公开(公告)号：US20240101999A1

公开(公告)日：2024-03-28

申请号：US18235205

申请日：2023-08-17

Applicant: Thermo Fisher Scientific GENEART GmbH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas POEHMERER ,  Phillip KUHN ,  Frank NOTKA ,  Andreas ZEIDLER ,  Korbinian HEIL ,  Axel TREFZER ,  Geir FONNUM ,  Federico KATZEN ,  Kristian ANDERSSON ,  Xiquan LIANG

IPC: C12N15/10 ,  B01L3/00 ,  B03C5/02 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B03C5/026 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C2201/26

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20140073738A1

公开(公告)日：2014-03-13

申请号：US14082336

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Grete I. MODAHL ,  Nini H. KJUS ,  Astrid E. MOLTEBERG ,  Diem TRAN ,  Jo AASERUD ,  Talha M. GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C08F8/32

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。

公开(公告)号：US20210155726A1

公开(公告)日：2021-05-27

申请号：US17165039

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofsloekken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem Thuy Thi TRAN ,  Jo AASERUD ,  M. Tahla GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C07F7/18 ,  C08F8/12 ,  C12Q1/686 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F6/24 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20190390018A1

公开(公告)日：2019-12-26

申请号：US16253141

申请日：2019-01-21

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene HUSABOE ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F220/70 ,  C08F8/12 ,  C08F220/58

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20190177787A1

公开(公告)日：2019-06-13

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  Alfred LUI ,  Guobing XIANG ,  Synne LARSEN

IPC: C12Q1/6874 ,  C08J7/14 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US20160326291A1

公开(公告)日：2016-11-10

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang YING ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C08F230/08

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20140073756A1

公开(公告)日：2014-03-13

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/18 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

公开(公告)号：US20170218095A1

公开(公告)日：2017-08-03

申请号：US15453750

申请日：2017-03-08

Applicant: LIFE TECHNOLOGIES AS

Inventor： Grete MODAHL ,  Geir FONNUM ,  Astrid MOLTEBERG ,  Silje LIEN ,  Nini KJUS

IPC: C08F2/22 ,  C08J9/20

CPC classification number: C08F2/22 ,  C08F8/30 ,  C08F12/08 ,  C08F212/36 ,  C08F257/02 ,  C08F2800/20 ,  C08J3/128 ,  C08J9/18 ,  C08J9/20 ,  C08J2325/08 ,  C12N11/08 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2998 ,  C08F212/08

Abstract: This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (CV) % of the particles, when measured by CPS disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. This invention also relates to monodisperse polymer particles for use as seed particles in the Ugelstad process.

公开(公告)号：US20230322996A1

公开(公告)日：2023-10-12

申请号：US18053066

申请日：2022-11-07

Applicant: LIFE TECHNOLOGIES AS

Inventor： Huey Wen OOI ,  Pontus LUNDBERG ,  Maxi-Lu BOESCHEN ,  Hilde ALMAASBAK ,  Tuva Holt HERENG ,  Tor Espen STAV-NORAAS ,  Ole LANDSVERK ,  Geir FONNUM

IPC: C08F283/04

CPC classification number: C08F283/04

Abstract: The invention relates to polymer-interaction molecule (e.g., antibodies) conjugates and uses of such conjugates for delivery of interaction molecules to cells. The invention also relates uses polymer-interaction molecule (e.g., antibodies) conjugates for eliciting cellular responses (e.g., induction of cell proliferation).

公开(公告)号：US20170306072A1

公开(公告)日：2017-10-26

申请号：US15647052

申请日：2017-07-11

Applicant: LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Lise LILJEROTH

IPC: C08F293/00 ,  C08F287/00 ,  C08L53/00 ,  C09D153/00

CPC classification number: C08F293/00 ,  C08F287/00 ,  C08F292/00 ,  C08F293/005 ,  C08F2438/01 ,  C08L53/005 ,  C09D153/005 ,  C08L2666/02

Abstract: Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.