公开(公告)号：US20240229137A9

公开(公告)日：2024-07-11

申请号：US18382797

申请日：2023-10-23

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  G01N33/5308 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US20240132961A1

公开(公告)日：2024-04-25

申请号：US18382797

申请日：2023-10-22

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  G01N33/5308 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US20230348975A1

公开(公告)日：2023-11-02

申请号：US18187488

申请日：2023-03-21

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Q2563/185

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US20230340566A1

公开(公告)日：2023-10-26

申请号：US18186940

申请日：2023-03-20

Applicant: Becton, Dickinson and Company

Inventor： Christina Chang ,  Eleen Shum

IPC: C12Q1/6806 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  C12Q1/686

Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. There are provided, in some embodiments, methods of 5′-based and 3′-based gene expression profiling. Immune repertoire profiling methods are also provided in some embodiments.

公开(公告)号：US11965208B2

公开(公告)日：2024-04-23

申请号：US16849191

申请日：2020-04-15

Applicant: Becton, Dickinson and Company

Inventor： Christina Chang ,  Sixing Li

IPC: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858

CPC classification number: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.

公开(公告)号：US11460468B2

公开(公告)日：2022-10-04

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B25/10 ,  G16H20/13 ,  G16H10/20

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US11371076B2

公开(公告)日：2022-06-28

申请号：US16743898

申请日：2020-01-15

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk ,  Eleen Shum ,  Christina Chang

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.

公开(公告)号：US20200224247A1

公开(公告)日：2020-07-16

申请号：US16743898

申请日：2020-01-15

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk ,  Eleen Shum ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.