公开(公告)号：US20050161335A1

公开(公告)日：2005-07-28

申请号：US10500180

申请日：2002-11-29

Applicant: Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

Inventor： Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44791

Abstract: The present invention provides a capillary electrophoresis chip apparatus for detecting nucleotide polymorphism or single nucleotide polymorphism belonging to capillary electrophoresis apparatuses. The apparatus comprises an upper channel layer comprising a one-, two-, or multi-dimensional microfluid channel and an electrode aperture structure for loading sample, a middle electrode layer for sealing the microfluid channel to form an intact capillary and providing the needed voltage for electrophoresis; and a lower heating layer for providing a stable temperature gradient for electrophoresis. The upper, middle and lower layers are thermal conductive and adhesive to each other.

Abstract translation: 本发明提供一种用于检测属于毛细管电泳装置的核苷酸多态性或单核苷酸多态性的毛细管电泳芯片装置。 该装置包括上通道层，其包括一维，二维或多维微流体通道和用于装载样品的电极孔结构，用于密封微流体通道以形成完整毛细管的中间电极层，并提供所需电压 电泳; 以及用于提供电泳的稳定温度梯度的较低加热层。 上，中，下层相互导热，粘合。

公开(公告)号：US09677133B2

公开(公告)日：2017-06-13

申请号：US14006540

申请日：2012-02-10

Applicant: Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

Inventor： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

IPC: C12Q1/68 ,  G01N33/50 ,  G01N33/543 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: C12Q1/6876 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0622 ,  G01N33/543 ,  G01N2035/00158

Abstract: A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.

公开(公告)号：US09101924B2

公开(公告)日：2015-08-11

申请号：US14006512

申请日：2012-02-10

Applicant: Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

Inventor： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/49 ,  B01L3/00

CPC classification number: B01L3/502 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/0487

Abstract: An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.

Abstract translation: 用于生物芯片的接口装置至少包括接口单元，所述接口单元由仪器接口层，流体通道层和样品界面层组成。 仪器界面层至少有一个仪器界面。 流体通道层具有一个中空的流体通道。 样品接口层具有至少一个样品接口，并且所述流体通道的两端分别连接到样品接口和仪器接口。 界面设备通过气体或液体在流体通道中将样品溶液与仪器界面分离，从而避免样品溶液与仪器界面之间的直接接触，这将导致污染，并在使用仪器后省略清洁过程。 结构简单，操作方便，成本低，适用于化学领域，生物学领域和医学分析领域。

公开(公告)号：US08901913B2

公开(公告)日：2014-12-02

申请号：US12424955

申请日：2009-04-16

Applicant: Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

Inventor： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC: G01N27/00 ,  G01N33/483

CPC classification number: G01N33/4836

Abstract: Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

Abstract translation: 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US20140011707A1

公开(公告)日：2014-01-09

申请号：US14006540

申请日：2012-02-10

Applicant: Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

Inventor： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Liqing Duan ,  Kaijun Zhao ,  Jinxiu Zhang ,  Xiumei Song ,  Dong Wang ,  Huafang Gao ,  Wanli Xing

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0622 ,  G01N33/543 ,  G01N2035/00158

Abstract: A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.

Abstract translation: 提供了一种杂交系统，其包括具有带有探针点阵（3）的基底（1）和具有至少两个通孔（4）的盖板（2）的生物芯片，其中混合室（5） 形成在基板（1）和盖板（2）之间，至少两个流体通道（6）分别通过两个通孔（4）与混合室（5）相互连接，并且流体控制装置 与流体通道（6）互连。 生物芯片杂交系统集成了杂交，清洁和干燥功能，使用由流体控制装置提供的电力作为促进液体流动的驱动力，用于在流体通道（6）和腔室（5）中自动往复流动，以实现 动态杂交，从而提高混合效率和均匀性，实现自动控制。

公开(公告)号：US20140010733A1

公开(公告)日：2014-01-09

申请号：US14006512

申请日：2012-02-10

Applicant: Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

Inventor： Jiaming Ye ,  Pinhong Wang ,  Guoqing Wang ,  Wanli Xing

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/0487

Abstract: An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.

