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29 条记录 (耗时 0.041 秒)

    Multiply-substituted protease variants with altered net charge for use in detergents
    11.
    发明申请
    Multiply-substituted protease variants with altered net charge for use in detergents 审中-公开

    公开(公告)号：US20080274938A1

    公开(公告)日：2008-11-06

    申请号：US11588435

    申请日：2006-10-27

    申请人： Ayrookaran J. Poulose ,  Volker Schellenberger ,  James T. Kellis ,  Paech Christian ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    发明人： Ayrookaran J. Poulose ,  Volker Schellenberger ,  James T. Kellis ,  Paech Christian ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    IPC分类号： C11D7/42 ,  C12N9/56

    CPC分类号： A23G4/123 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C11D3/386 ,  C12N9/54 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease.Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease.Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

    Mutant &agr;-amylase
    14.
    发明授权
    Mutant &agr;-amylase 失效
    突变型α-淀粉酶

    公开(公告)号：US06211134B1

    公开(公告)日：2001-04-03

    申请号：US08985659

    申请日：1997-12-09

    申请人： Robert M. Caldwell ,  Colin Mitchinson ,  Traci H Ropp

    发明人： Robert M. Caldwell ,  Colin Mitchinson ,  Traci H Ropp

    IPC分类号： C11D3386

    CPC分类号： C12N9/2417

    摘要： Novel &agr;-amylase enzymes are disclosed having a substution equivalent to G475R in Bacillus licheniformis. The disclosed &agr;-amylase enzymes show improved specific activity and starch hydrolysis performance. Also provided are polynucleotides encoding such enzymes, expression vectors including such polynucleotides, host cells transformed with such expression vectors, and the use of such enzymes in detergent compositions.

    摘要翻译： 公开了具有等同于地衣芽孢杆菌中的G475R的分子的新型α-淀粉酶。 所公开的α-淀粉酶显示改善的比活性和淀粉水解性能。 还提供了编码这些酶的多核苷酸，包括这种多核苷酸的表达载体，用这种表达载体转化的宿主细胞，以及这些酶在洗涤剂组合物中的用途。

    Subtilisin mutants
    15.
    发明授权
    Subtilisin mutants 失效

    公开(公告)号：US5204015A

    公开(公告)日：1993-04-20

    申请号：US958885

    申请日：1992-10-09

    申请人： Robert M. Caldwell ,  David A. Estell ,  Thomas P. Graycar

    发明人： Robert M. Caldwell ,  David A. Estell ,  Thomas P. Graycar

    IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N9/56 ,  C12N15/10 ,  C12N15/75

    CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

    摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.

    Increasing production of proteins in gram-positive microorganisms using SecG
    18.
    发明授权
    Increasing production of proteins in gram-positive microorganisms using SecG 有权
    使用SecG增加革兰氏阳性微生物中蛋白质的产量

    公开(公告)号：US06506579B1

    公开(公告)日：2003-01-14

    申请号：US09462843

    申请日：2000-03-22

    申请人： Wilhelmus J. Quax ,  Robert M. Caldwell

    发明人： Wilhelmus J. Quax ,  Robert M. Caldwell

    IPC分类号： C12P1206

    CPC分类号： C07K14/32 ,  C07K2319/00 ,  C12N15/75 ,  C12P21/02

    摘要： The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in gram-positive microorganisms.

    摘要翻译： 本发明涉及革兰氏阳性微生物中的分泌。 本发明提供枯草芽孢杆菌分泌因子SecG的核酸和氨基酸序列。 本发明还提供增加革兰氏阳性微生物中异源或同源蛋白质分泌的方法。