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公开(公告)号：US09677133B2
公开(公告)日：2017-06-13
申请号：US14006540
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
CPC分类号： C12Q1/6876 , B01L3/5027 , B01L7/52 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0622 , G01N33/543 , G01N2035/00158
摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.
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公开(公告)号：US20140011707A1
公开(公告)日：2014-01-09
申请号：US14006540
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , B01L3/5027 , B01L7/52 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0622 , G01N33/543 , G01N2035/00158
摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.
摘要翻译： 提供了一种杂交系统，其包括具有带有探针点阵（3）的基底（1）和具有至少两个通孔（4）的盖板（2）的生物芯片，其中混合室（5） 形成在基板（1）和盖板（2）之间，至少两个流体通道（6）分别通过两个通孔（4）与混合室（5）相互连接，并且流体控制装置 与流体通道（6）互连。 生物芯片杂交系统集成了杂交，清洁和干燥功能，使用由流体控制装置提供的电力作为促进液体流动的驱动力，用于在流体通道（6）和腔室（5）中自动往复流动，以实现 动态杂交，从而提高混合效率和均匀性，实现自动控制。
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公开(公告)号：US20090143245A1
公开(公告)日：2009-06-04
申请号：US12093504
申请日：2006-02-20
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
CPC分类号： C12Q1/6881 , C12Q1/6837 , C12Q2600/156
摘要： The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.
摘要翻译： 本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段 。 本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。 还提供了使用和制造微阵列的方法。
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公开(公告)号：US20090156419A1
公开(公告)日：2009-06-18
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C40B30/04
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译： 本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。 除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。 将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。
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公开(公告)号：US09200323B2
公开(公告)日：2015-12-01
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译： 本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。 除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。 将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。
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公开(公告)号：US07718362B2
公开(公告)日：2010-05-18
申请号：US10562803
申请日：2003-07-18
申请人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
CPC分类号： C12Q1/6837
摘要： This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译： 本发明一般涉及核酸分析领域。 特别地，本发明提供了一种用于对目标基因进行分型的方法，特别地，使用包含适合用于核酸杂交的载体的芯片，其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下 寡核苷酸对照探针：阳性对照探针，阴性对照探针，杂交对照探针和固定化对照探针。 还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。
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公开(公告)号：US20070134661A1
公开(公告)日：2007-06-14
申请号：US10562803
申请日：2003-07-18
申请人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
CPC分类号： C12Q1/6837
摘要： This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译： 本发明一般涉及核酸分析领域。 特别地，本发明提供了一种用于对目标基因进行分型的方法，特别地，使用包含适合用于核酸杂交的载体的芯片，其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下 寡核苷酸对照探针：阳性对照探针，阴性对照探针，杂交对照探针和固定化对照探针。 还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。
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公开(公告)号：US08293519B2
公开(公告)日：2012-10-23
申请号：US10566329
申请日：2003-08-13
申请人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
发明人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
CPC分类号： B01L3/50853 , B01J2219/00281 , B01J2219/00596 , B01J2219/00659 , B01L9/52 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , C40B60/14 , G01N33/54366
摘要： This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.
摘要翻译： 本发明一般涉及微阵列芯片领域及其用途。 特别地，本发明提供一种微阵列反应装置，其可用于测定各种部分之间的相互作用，例如核酸，涉及蛋白质的免疫反应，蛋白质与核酸之间的相互作用，配体 - 受体相互作用和小分子，以及 蛋白质或核酸相互作用等。还提供了包含微阵列反应装置的生产制品和试剂盒以及使用微阵列反应装置的测定方法。
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公开(公告)号：US20070054273A1
公开(公告)日：2007-03-08
申请号：US10566329
申请日：2003-08-13
申请人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
发明人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
CPC分类号： B01L3/50853 , B01J2219/00281 , B01J2219/00596 , B01J2219/00659 , B01L9/52 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , C40B60/14 , G01N33/54366
摘要： This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.
摘要翻译： 本发明一般涉及微阵列芯片领域及其用途。 特别地，本发明提供一种微阵列反应装置，其可用于测定各种部分之间的相互作用，例如核酸，涉及蛋白质的免疫反应，蛋白质与核酸之间的相互作用，配体 - 受体相互作用和小分子，以及 蛋白质或核酸相互作用等。还提供了包含微阵列反应装置的生产制品和试剂盒以及使用微阵列反应装置的测定方法。
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公开(公告)号：US11075442B2
公开(公告)日：2021-07-27
申请号：US15610085
申请日：2017-05-31
申请人： Dong Wang , Enliang Wang
发明人： Dong Wang , Enliang Wang
摘要： Antennas and MIMO antenna systems in a housing of an electronic device are described. Each of the antennas includes a first RF radiating member having a first frequency range and a second RF radiating member having a second frequency range. The first frequency range is 4-5 GHz and the second frequency range is 3-4 GHz, and each antenna has an operating frequency range of at least 3-5 GHz. A plurality of the antennas may be arranged in a housing of an electronic device to form MIMO antenna systems.
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