公开(公告)号：US09580732B2

公开(公告)日：2017-02-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 一种方法，包括步骤a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1是-OH，-SH，-NH 2或-COOR 2; x至少为3; 并且R2是H，烷基或芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a）的氧化产物与转氨酶接触，其中NAD ）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶。 用于实施该方法的全细胞催化剂。 使用这种全细胞催化剂来氧化伯醇。

公开(公告)号：US09005928B2

公开(公告)日：2015-04-14

申请号：US13812625

申请日：2011-07-20

申请人： Steffen Schaffer ,  Mirja Wessel ,  Anja Thiessenhusen ,  Nadine Stein

发明人： Steffen Schaffer ,  Mirja Wessel ,  Anja Thiessenhusen ,  Nadine Stein

IPC分类号： C12N1/20 ,  C12P19/44 ,  A01N43/16 ,  C12N9/10

CPC分类号： C12N15/78 ,  A01N43/16 ,  C12N9/1051 ,  C12N9/14 ,  C12N15/52 ,  C12N15/70 ,  C12N15/77 ,  C12P19/44 ,  C12Y306/04013 ,  Y02P20/52

摘要： The invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids.

摘要翻译： 本发明涉及细胞和核酸，以及其用于制备鼠李糖脂的用途，以及用于制备鼠李糖脂的方法。

公开(公告)号：US08986961B2

公开(公告)日：2015-03-24

申请号：US12679488

申请日：2008-10-01

申请人： Stefan Verseck ,  Steffen Schaffer ,  Werner Freitag ,  Friedrich-Georg Schmidt ,  Matthias Orschel ,  Gerda Grund ,  Wilfried Schmidt ,  Hubert Johannes Bahl ,  Ralf-Joerg Fischer ,  Antje May ,  Peter Duerre ,  Simone Lederle

发明人： Stefan Verseck ,  Steffen Schaffer ,  Werner Freitag ,  Friedrich-Georg Schmidt ,  Matthias Orschel ,  Gerda Grund ,  Wilfried Schmidt ,  Hubert Johannes Bahl ,  Ralf-Joerg Fischer ,  Antje May ,  Peter Duerre ,  Simone Lederle

IPC分类号： C12P7/26 ,  C12P7/28 ,  C12N9/16 ,  C12N9/00

CPC分类号： C12P7/28 ,  C12N9/16 ,  C12N9/93 ,  C12Y301/02 ,  C12Y301/02018

摘要： The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO2, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalyzed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

摘要翻译： 本发明描述了从乙酰辅酶A开始制备丙酮的方法，包括方法步骤A.将乙酰辅酶A酶促转化为乙酰乙酰辅酶A.将乙酰乙酰辅酶A酶促转化为乙酰乙酸酯，并将乙酰乙酸酯脱羧至丙酮， CO 2，其特征在于辅酶A在工艺步骤B中不转移到受体分子。 此外，方法步骤B令人惊奇地由酰基-CoA硫酯酶，酰基辅酶A合成酶或酰基辅酶A硫代激酶类的酶催化。 关注一个完全新颖的代谢途径，因为乙酰乙酰辅酶A的酶水解而不会同时转移CoA到受体分子，从未以前没有描述任何微生物酶。

公开(公告)号：US20140308717A1

公开(公告)日：2014-10-16

申请号：US14237121

申请日：2012-07-27

申请人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

发明人： Thomas Haas ,  Markus Poetter ,  Jan Christoph Pfeffer ,  Wolfgang Kroutil ,  Arne Skerra ,  Alexandra Lerchner ,  Katharina Christin Tauber ,  Johann H. Sattler ,  Steffen Schaffer

IPC分类号： C12P13/00 ,  C12P17/04 ,  C12N15/63

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/1096 ,  C12N15/63 ,  C12P13/005 ,  C12P13/04 ,  C12P17/04 ,  C12Y101/01001 ,  C12Y206/01 ,  C12Y206/01018

摘要： The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供仲醇，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化仲醇，和c）将步骤a）的氧化产物与 转氨酶，其中NAD（P）+依赖性醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂来氧化二级 醇。

公开(公告)号：US20140242646A1

公开(公告)日：2014-08-28

申请号：US14233505

申请日：2012-07-17

申请人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

发明人： Markus Pötter ,  Thomas Haas ,  Jan Christoph Pfeffer ,  Arne Skerra ,  Wolfgang Kroutil ,  Alexandra Lerchner ,  Johann H. Sattler ,  Steffen Schaffer ,  Katharina Christin Tauber

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  Y02P20/582

摘要： The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1选自-OH，-SH，-NH 2和-COOR 2，x 至少3，R2选自H，烷基和芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a的氧化产物 ）与转氨酶，其中NAD（P）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂进行氧化 一个小酒精。

公开(公告)号：US20130035403A1

公开(公告)日：2013-02-07

申请号：US13509716

申请日：2010-10-19

申请人： Steffen Schaffer ,  Mirja Wessel ,  Anja Thiessenhusen

发明人： Steffen Schaffer ,  Mirja Wessel ,  Anja Thiessenhusen

IPC分类号： C12P19/44 ,  C12N1/19 ,  C12N1/15 ,  C12N15/52 ,  A61K8/92 ,  C12N9/10 ,  C12N9/02 ,  C12N9/14 ,  A61K47/26 ,  C12N5/10 ,  C12N15/63

CPC分类号： C12P19/44 ,  A61K31/704 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1051 ,  C12P19/14

摘要： The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.

