摘要:
The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.
摘要:
Method for registering filling potential of a waste container of a microplate washing device has a needle for aspirating liquids, a waste container, a pump for generating a partial vacuum and a pressure sensor for determining air pressure in the container, a sensor controller, and a first valve for blocking the line between the needle and the waste container. The pump and needle are connected to the container and the method includes closing the first valve, generating a partial vacuum in a test range, closing a further valve between the container and the pump, opening the first valve, triggering a partial vacuum dissipation in the container, measuring a test time for the partial vacuum dissipation in the test range, comparing the test time to a known threshold time, and deciding that the waste container is fillable or not.
摘要:
A device, appropriate process units and/or systems for providing a gap-shaped hybridization space to hybridize nucleic acid samples, proteins or tissue sections over a slide. Said items of device are able to move in relation to the slide and comprise an annular sealing surface to seal the hybridization space by application to a surface of the slide. Said items of device additionally comprise lines to introduce media to and remove them from the hybridization space. Said items of device furthermore comprise a specimen supply line and preferably an agitation device to move fluids in relation to the samples immobilized on the surface of the slide. The devices according to the invention are characterized in that they limit a hybridization space that features relief structures to conduct and/or block air bubbles on at least one surface of the device and the slide directed at the interior of this hybridization space. According to the method of the invention, air bubbles present in the hybridization space are conducted and/or blocked by these relief structures.
摘要:
A device (1), appropriate process units (18) and/or systems (38) for providing a gap-shaped hybridization space (2) to hybridize nucleic acid samples, proteins or tissue sections over a slide (3). Said items of device (1) are able to move in relation to the slide (3) and comprise an annular sealing surface (4) to seal the hybridization space (2) by application to a surface (5) of the slide (3). Said items of device (1) additionally comprise lines (6,6′) to introduce media to and remove them from the hybridization space (2). Said items of device (1) furthermore comprise a specimen supply line (7) and preferably an agitation device (8) to move fluids in relation to the samples immobilized on the surface (5) of the slide (3). The devices according to the invention are characterized in that they limit a hybridization space (2) that features relief structures (101) to conduct and/or block air bubbles (102) on at least one surface (5,14) of the device (1) and the slide (3) directed at the interior of this hybridization space (2). According to the method of the invention, air bubbles (102) present in the hybridization space (2) are conducted and/or blocked by these relief structures (101).
摘要:
A device (1) is disclosed for providing a hybridization chamber (2), for hybridizing nucleic acid samples, proteins, or tissue sections on a slide (3), which is implemented so that it is movable in relation to this slide (3) and includes an annular sealing surface (4) for sealing the hybridization chamber (2) by being applied to a surface (5) of this slide (3), lines (6) for supplying and/or removing media to and/or from the hybridization chamber (2), and a specimen supply line (7). The devices according to the invention include a media-separating agitation device (8) for moving liquids in relation to samples immobilized on the surface (5) of the slide (3). Furthermore, process units (18) for hybridizing nucleic acid samples, proteins, or tissue sections and corresponding automatable systems including such process units (18) are disclosed.