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    Site-specific labeling of affinity tags in fusion proteins
    11.
    发明申请
    Site-specific labeling of affinity tags in fusion proteins 审中-公开
    融合蛋白中亲和标签的位点特异性标记

    公开(公告)号：US20050233307A1

    公开(公告)日：2005-10-20

    申请号：US10661451

    申请日：2003-09-12

    Applicant: Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    Inventor： Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    IPC: A61K49/00 ,  C07D311/12 ,  C07D311/18 ,  C07F5/02 ,  C12Q1/00 ,  G01N20060101 ,  G01N33/00 ,  G01N33/52 ,  G01N33/533

    CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

    Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

    Method for measuring cellular transmembrane potential changes
    12.
    发明授权
    Method for measuring cellular transmembrane potential changes 有权
    测量细胞跨膜电位变化的方法

    公开(公告)号：US06852504B2

    公开(公告)日：2005-02-08

    申请号：US09924797

    申请日：2001-08-08

    Applicant: Dieter Klaubert ,  Zhenjun Diwu ,  Guoliang Yi ,  Martin Kirk

    Inventor： Dieter Klaubert ,  Zhenjun Diwu ,  Guoliang Yi ,  Martin Kirk

    IPC: G01N33/52 ,  C12Q1/02

    CPC classification number: G01N33/52 ,  Y10S435/975

    Abstract: The invention encompasses an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Device Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells

    Abstract translation: 本发明包括使用式I化合物作为电位探针来测量膜电位的改进方法。 这些探针可以与其它荧光指示剂如Indo-1，Fura-2和Fluo-3组合使用，这样的探针可以用于微孔板读取装置，例如FLIPR TM，荧光成像板读数器，由Molecular Device Corp.，Sunnyvale，Calif。 流式细胞仪 和荧光计。 这种探针用于测量活细胞中的膜电位

    Rhodamine lactone phosphoramidites and polymers
    16.
    发明申请
    Rhodamine lactone phosphoramidites and polymers 审中-公开
    罗丹明内酯亚磷酰胺和聚合物

    公开(公告)号：US20120016128A1

    公开(公告)日：2012-01-19

    申请号：US13135178

    申请日：2011-06-27

    Applicant: Zhenjun Diwu ,  Haitao Guo

    Inventor： Zhenjun Diwu ,  Haitao Guo

    IPC: C07F7/02 ,  C07F9/6596 ,  C07F9/6561

    CPC classification number: C07F9/6561 ,  C07F9/65517 ,  C07F9/6596

    Abstract: The present invention provides rhodamine lactone phosphoramidites and polymer compositions, methods for making these phosphoramidites and polymer compositions, and methods for using these phosphoramidites and polymer compositions for labeling oligonucleotides. In particular, the present invention provides compositions and methods for labeling the 3′- and 5′-end of oligonucleotides during synthesis of the oligonucleotides.

    Abstract translation: 本发明提供罗丹明内酯亚磷酰胺和聚合物组合物，制备这些亚磷酰胺和聚合物组合物的方法，以及使用这些亚磷酰胺和用于标记寡核苷酸的聚合物组合物的方法。 特别地，本发明提供了在寡核苷酸合成期间标记寡核苷酸的3'-末端和5'末端的组合物和方法。

    QUINAZOLINE-CONTAINING KITS FOR LABELING ALDEHYDE OR KETONE MOIETIES
    18.
    发明申请
    QUINAZOLINE-CONTAINING KITS FOR LABELING ALDEHYDE OR KETONE MOIETIES 审中-公开
    用于标示醛或酮类的含喹诺酮的包装盒

    公开(公告)号：US20110117659A1

    公开(公告)日：2011-05-19

    申请号：US12906014

    申请日：2010-10-15

    Applicant: Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    Inventor： Richard P. Haugland ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Zhenjun Diwu

    IPC: G01N33/68 ,  G01N33/00

    CPC classification number: C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

    Abstract: Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

    Abstract translation: 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R5是-L-Z; L是亚芳基或C1-6全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-6烷基，C1-6烷氧基，C1-6全氟烷基，C1-6烷基氨基，二（C2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基， 或磺基; 和R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基， 苯基，磺基或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

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