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    Method for producing dipeptides
    11.
    发明授权
    Method for producing dipeptides 失效
    二肽生产方法

    公开(公告)号：US08084227B2

    公开(公告)日：2011-12-27

    申请号：US11330076

    申请日：2006-01-12

    申请人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

    发明人： Kenzo Yokozeki ,  Isao Abe ,  Seiichi Hara

    IPC分类号： C12P21/06 ,  C12P1/00 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12P21/02

    摘要： The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.

    摘要翻译： 本发明提供了一种通过工业上有利且简单的途径以低成本获得的起始材料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。

    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME
    12.
    发明申请
    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME 有权
    生产用于其的丝氨酸衍生物和蛋白质的方法

    公开(公告)号：US20100317068A1

    公开(公告)日：2010-12-16

    申请号：US12836984

    申请日：2010-07-15

    申请人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    发明人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    IPC分类号： C12P13/06 ,  C07H21/00 ,  C12N9/10 ,  C12N1/21

    CPC分类号： C12N9/1029 ,  C12P13/04

    摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.

    摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。

    NOVEL PEPTIDE-FORMING ENZYME GENE
    13.
    发明申请
    NOVEL PEPTIDE-FORMING ENZYME GENE 有权
    新型肽形成酶基因

    公开(公告)号：US20100267082A1

    公开(公告)日：2010-10-21

    申请号：US12729656

    申请日：2010-03-23

    申请人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

    发明人： Seiichi Hara ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

    IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N1/21 ,  C07H21/04

    CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

    摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

    摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

    Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester
    16.
    发明授权
    Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
    制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法

    公开(公告)号：US07745172B2

    公开(公告)日：2010-06-29

    申请号：US11970203

    申请日：2008-01-07

    申请人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

    发明人： Kenzo Yokozeki ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

    IPC分类号： C12P21/06

    CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

    摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

    摘要翻译： 制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯（也称为α-L- - （取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L 提供了天竺葵酰-L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;产品名称：阿斯巴甜），容易地以高产率和低成本而不经过复合合成方法。 此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易且廉价且高产率的制备方法。 使用具有催化反应能力的酶或酶的物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯 其中L-苯丙氨酸对L-天冬氨酸-α，bgr-siester的“酯”位点不发生亲核攻击，但对其α-酯位点进行亲核攻击。

    NOVEL PEPTIDE-FORMING ENZYME GENE
    18.
    发明申请
    NOVEL PEPTIDE-FORMING ENZYME GENE 有权
    新型肽形成酶基因

    公开(公告)号：US20080166761A1

    公开(公告)日：2008-07-10

    申请号：US11841151

    申请日：2007-08-20

    申请人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

    发明人： Seiichi HARA ,  Kenzo Yokozeki ,  Isao Abe ,  Naoto Tonouchi ,  Yasuko Jojima

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N5/00

    CPC分类号： C12N9/00 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02

    摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

    摘要翻译： 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

    Transporter protein
    19.
    发明授权
    Transporter protein 失效
    转运蛋白

    公开(公告)号：US07320794B2

    公开(公告)日：2008-01-22

    申请号：US11276614

    申请日：2006-03-08

    申请人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    发明人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    IPC分类号： A61K39/00 ,  A61K39/38 ,  C12P1/00 ,  C12P21/06 ,  C07K1/00 ,  C07H21/04

    CPC分类号： C07K14/195

    摘要： A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to uptake hydantoin compounds is obtained by introducing and expressing the novel gene, called mhp, using gene recombination techniques.

    摘要翻译： 描述了具有转运乙内酰脲化合物的活性的新型蛋白质，以及表达该转运蛋白的重组体。 从微型液化酵母菌株AJ3912开始，发现了一种能够转运乙内酰脲化合物的蛋白质的新基因。 通过使用基因重组技术引入和表达新的基因，称为mhp，获得具有优异吸收乙内酰脲化合物能力的重组体。