SEQ ID NO：1所示氨基酸序列的多肽，编码所述多肽的多核苷酸，以及以高水平产生所述多肽的转化体。

    (R)-transaminase from arthrobacter
    12.
    发明授权
    (R)-transaminase from arthrobacter 失效
    （R） - 转氨酶

    公开(公告)号：US06344351B2

    公开(公告)日：2002-02-05

    申请号：US09794359

    申请日：2001-02-28

    申请人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

    发明人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

    IPC分类号： C12N910

    CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

    摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.

    摘要翻译： 制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法， 和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。 根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

    ARYLACYLAMIDASE GENE AND METHOD OF USING THE SAME
    14.
    发明申请
    ARYLACYLAMIDASE GENE AND METHOD OF USING THE SAME 审中-公开
    ARYLACYLAMIDASE基因及其使用方法

    公开(公告)号：US20110236955A1

    公开(公告)日：2011-09-29

    申请号：US11576560

    申请日：2005-09-26

    申请人： Noriyuki Kizaki ,  Noriyuki Ito ,  Yoshihiko Yasohara ,  Toru Nagasawa

    发明人： Noriyuki Kizaki ,  Noriyuki Ito ,  Yoshihiko Yasohara ,  Toru Nagasawa

    IPC分类号： C12N9/80 ,  C12N15/55 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/80

    摘要： The present invention provides an efficient method of producing an arylacylamidase. In an aspect, the present invention provides (a) a DNA comprising the base sequence shown in the sequence listing under SEQ ID NO:1, or (b) a DNA capable of hybridizing, under stringent conditions, with a DNA comprising the base sequence complementary to the base sequence shown in the sequence listing under SEQ ID NO:1 and coding for a polypeptide having arylacylamidase activity. In another aspect, the invention provides a polypeptide encoded by the DNA mentioned above and having arylacylamidase activity, a vector containing the DNA mentioned above, a transformant which is transformed using the DNA or the vector mentioned above, and a method for producing an arylacylamidase which comprises cultivating the transformant mentioned above and obtaining the arylacylamidase from the culture medium.

    摘要翻译： 本发明提供了生产芳基酰胺酶的有效方法。 一方面，本发明提供（a）包含SEQ ID NO：1中的序列表中所示的碱基序列的DNA，或（b）能够在严格条件下与包含碱基序列的DNA杂交的DNA 与SEQ ID NO：1中的序列表中所示的碱基序列互补，并编码具有芳基酰胺酶活性的多肽。 另一方面，本发明提供由上述DNA编码并具有芳基酰胺酶活性的多肽，含有上述DNA的载体，使用上述DNA或载体转化的转化体及其制备方法， 包括培养上述转化体并从培养基中获得芳酰胺酰胺酶。

    METHOD FOR PRODUCING OPTICALLY ACTIVE 2-(N-SUBSTITUTED AMINOMETHYL)-3-HYDROXYBUTYRIC ACID ESTER
    16.
    发明申请
    METHOD FOR PRODUCING OPTICALLY ACTIVE 2-(N-SUBSTITUTED AMINOMETHYL)-3-HYDROXYBUTYRIC ACID ESTER 审中-公开
    生产光学活性2-（N-取代氨基甲酰基）-3-羟基丁酸的方法

    公开(公告)号：US20090104671A1

    公开(公告)日：2009-04-23

    申请号：US11996271

    申请日：2006-07-19

    申请人： Yoshihiko Yasohara ,  Miho Yano ,  Shigeru Kawano ,  Noriyuki Kizaki

    发明人： Yoshihiko Yasohara ,  Miho Yano ,  Shigeru Kawano ,  Noriyuki Kizaki

    IPC分类号： C12P13/02 ,  C12P17/10 ,  C12P13/00

    CPC分类号： C12P13/02 ,  C12P17/10

    摘要： The present invention relates to a method for producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters wherein a 2-(N-substituted aminomethyl)-3-oxobutyric acid ester is treated with an enzyme source capable of stereoselectively reducing said ester to the corresponding optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid ester having the (2S,3R) configuration. The present invention provides an efficient method for industrially producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters, in particular such compounds having the (2S,3R) configuration, which are useful as intermediates for the production of medicinal compounds, among others.

    摘要翻译： 本发明涉及一种光学活性2-（N-取代氨基甲基）-3-羟基丁酸酯的制备方法，其中2-（N-取代氨基甲基）-3-氧代丁酸酯可用立体选择性的酶源处理 将所述酯还原成具有（2S，3R）构型的相应的光学活性2-（N-取代氨基甲基）-3-羟基丁酸酯。 本发明提供了工业上制造光学活性2-（N-取代氨基甲基）-3-羟基丁酸酯的有效方法，特别是具有（2S，3R）构型的这种化合物，其可用作生产药物的中间体 化合物等。

    NOVEL CARBONYL REDUCTASE, GENE THEREFOR AND USE THEREOF
    19.
    发明申请
    NOVEL CARBONYL REDUCTASE, GENE THEREFOR AND USE THEREOF 审中-公开
    新型碳水化合物，其基因及其用途

    公开(公告)号：US20130149769A1

    公开(公告)日：2013-06-13

    申请号：US13093610

    申请日：2011-04-25

    申请人： Noriyuki KIZAKI ,  Makoto UEDA ,  Daisuke MORIYAMA ,  Naoaki TAOKA ,  Yoshihiko YASOHARA

    发明人： Noriyuki KIZAKI ,  Makoto UEDA ,  Daisuke MORIYAMA ,  Naoaki TAOKA ,  Yoshihiko YASOHARA

    IPC分类号： C12N9/00

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

    摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to product (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

    摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离到3-（3-羟基-3-苯基丙腈）产物的3-氧代-3-苯基丙腈的活性的多肽，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。