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    Multiphase nucleic acid amplification
    11.
    发明授权
    Multiphase nucleic acid amplification 有权
    多相核酸扩增

    公开(公告)号：US09139870B2

    公开(公告)日：2015-09-22

    申请号：US14014607

    申请日：2013-08-30

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

    IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/70

    CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

    Abstract translation: 公开了用于核酸扩增的改进方法，包括多重扩增，其中扩增在两个或多个不同阶段中进行。 第一相扩增反应优选缺少指数扩增所需的一种或多种组分。 随后在第二，第三或另外的扩增相中提供缺乏的组分，导致快速指数扩增反应。 多相协议在低分析物浓度下导致更快更灵敏的检测和更低的变异性。 还公开了用于实施所要求保护的方法的组合物。

    BLOOD CELL LYSIS REAGENT
    16.
    发明申请
    BLOOD CELL LYSIS REAGENT 有权

    公开(公告)号：US20220002707A1

    公开(公告)日：2022-01-06

    申请号：US17485599

    申请日：2021-09-27

    Applicant: Gen-Probe Incorporated

    Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

    Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

    BLOOD CELL LYSIS REAGENT
    18.
    发明申请
    BLOOD CELL LYSIS REAGENT 审中-公开

    公开(公告)号：US20190136225A1

    公开(公告)日：2019-05-09

    申请号：US16094070

    申请日：2017-04-26

    Applicant: Gen-Probe Incorporated

    Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

    Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

    Red blood cell lysis solution
    20.
    发明授权
    Red blood cell lysis solution 有权

    公开(公告)号：US10093989B2

    公开(公告)日：2018-10-09

    申请号：US14918131

    申请日：2015-10-20

    Applicant: Gen-Probe Incorporated

    Inventor： Jijumon Chelliserry ,  Kui Gao ,  Jeffrey M. Linnen

    IPC: C12Q1/68 ,  C12Q1/6893 ,  C12N1/06 ,  G01N33/48

    Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

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