Abstract translation: 用于生物芯片的接口装置至少包括接口单元，所述接口单元由仪器接口层，流体通道层和样品界面层组成。 仪器界面层至少有一个仪器界面。 流体通道层具有一个中空的流体通道。 样品接口层具有至少一个样品接口，并且所述流体通道的两端分别连接到样品接口和仪器接口。 界面设备通过液体通道中的气体或液体将样品溶液与仪器界面分离，从而避免样品溶液与仪器界面之间的直接接触，从而造成污染，并在使用仪器后省略清洗过程。 结构简单，操作方便，成本低，适用于化学领域，生物学领域和医学分析领域。

公开(公告)号：US20130267436A1

公开(公告)日：2013-10-10

申请号：US13877320

申请日：2010-10-27

Applicant: Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

Inventor： Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

IPC: G01N33/58 ,  C12Q1/68

CPC classification number: G01N33/582 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C40B30/04 ,  G01N33/54333 ,  G01N33/54386 ,  C12Q2563/103 ,  C12Q2565/501

Abstract: A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

Abstract translation: 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括用发光体标记目标分子，将靶分子偶联到颗粒上，并在微阵列上结合探针分子。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 该技术具有高灵敏度，能够以有效的方式检测和解释分子相互作用。

公开(公告)号：US20130244270A1

公开(公告)日：2013-09-19

申请号：US13814426

申请日：2011-08-10

Applicant: Lan Xie ,  Rui Ma ,  Chao Han ,  Wanli Xing ,  Jing Cheng

Inventor： Lan Xie ,  Rui Ma ,  Chao Han ,  Wanli Xing ,  Jing Cheng

IPC: C12Q1/02

CPC classification number: C12Q1/025 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0864 ,  G01N33/5029

Abstract: The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels.

Abstract translation: 本发明涉及用于细胞运动筛选和趋化性测试的微流体装置，其包括微流体通道和腔室。 可以将化学引诱物或化学引诱物分泌的细胞选择性地种植在微流体装置中，并且可以在通道中建立稳定的化学引诱物或化学增益梯度。

公开(公告)号：US08293519B2

公开(公告)日：2012-10-23

申请号：US10566329

申请日：2003-08-13

Applicant: Feijun Xian ,  Wanli Xing ,  Jing Cheng ,  Dong Liang ,  Liang Zhang ,  Dong Wang

Inventor： Feijun Xian ,  Wanli Xing ,  Jing Cheng ,  Dong Liang ,  Liang Zhang ,  Dong Wang

IPC: C12M1/36 ,  G01N15/06 ,  B01L3/00

CPC classification number: B01L3/50853 ,  B01J2219/00281 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L9/52 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  C12Q1/6837 ,  C40B60/14 ,  G01N33/54366

Abstract: This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.

Abstract translation: 本发明一般涉及微阵列芯片领域及其用途。 特别地，本发明提供一种微阵列反应装置，其可用于测定各种部分之间的相互作用，例如核酸，涉及蛋白质的免疫反应，蛋白质与核酸之间的相互作用，配体 - 受体相互作用和小分子，以及 蛋白质或核酸相互作用等。还提供了包含微阵列反应装置的生产制品和试剂盒以及使用微阵列反应装置的测定方法。

公开(公告)号：US09511366B2

公开(公告)日：2016-12-06

申请号：US13991848

申请日：2012-01-13

Applicant: Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

Inventor： Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

IPC: B01L3/00 ,  C12M3/00 ,  C12M1/32 ,  C12M3/06

CPC classification number: B01L3/5027 ,  C12M21/06 ,  C12M23/12 ,  C12M23/16

Abstract: A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.

Abstract translation: 微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。 微结构层（5）包括一个底层和多个微结构，用于定位样品。 覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。 定位井（6）位于微结构的正上方并相互连接。 入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。 微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。