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公开(公告)号：US20070281307A1
公开(公告)日：2007-12-06
申请号：US11746722
申请日：2007-05-10
申请人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
发明人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
CPC分类号： B01D61/422 , B01D2325/20 , C12Q1/6806 , C12Q2565/629
摘要： A method of amplifying nucleic acid from blood, the method including performing an electrodialysis on a blood sample to reduce the ionic strength of the sample, and performing a Polymerase Chain Reaction (“PCR”) using the blood sample on which the electrodialysis is performed as a template.
摘要翻译： 一种从血液中扩增核酸的方法，所述方法包括对血液样品进行电渗析以降低样品的离子强度，并使用进行电渗析的血液样品进行聚合酶链式反应（“PCR”）， 一个模板
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12.发明申请Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法公开(公告)号：US20060154284A1
公开(公告)日：2006-07-13
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译： 提供了从含有核酸的样品中分离核酸的方法。 该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。
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公开(公告)号：US08986986B2
公开(公告)日：2015-03-24
申请号：US13284364
申请日：2011-10-28
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
摘要： A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.
摘要翻译： 一种裂解细胞和病毒中的至少一种的方法，所述方法包括：将包括细胞和病毒中的至少一种的样品与设置在第一室中的多个珠接触以获得组合 的样品和珠粒; 并搅拌样品和珠的组合以裂解细胞和病毒中的至少一种，其中在第一室中液体体积分数为0.6或更小，并且其中液体体积分数是通过将 第一室的液体体积相当于第一室的液体体积和第一室的空隙体积的总和的纯空隙体积。
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14.发明授权Method of amplifying nucleic acid from a cell using a nonplanar solid substrate 有权使用非平面固体底物从细胞中扩增核酸的方法公开(公告)号：US07919278B2
公开(公告)日：2011-04-05
申请号：US11841107
申请日：2007-08-20
申请人： Sung-young Jeong , Kyu-youn Hwang , Joon-ho Kim , Jung-im Han , Hun-joo Lee
发明人： Sung-young Jeong , Kyu-youn Hwang , Joon-ho Kim , Jung-im Han , Hun-joo Lee
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.
摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。
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公开(公告)号：US09044755B2
公开(公告)日：2015-06-02
申请号：US13176777
申请日：2011-07-06
申请人： Chin-sung Park , Won-jong Jung , Joon-ho Kim , Kak Namkoong , Kyu-youn Hwang , Won-seok Chung , Hee-kyun Lim , Sung-hong Kwon , Sun-ok Jung
发明人： Chin-sung Park , Won-jong Jung , Joon-ho Kim , Kak Namkoong , Kyu-youn Hwang , Won-seok Chung , Hee-kyun Lim , Sung-hong Kwon , Sun-ok Jung
CPC分类号： B01L7/52 , B01L3/502715 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0605 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/18 , B01L2400/0487 , B01L2400/06 , B01L2400/0655 , C12Q1/68 , G01N21/75
摘要： A gene analysis apparatus includes a sample preparation chip in which a polymerase chain reaction (“PCR”) sample is prepared, a PCR chip in which a PCR is performed on the PCR sample, and a package layer on which the sample preparation chip and the PCR chip are mounted. The package layer includes a channel through which a material flows from the sample preparation chip to the PCR chip. The sample preparation chip and the PCR chip are on a same side or on opposing sides of the package layer.
摘要翻译： 基因分析装置包括准备了聚合酶链式反应（“PCR”）样品的样品制备芯片，对PCR样品进行PCR的PCR芯片，以及将样品制备芯片和 PCR芯片安装。 封装层包括材料从样品制备芯片流向PCR芯片的通道。 样品制备芯片和PCR芯片在封装层的相同侧或相对侧上。
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公开(公告)号：US08895297B2
公开(公告)日：2014-11-25
申请号：US13492179
申请日：2012-06-08
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
CPC分类号： C12M47/06 , B01L3/502753 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , C12N1/06
摘要： A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber.
摘要翻译： 用于破坏电池的微型装置包括其中电池被破坏的第一室，被加压和减压的第二室，分离第一室和第二室的柔性膜，并且通过对第二室进行加压和减压而振动， 以及限制在第一室中的微单元，其中微单元破坏第一室中的单元。
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17.发明授权Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same 有权使用非平面固体底物分离微生物的方法和使用其分离微生物的装置公开(公告)号：US08557564B2
公开(公告)日：2013-10-15
申请号：US13325980
申请日：2011-12-14
申请人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim
发明人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim
IPC分类号： C12N1/02
摘要： Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material, and contacting the reacted sample with a means for capturing microorganisms.
摘要翻译： 提供了一种从样品中分离微生物的方法，包括使含有微生物的样品与交换材料上的无机物接触，使得样品与无机的交换材料反应，并使反应的样品与捕获微生物的装置接触。
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18.发明授权Microfluidic device and method for concentrating sample containing cells or viruses and lysing cells or viruses, and method of producing the microfluidic device 有权用于浓缩含有细胞或病毒的样品和裂解细胞或病毒的微流体装置和方法以及产生微流体装置的方法公开(公告)号：US07955842B2
公开(公告)日：2011-06-07
申请号：US11670002
申请日：2007-02-01
申请人： Hun-joo Lee , Joon-ho Kim , Sung-young Jeong , Kyu-youn Hwang
发明人： Hun-joo Lee , Joon-ho Kim , Sung-young Jeong , Kyu-youn Hwang
摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.
摘要翻译： 一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。 阴极室包括固体支撑体。 微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。
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19.发明授权Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法公开(公告)号：US07695678B2
公开(公告)日：2010-04-13
申请号：US12141894
申请日：2008-06-18
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译： 提供了从含有核酸的样品中分离核酸的方法。 该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。
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公开(公告)号：US20060166241A1
公开(公告)日：2006-07-27
申请号：US11325834
申请日：2006-01-05
申请人： Jeo-young Shim , Kyu-youn Hwang , Joon-ho Kim , Sung-ouk Jung , Kyu-tae Yoo , Joon-shik Park , Ji-na Namgoong
发明人： Jeo-young Shim , Kyu-youn Hwang , Joon-ho Kim , Sung-ouk Jung , Kyu-tae Yoo , Joon-shik Park , Ji-na Namgoong
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2563/173 , C12Q2523/308
摘要： Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids contained in the nucleic acid sample; cleaning the resultant structure where the nucleic acids are bound to the intercalator immobilized on the solid support; and eluting the nucleic acids with a second buffer solution.
摘要翻译： 提供了核酸分离单元和方法。 该方法包括将含芳族化合物的核酸嵌入剂固定在固体支持物上; 将含有要纯化的核酸样品的第一缓冲溶液与固定在固体支持物上的嵌入剂接触，以使嵌入剂与核酸样品中所含的核酸结合; 清洗所得结构，其中核酸与固定在固相载体上的嵌入剂结合; 并用第二缓冲溶液洗脱核酸。
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