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11.发明申请公开(公告)号：US20090023201A1
公开(公告)日：2009-01-22
申请号：US12175978
申请日：2008-07-18
申请人： Sadato Hongo , Takahiro Kokubo , Hideki Horiuchi , Koji Hashimoto , Jun Okada
发明人： Sadato Hongo , Takahiro Kokubo , Hideki Horiuchi , Koji Hashimoto , Jun Okada
IPC分类号： C12M1/00
CPC分类号： B01L3/502738 , B01L3/502 , B01L3/50273 , B01L7/52 , B01L2200/0621 , B01L2200/10 , B01L2200/12 , B01L2200/142 , B01L2300/06 , B01L2300/0627 , B01L2300/0645 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1827 , B01L2300/1883 , B01L2400/0487 , B01L2400/0655 , G01N2035/00366
摘要： In a nucleic acid detecting cassette in which a heating treatment chamber and a liquid delivery channel are constituted of a stationary member and flexible members, the heating treatment chamber is held by convex heating sections from both sides, whereby the heat treatment chamber and the liquid delivery channel are spatially separated from each other. Thus, it is possible to automatically execute a consistent process including nucleic acid amplification and other required treatments of the object sample as well as detection of a target nucleic acid.
摘要翻译： 在其中加热处理室和液体输送通道由固定部件和柔性部件构成的核酸检测盒中，加热处理室由两侧由凸形加热部保持，由此热处理室和液体输送 通道在空间上彼此分离。 因此，可以自动执行包括对象样品的核酸扩增和其他所需处理以及靶核酸的检测的一致的过程。
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公开(公告)号：US20090275028A1
公开(公告)日：2009-11-05
申请号：US12329898
申请日：2008-12-08
申请人： Naoko Nakamura , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko Nakamura , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6837 , C12Q2527/101 , C12Q2525/204 , C12Q2565/501
摘要： The present invention provides a method of detecting a target nucleic acid which includes a step of examining whether a washing step has been normally conducted. In an aspect of the invention, a monitoring nucleic acid probe to monitor the washing level is used. The probe shows a change in signal intensity by washing at a washing temperature changed in the optimum temperature range for washing the target nucleic acid and in a temperature range in the vicinity of the optimum temperature range for washing.
摘要翻译： 本发明提供了检测目标核酸的方法，其包括检查洗涤步骤是否正常进行的步骤。 在本发明的一个方面，使用监测洗涤水平的监测核酸探针。 探针通过在洗涤目标核酸的最适温度范围内和在用于洗涤的最佳温度范围附近的温度范围内改变的洗涤温度下洗涤来显示信号强度的变化。
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公开(公告)号：US07488581B2
公开(公告)日：2009-02-10
申请号：US11378453
申请日：2006-03-20
申请人： Naoko Nakamura , Koji Hashimoto
发明人： Naoko Nakamura , Koji Hashimoto
CPC分类号： C12Q1/6813
摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.
摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。
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公开(公告)号：US20070218464A1
公开(公告)日：2007-09-20
申请号：US11378453
申请日：2006-03-20
申请人： Naoko Nakamura , Koji Hashimoto
发明人： Naoko Nakamura , Koji Hashimoto
CPC分类号： C12Q1/6813
摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.
摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。
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公开(公告)号：US08673595B2
公开(公告)日：2014-03-18
申请号：US13340572
申请日：2011-12-29
申请人： Naoko Nakamura , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko Nakamura , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6809 , C12Q1/6853 , C12Q2525/161 , C12Q2525/301 , C12Q2537/143 , C12Q2563/179 , C12Q2565/514
摘要： One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.
摘要翻译： 一个实施例涉及分析多个样本的方法。 该方法包括使用第一引物和第二引物扩增多个样品，其中第一引物包含具有不同于样品的序列的标签序列，并且其中与独立反应体系中的第一引物成对配对的第二引物 对于各样品获得其中引入标签序列的扩增产物，混合在多个反应体系中获得的扩增产物，使得混合的扩增产物与固定在底物上的核酸探针反应，并检测杂交的量 已经发生了。
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公开(公告)号：US20120171673A1
公开(公告)日：2012-07-05
申请号：US13340572
申请日：2011-12-29
申请人： Naoko NAKAMURA , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko NAKAMURA , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6809 , C12Q1/6853 , C12Q2525/161 , C12Q2525/301 , C12Q2537/143 , C12Q2563/179 , C12Q2565/514
摘要： One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.
摘要翻译： 一个实施例涉及分析多个样本的方法。 该方法包括使用第一引物和第二引物扩增多个样品，其中第一引物包含具有不同于样品的序列的标签序列，并且其中与独立反应体系中的第一引物成对配对的第二引物 对于各样品获得其中引入标签序列的扩增产物，混合在多个反应体系中获得的扩增产物，使得混合的扩增产物与固定在底物上的核酸探针反应，并检测杂交的量 已经发生了。
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公开(公告)号：US07919252B2
公开(公告)日：2011-04-05
申请号：US12341295
申请日：2008-12-22
申请人： Naoko Nakamura , Koji Hashimoto
发明人： Naoko Nakamura , Koji Hashimoto
CPC分类号： C12Q1/6813
摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.
摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。
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公开(公告)号：US20090104621A1
公开(公告)日：2009-04-23
申请号：US12341295
申请日：2008-12-22
申请人： Naoko NAKAMURA , Koji Hashimoto
发明人： Naoko NAKAMURA , Koji Hashimoto
CPC分类号： C12Q1/6813
摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.
摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。
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公开(公告)号：US5972692A
公开(公告)日：1999-10-26
申请号：US886161
申请日：1997-06-30
申请人： Koji Hashimoto , Keiko Ito , Yoshio Ishimori
发明人： Koji Hashimoto , Keiko Ito , Yoshio Ishimori
CPC分类号： C12Q1/6834 , B01L7/525 , C12N15/1006 , C12Q1/6816 , C12Q1/6825 , G01N27/3276 , B01L2300/0645 , B01L2300/0819 , B01L3/50851 , B01L7/54
摘要： A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.
摘要翻译： 将具有与待检测基因互补的碱基序列的单链核酸探针固定在电极表面或光纤尖端上，使核酸探针与基因样品变性为单链形式， 然后检测与该基因杂交的核酸探针。 在该方法中，对于由核酸探针和基因样品构成的反应体系，添加能够特异性结合双链核酸并且以电化学或光学活性结合的双链核酸识别物质。 通过使用上述电极或光纤的电化学或光学测定来进行核酸探针的检测。 通过这种方法，即使在减少的时间内也可以以更高的灵敏度检测更基础的基因。
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公开(公告)号：US5776672A
公开(公告)日：1998-07-07
申请号：US167113
申请日：1993-12-16
申请人： Koji Hashimoto , Keiko Ito , Yoshio Ishimori , Masanori Gotoh
发明人： Koji Hashimoto , Keiko Ito , Yoshio Ishimori , Masanori Gotoh
CPC分类号： C12Q1/6834 , B01L7/525 , C12N15/1006 , C12Q1/6816 , C12Q1/6825 , G01N27/3276 , B01L2300/0645 , B01L2300/0819 , B01L3/50851 , B01L7/54
摘要： A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.
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