公开(公告)号：US20130084609A1

公开(公告)日：2013-04-04

申请号：US13707950

申请日：2012-12-07

Applicant: AJINOMOTO CO., INC.

Inventor： Shigeo Suzuki ,  Yoshihiro Usuda ,  Shuhei Hashiro

IPC: C12P13/04

CPC classification number: C12P13/04 ,  C12P13/08 ,  C12P13/14

Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.

Abstract translation: 通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。 通过破坏微藻的培养和/或提取微藻的培养物，或分馏微藻的培养物或破坏的培养物来生产该过程产物。 经加工的产品含有由微藻生产的有机物质，破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。 经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。 培养细菌以在培养物中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。

公开(公告)号：US20180044703A1

公开(公告)日：2018-02-15

申请号：US15787861

申请日：2017-10-19

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14 ,  C12P7/50 ,  C12P7/48 ,  C12P7/46 ,  C07K14/265 ,  C07K14/245 ,  C12P13/20 ,  C07K14/195

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US08951760B2

公开(公告)日：2015-02-10

申请号：US13912313

申请日：2013-06-07

Applicant: Ajinomoto Co., Inc.

Inventor： Hidetaka Doi ,  Yasushi Hoshino ,  Yuri Masumitsu ,  Yoshihiro Usuda

IPC: C12P13/08 ,  C12P13/04 ,  C07K14/245 ,  C12N1/20 ,  C12N1/38 ,  C12N15/74

CPC classification number: C12P13/08 ,  C07K14/245 ,  C12N1/20 ,  C12N1/38 ,  C12N15/746 ,  C12P13/04

Abstract: A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium. A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.

Abstract translation: L-氨基酸的制造方法包括在含有选自脂肪酸和醇的碳源的培养基中培养属于肠杆菌科的细菌并具有L-氨基酸生产能力的细菌， 来自培养基的氨基酸。 使用已经进行了包括oxyS基因表达增强，固定化ABC基因表达的增强及其组合中的至少一种的修饰的细菌作为细菌，或者添加减少细菌的细胞内过氧化氢浓度的物质 到中等

公开(公告)号：US08728772B2

公开(公告)日：2014-05-20

申请号：US13707950

申请日：2012-12-07

Applicant: Ajinomoto Co., Inc.

Inventor： Shigeo Suzuki ,  Yoshihiro Usuda ,  Shuhei Hashiro

IPC: C12P13/04 ,  C12P13/14 ,  C12P13/16

CPC classification number: C12P13/04 ,  C12P13/08 ,  C12P13/14

Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.

Abstract translation: 通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。 通过破坏微藻的培养和/或提取微藻的培养物，或分馏微藻的培养物或破坏的培养物来生产该过程产物。 经加工的产品含有由微藻生产的有机物质，破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。 经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。 培养细菌以在培养物中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。

公开(公告)号：US20130288313A1

公开(公告)日：2013-10-31

申请号：US13938601

申请日：2013-07-10

Applicant: Ajinomoto Co., Inc.

Inventor： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12P13/04

Abstract: An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

Abstract translation: 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。