公开(公告)号：US11447804B2

公开(公告)日：2022-09-20

申请号：US16178432

申请日：2018-11-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  A23L29/00 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US20190300919A1

公开(公告)日：2019-10-03

申请号：US16178432

申请日：2018-11-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/00 ,  A23L29/00 ,  C12N15/52 ,  C08G69/08 ,  C08G69/16 ,  C12P7/24 ,  C08G63/78 ,  C12P7/44 ,  C08G73/02 ,  C12N9/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US20190152887A1

公开(公告)日：2019-05-23

申请号：US16110973

申请日：2018-08-23

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC: C07C51/353 ,  C07C67/32 ,  C12P7/62 ,  C12P7/46 ,  C07C67/333 ,  C12P7/40 ,  C07C57/04 ,  C07C51/38 ,  C07C67/08

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

公开(公告)号：US20180030484A1

公开(公告)日：2018-02-01

申请号：US15488320

申请日：2017-04-14

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C08G69/26 ,  C08G63/66 ,  C08G63/12 ,  C08G63/06 ,  C12N9/10 ,  C12P19/02 ,  C12P7/24 ,  C08G18/32 ,  C12N9/88 ,  C12N9/04 ,  C12N9/06 ,  C07D207/12

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US09657316B2

公开(公告)日：2017-05-23

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/28 ,  C12P7/18 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C08G18/00 ,  C08G63/00 ,  C08G63/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P19/02

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US20170008828A1

公开(公告)日：2017-01-12

申请号：US15182245

申请日：2016-06-14

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC: C07C51/353 ,  C12P7/46 ,  C12P7/62 ,  C07C67/08

CPC classification number: C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

Abstract translation: 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

公开(公告)号：US20140329916A1

公开(公告)日：2014-11-06

申请号：US14107832

申请日：2013-12-16

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/02 ,  C12P7/24 ,  C12P19/02 ,  A23L1/221 ,  C12P7/28 ,  C12P9/00 ,  C12P13/00 ,  A23L1/054 ,  C12P7/44 ,  C12N15/70

CPC classification number: C12P17/10 ,  A23L29/065 ,  C08G63/78 ,  C08G69/08 ,  C08G69/16 ,  C08G73/0213 ,  C12N9/0006 ,  C12N15/52 ,  C12P7/24 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01244

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这样的还原当量可以用于提高微生物生物体如己二酸，6-氨基己酸，六亚甲基二胺或己内酰胺产生的有机化合物的产物产率。 本文还提供了使用这种生物体生产己二酸，6-氨基己酸酯，六亚甲基二胺或己内酰胺的方法。

公开(公告)号：US20140288254A1

公开(公告)日：2014-09-25

申请号：US14071339

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/42 ,  C08F120/06 ,  C12P7/24 ,  C08G63/664 ,  C12N15/63 ,  C12P7/40

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

公开(公告)号：US20140058056A1

公开(公告)日：2014-02-27

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物例如1,4-丁二醇（BDO）产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生BDO的方法。

公开(公告)号：US20240124904A1

公开(公告)日：2024-04-18

申请号：US18453987

申请日：2023-08-22

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Priti Pharkya ,  Tae Hoon Yang ,  Jungik Choi

IPC: C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42

CPC classification number: C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0036 ,  C12N9/0057 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12Y106/01002 ,  C12Y110/03 ,  C12Y207/0104 ,  C12Y301/00 ,  C12Y301/02 ,  C12Y301/02002 ,  C12Y304/21092 ,  C12Y401/01031 ,  C12Y401/01032

Abstract: −The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.