公开(公告)号：US11932895B2

公开(公告)日：2024-03-19

申请号：US17849571

申请日：2022-06-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyun Ju Oh ,  Youngsoon Um ,  Sun Mi Lee ,  Gyeongtaek Gong ,  Ja Kyong Ko

IPC: C12P7/06 ,  C12P7/16 ,  C12R1/145

CPC classification number: C12P7/06 ,  C12P7/16 ,  C12R2001/145

Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.

公开(公告)号：US11319556B2

公开(公告)日：2022-05-03

申请号：US16551335

申请日：2019-08-26

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Phuong Nguyen Hoang Tran ,  Youngsoon Um ,  Gyeongtaek Gong ,  Ja Kyong Ko ,  Yeonjung Lee

IPC: C12N15/90 ,  C12N1/16 ,  C12N15/81 ,  C12P7/06 ,  C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/92 ,  C12N15/11 ,  C12N9/22

Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.

公开(公告)号：US10913942B2

公开(公告)日：2021-02-09

申请号：US16204020

申请日：2018-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Sangdo Yook ,  Youngsoon Um ,  Gyeongtaek Gong ,  Jiwon Kim

IPC: C12N9/92 ,  C12N1/16 ,  C12P7/64 ,  C12N9/12 ,  C12N15/01 ,  C12N15/52 ,  C07K14/39

Abstract: Disclosed herein are a yeast strain capable of utilizing xylose as a carbon source and a method for producing lipids using the same. The yeast strain is obtained by adaptively evolving a wild-type yeast strain which cannot utilize xylose as a carbon source so that it can produce high density lipids and then transforming the adaptively evolved strain to obtain the ability to metabolize xylose. Since the strain does not have the xylose metabolic pathway based on oxidoreductase, it can produce biodiesel and biomaterials based on lipid and lignocellulosic biomass at a high yield without a problem of cofactor imbalance and can greatly improve the economic feasibility and sustainability of the production processes of biodiesel and biomaterials.

公开(公告)号：US10036046B2

公开(公告)日：2018-07-31

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Hyun Jeong Lee ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N1/20 ,  C12N15/70 ,  C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/6463 ,  C12Y101/01001 ,  C12Y401/01001 ,  Y02E50/13

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US09688976B2

公开(公告)日：2017-06-27

申请号：US15057551

申请日：2016-03-01

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Youngsoon Um ,  Jun Won Chwa ,  Gyeongtaek Gong

IPC: C12P7/28 ,  C12N15/09 ,  C12N9/88 ,  C12N9/00 ,  C12N9/10

CPC classification number: C12N9/88 ,  C12N9/13 ,  C12N9/93 ,  C12Y208/03009 ,  C12Y401/01004 ,  C12Y602/01001 ,  Y02P60/247

Abstract: Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

公开(公告)号：US09441230B2

公开(公告)日：2016-09-13

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min Woo ,  Youngsoon Um ,  Yunje Kim ,  Gyeong Taek Gong ,  Jung Seok Lee

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。