公开(公告)号：US09404118B2

公开(公告)日：2016-08-02

申请号：US14238248

申请日：2012-07-23

申请人： Tim Köhler ,  Christoph Schorsch ,  Eckhard Boles ,  Heiko Andrea ,  Mike Farwick ,  Steffen Schaffer

发明人： Tim Köhler ,  Christoph Schorsch ,  Eckhard Boles ,  Heiko Andrea ,  Mike Farwick ,  Steffen Schaffer

IPC分类号： C12N1/14 ,  C12N15/81 ,  C07K14/39 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P13/02

CPC分类号： C12P13/02 ,  C07K14/39 ,  C12N9/0006 ,  C12N9/0073 ,  C12N9/1014 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/815 ,  C12P7/6436 ,  C12Y114/13169 ,  C12Y203/0105 ,  C12Y207/01091 ,  C12Y401/02027 ,  Y02P20/52

摘要： The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.

摘要翻译： 本发明涉及遗传修饰的毕赤酵母细胞，其用途和生产类鞘氨醇碱和鞘脂的方法。

公开(公告)号：US20160060663A1

公开(公告)日：2016-03-03

申请号：US14843525

申请日：2015-09-02

申请人： Katrin GRAMMANN ,  Jan Wolter ,  Liv Reinecke ,  Steffen Schaffer ,  Eileen C. Spindler ,  Wendy K. Ribble ,  Brittany L. Prather ,  Catherine B. Poor ,  Tanya Warnecke Lipscomb ,  Hans H. Liao ,  Dave A. Hogsett ,  Ronald J. Evans

发明人： Katrin GRAMMANN ,  Jan Wolter ,  Liv Reinecke ,  Steffen Schaffer ,  Eileen C. Spindler ,  Wendy K. Ribble ,  Brittany L. Prather ,  Catherine B. Poor ,  Tanya Warnecke Lipscomb ,  Hans H. Liao ,  Dave A. Hogsett ,  Ronald J. Evans

IPC分类号： C12P7/64 ,  C12N9/10

CPC分类号： C12P7/6436 ,  C12N9/1029 ,  C12Y203/01041 ,  C12Y203/01075

摘要： A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

摘要翻译： 微生物细胞用于生产至少一种脂肪酸酯，其中所述细胞被遗传修饰以包含（i）至少一种能使细胞产生至少一种脂肪酸和/或酰基辅酶A（CoA）的第一遗传突变 ），其相对于丙二酰辅酶A依赖性和丙二酰-ACP无关脂肪酰基辅酶A代谢途径的野生型细胞增加细胞中的酶活性，其中所述脂肪酸含有至少5个碳原子; 和（ii）增加细胞中至少一种蜡酯合酶相对于野生型细胞的活性的第二种遗传突变，并且蜡酯合成酶与SEQ ID NO：1的多肽具有至少50％的序列同一性 -8及其组合或任何多肽的功能片段，用于催化脂肪酸和/或其酰基辅酶A转化为脂肪酸酯。

公开(公告)号：US20150218600A1

公开(公告)日：2015-08-06

申请号：US14363178

申请日：2012-11-14

申请人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

发明人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

IPC分类号： C12P7/42 ,  C12N9/88 ,  C12N9/12 ,  C12N9/02 ,  C12N9/00 ,  C12N9/10

CPC分类号： C12P7/42 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y103/99002 ,  C12Y207/02007 ,  C12Y402/01017 ,  C12Y602/01002

摘要： The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid.

摘要翻译： 本发明涉及包括以下步骤的方法：a）提供异丁酸，b）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶c的组合接触 ）使来自步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）将来自步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）中的产物以形成3-羟基异丁酸 酸，其中至少一种酶以细胞形式使用，与其野生型相比，其包含3-羟基异丁酸脱氢酶或其变体的降低的活性，具有至少一种酶的细胞 异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A水合酶和 3-羟基异丁酰辅酶A水解酶，与其野生型相比，3-羟基异丁酸脱氢酶或其变体的活性降低，其中细胞优选还具有单加氧酶，更优选alkBGT型的单加氧酶或 其变体以及这种细胞用于制备3-羟基异丁酸的用途。

公开(公告)号：US08999684B2

公开(公告)日：2015-04-07

申请号：US13764996

申请日：2013-02-12

申请人： Markus Poetter ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Thomas Haas

发明人： Markus Poetter ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Thomas Haas

IPC分类号： C12P7/40 ,  C12P13/04 ,  C12N1/16 ,  C12N9/02 ,  C12P7/42 ,  C12P7/62

CPC分类号： C12P7/62 ,  C07C51/347 ,  C07C67/42 ,  C07C227/06 ,  C07C227/08 ,  C12N1/16 ,  C12N9/004 ,  C12N9/0042 ,  C12P7/42 ,  C12P7/625 ,  C12P13/04 ,  C12Y101/01001

摘要： The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

摘要翻译： 本发明涉及基因工程的热带假丝酵母细胞，其用途和生产ω-羟基羧酸和ω-羟基羧酸酯的方法。