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公开(公告)号：US20210139886A1
公开(公告)日：2021-05-13
申请号：US17134604
申请日：2020-12-28
发明人： Kui Gao , Jijumon Chelliserry , Jeffrey Linnen
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/70
摘要： Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.
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公开(公告)号：US20130203123A1
公开(公告)日：2013-08-08
申请号：US13720108
申请日：2012-12-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C07H21/00 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C12Q2521/501 , C12Q2525/161 , C12Q2525/186 , C12Q2525/301 , C12Q2537/1373
摘要： The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
摘要翻译： 本发明提供用于制备其中一条或两条链是连续的封闭核酸结构的组合物和方法。 封闭的核酸结构可以用作其他应用中的测序模板。
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公开(公告)号：US12071615B2
公开(公告)日：2024-08-27
申请号：US17485599
申请日：2021-09-27
发明人： Kui Gao , Jijumon Chelliserry , Jeffrey Linnen
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/70
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q1/70 , Y02A50/30 , C12Q1/6806 , C12Q2525/161 , C12Q2527/125 , C12Q2531/143 , C12Q2565/501 , C12Q1/70 , C12Q2525/161 , C12Q2527/125 , C12Q2531/143 , C12Q2565/501
摘要： Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.
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公开(公告)号：US11859238B2
公开(公告)日：2024-01-02
申请号：US16241778
申请日：2019-01-07
IPC分类号： C12Q1/68 , C12Q1/6806 , C12Q1/6848 , C12Q1/70 , C12P19/34 , C12Q1/6865 , C12Q1/6825
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/68 , C12Q1/6825 , C12Q1/6848 , C12Q1/6865 , C12Q1/70 , C12Q1/6865 , C12Q2537/101 , C12Q2545/10
摘要： Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
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公开(公告)号：US11015185B1
公开(公告)日：2021-05-25
申请号：US17134604
申请日：2020-12-28
发明人： Kui Gao , Jijumon Chelliserry , Jeffrey Linnen
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/70
摘要： Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.
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公开(公告)号：US10689713B2
公开(公告)日：2020-06-23
申请号：US16019829
申请日：2018-06-27
发明人： Jijumon Chelliserry , Kui Gao , Jeffrey M. Linnen
IPC分类号： C12Q1/68 , C12Q1/6893 , C12N1/06 , G01N33/48 , C12Q1/6806
摘要： The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.
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公开(公告)号：US11162091B2
公开(公告)日：2021-11-02
申请号：US16094070
申请日：2017-04-26
发明人： Kui Gao , Jijumon Chelliserry , Jeffrey Linnen
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/70
摘要： Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.
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8.再颁专利公开(公告)号：USRE48732E1
公开(公告)日：2021-09-14
申请号：US16040383
申请日：2018-07-19
摘要： Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.
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公开(公告)号：US20180334727A1
公开(公告)日：2018-11-22
申请号：US16019829
申请日：2018-06-27
发明人： Jijumon Chelliserry , Kui Gao , Jeffrey M. Linnen
IPC分类号： C12Q1/6893 , C12N1/06 , G01N33/48
摘要： The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.
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10.发明申请公开(公告)号：US20170218435A1
公开(公告)日：2017-08-03
申请号：US15362692
申请日：2016-11-28
CPC分类号： C40B30/00 , C07H21/00 , C12N15/1065 , C12Q1/6811 , G16B25/00 , G16B30/00
摘要： Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.
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