公开(公告)号：US10017804B2

公开(公告)日：2018-07-10

申请号：US14764898

申请日：2014-02-11

Applicant: Evolva SA

Inventor： Ernesto Simon ,  Iben Nordmark Anderson ,  Michael Dalgaard Mikkelsen ,  Jorgen Hansen ,  Veronique Douchin

IPC: C12P19/56 ,  C12N9/02 ,  C07K14/395 ,  C12N9/10 ,  C12P15/00 ,  C12N9/88 ,  C12N9/90

CPC classification number: C12P19/56 ,  C07K14/395 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1062 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12P15/00 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y204/00 ,  C12Y204/01013 ,  C12Y204/01035 ,  C12Y205/01029 ,  C12Y402/03 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretion of steviol glycosides of interest.

公开(公告)号：US20250027131A1

公开(公告)日：2025-01-23

申请号：US18756786

申请日：2024-06-27

Applicant: EVOLVA SA

Inventor： Ganesh M. Kishore ,  Michael Motion ,  Paula M. Hicks ,  Jorgen Hansen ,  Jens Houghton Larsen ,  Esben Halkjaer Hansen ,  Michael Dalgaard Mikkelsen ,  Sabina Tavares ,  Charlotte Blom

IPC: C12P19/56 ,  A23L2/60 ,  A23L27/30 ,  C07H15/256 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/82 ,  C12P7/42 ,  C12P15/00

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

公开(公告)号：US20240110215A1

公开(公告)日：2024-04-04

申请号：US18470785

申请日：2023-09-20

Applicant: EVOLVA SA

Inventor： Ganesh M. Kishore ,  Michael Motion ,  Paula M. Hicks ,  Jorgen Hansen ,  Jens Houghton Larsen ,  Esben Halkjaer Hansen ,  Michael Dalgaard Mikkelsen ,  Sabina Tavares ,  Charlotte Blom

IPC: C12P19/56 ,  A23L2/60 ,  A23L27/30 ,  C07H15/256 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/82 ,  C12P7/42 ,  C12P15/00

CPC classification number: C12P19/56 ,  A23L2/60 ,  A23L27/36 ,  C07H15/256 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/1077 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/8243 ,  C12N15/8245 ,  C12P7/42 ,  C12P15/00 ,  C12Y101/01088 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y114/13079 ,  C12Y204/01126 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012 ,  Y02P20/52

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

公开(公告)号：US20220073960A1

公开(公告)日：2022-03-10

申请号：US17373612

申请日：2021-07-12

Applicant: EVOLVA SA

Inventor： Ganesh M. Kishore ,  Michael Motion ,  Paula M. Hicks ,  Jorgen Hansen ,  Jens Hougton-Larsen ,  Esben Halkjaer Hansen ,  Michael Dalgaard Mikkelsen ,  Sabina Tavares ,  Charlotte Blom, Sr.

IPC: C12P19/56 ,  A23L27/30 ,  C12N15/82 ,  C12P15/00 ,  C12N9/10 ,  A23L2/60 ,  C07H15/256 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P7/42

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

公开(公告)号：US20220017932A1

公开(公告)日：2022-01-20

申请号：US17244089

申请日：2021-04-29

Applicant: EVOLVA SA

Inventor： Ernesto Simon ,  Iben Nordmark Anderson ,  Michael Dalgaard Mikkelsen ,  Jorgen Hansen ,  Veronique Douchin

IPC: C12P19/56 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C07K14/395 ,  C12N9/10 ,  C12P15/00

Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretion of steviol glycosides of interest.

公开(公告)号：US10662458B2

公开(公告)日：2020-05-26

申请号：US15900557

申请日：2018-02-20

Applicant: EVOLVA SA

Inventor： Yaoquan Liu ,  Jorgen Hansen ,  Jens Houghton-Larsen ,  Muthuswamy Panchapagesa Murali ,  Sathish Kumar ,  Nina Nicoline Rasmussen

IPC: C12P19/18 ,  C12P33/20 ,  C12N9/10 ,  C12P19/56 ,  C12P33/08 ,  C12N9/02 ,  C12N9/14 ,  C12N9/90 ,  C12P33/00 ,  C12P33/12 ,  C12N15/80

Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

公开(公告)号：US10435730B2

公开(公告)日：2019-10-08

申请号：US15439660

申请日：2017-02-22

Applicant: Evolva SA

Inventor： Jens Houghton-Larsen ,  Paula Hicks ,  Michael Naesby ,  Thomas Tange Ostergaard ,  Jorgen Hansen ,  Michael Mikkelsen Dalgaard ,  Esben Hansen Halkjaer ,  Ernesto Simon ,  Sabina De Andrade Periera Tavares

IPC: C12P19/56 ,  C12N9/10 ,  C12N9/02 ,  C12N1/21 ,  C12N9/88 ,  C12N9/06 ,  C12N9/90 ,  C12N15/81 ,  C12N15/82 ,  C12P19/44 ,  C12N15/63

Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

公开(公告)号：US20190203243A1

公开(公告)日：2019-07-04

申请号：US15908214

申请日：2018-02-28

Applicant: EVOLVA SA

Inventor： Michael Dalgaard Mikkelsen ,  Jorgen Hansen ,  Ernesto Simon ,  Federico Brianza ,  Angelika Semmler ,  Kim Olsson ,  Simon Carlsen ,  Louis Düring ,  Alexei Ouspenski ,  Paula Hicks

IPC: C12P19/56 ,  C12N15/81 ,  C12N9/02 ,  C12N9/10 ,  A23L27/30 ,  A23L2/60 ,  C12N9/90

CPC classification number: C12P19/56 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/1081 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/81 ,  C12Y204/01 ,  C12Y204/99

Abstract: Methods for recombinant production of steviol glycoside and compositions containing steviol glycosides are provided by this invention.

公开(公告)号：US20180371517A1

公开(公告)日：2018-12-27

申请号：US16002245

申请日：2018-06-07

Applicant: EVOLVA SA

Inventor： Ernesto Simon ,  Iben Nordmark Anderson ,  Michael Dalgaard Mikkelsen ,  Jorgen Hansen ,  Veronique Douchin

IPC: C12P19/56 ,  C07K14/395 ,  C12N9/10 ,  C12N9/90 ,  C12N9/88 ,  C12N9/02 ,  C12P15/00

Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes or that overexpress one or more heterologous transporters, leading to increased excretions of steviol glucosides of interest.

公开(公告)号：US20180251806A1

公开(公告)日：2018-09-06

申请号：US15900557

申请日：2018-02-20

Applicant: EVOLVA SA

Inventor： Yaoquan Liu ,  Jorgen Hansen ,  Jens Houghton-Larsen ,  Muthuswamy Panchapagesa Murali ,  Sathish Kumar ,  Nina Nicoline Rasmussen

IPC: C12P33/20 ,  C12N9/02 ,  C12P33/12 ,  C12P33/08 ,  C12P33/00 ,  C12P19/56 ,  C12P19/18 ,  C12N9/90 ,  C12N9/14 ,  C12N9/10 ,  C12N15/80

Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.