公开(公告)号：US09985624B2

公开(公告)日：2018-05-29

申请号：US15049462

申请日：2016-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy Jordan ,  Todd Rearick

IPC: G06F1/06 ,  G06F1/26 ,  H03K17/16 ,  G01N27/414 ,  H03K19/003

CPC classification number: H03K17/162 ,  G01N27/4145 ,  G06F1/06 ,  G06F1/26 ,  H03K19/00346

Abstract: A system including a power supply and a clock circuitry to generate a plurality of clock signals. Each clock signal is synchronous with a primary clock signal. First, second, and third clock signals of the plurality of clock signals are asynchronous to each other. The system further includes a plurality of switches. Each switch of the plurality of switches is communicatively coupled to the power supply and the clock circuitry. A first switch of the plurality of switches receives the first clock signal, a second switch of the plurality of switches receives the second clock signal, and a third switch of the plurality of switches receives the third clock signal.

公开(公告)号：US09958415B2

公开(公告)日：2018-05-01

申请号：US14703752

申请日：2015-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Todd Rearick

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68

CPC classification number: G01N27/4145 ,  C12Q1/6874 ,  G01N27/4148

Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US09927393B2

公开(公告)日：2018-03-27

申请号：US14330756

申请日：2014-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Melville Davey ,  Mark Beauchemin

IPC: G01N27/416 ,  G01N27/414 ,  G06F19/22

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor may can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：US09515676B2

公开(公告)日：2016-12-06

申请号：US13754566

申请日：2013-01-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles Sugnet ,  Simon Cawley ,  Mohit Gupta ,  Iztok Marjanovic ,  Todd Rearick ,  Mark Beauchemin ,  Earl Hubbell

IPC: G06F19/24 ,  G11C17/00 ,  H03M7/30

CPC classification number: H03M7/30 ,  G06F19/24

Abstract: A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; and applying a second compression process to the waveform, the second compression process including a truncating of data corresponding to a portion of the waveform that is not related to a nucleotide incorporation component of the waveform.

Abstract translation: 压缩方法包括测量与传感器阵列上发生的化学事件相关联的波形，其中所述波形包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域; 对所述波形应用第一压缩处理，所述第一压缩处理包括所述波形的一个或多个部分中的一个或多个帧的平均值; 以及对所述波形应用第二压缩处理，所述第二压缩处理包括对应于与所述波形的核苷酸掺入成分无关的所述波形的一部分的数据的截断。

公开(公告)号：US11319587B2

公开(公告)日：2022-05-03

申请号：US16292087

申请日：2019-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James A. Ball

IPC: C12Q1/6874 ,  G01N27/414

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US11028438B2

公开(公告)日：2021-06-08

申请号：US16866653

申请日：2020-05-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim Johnson

IPC: C12Q1/6874 ,  G16B30/00

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors.

公开(公告)号：US10655175B2

公开(公告)日：2020-05-19

申请号：US16153898

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim Johnson

IPC: C12Q1/6874 ,  G16B30/00

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

公开(公告)号：US10578578B2

公开(公告)日：2020-03-03

申请号：US15351253

申请日：2016-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Jeremy Jordan ,  John Nobile ,  William Mileski ,  Chun Heen Ho

IPC: G01N33/48 ,  G01N27/414 ,  G01N31/20 ,  G05D23/19 ,  B01L3/00

Abstract: An apparatus for detecting chemical reactions may be provided. The apparatus may comprise a chemical detection device. The chemical detection device may include a chemical sensor, which may be mounted on the chemical detection device. The apparatus may further comprise a valve block. The valve block may fluidly couple a plurality of reagent containers to the chemical detection device. The apparatus may further comprise a heat exchanger and a controller. The controller may control a fluid connection between the valve block and the chemical detection device. The controller may be also configured to adjust a temperature of a selected reagent from the plurality of reagent containers via the heat exchanger. The temperature of the selected reagent may be adjusted prior to the reagent entering the chemical detection device.

公开(公告)号：US10451585B2

公开(公告)日：2019-10-22

申请号：US14926264

申请日：2015-10-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark Beauchemin

IPC: G01N33/48 ,  G01N33/50 ,  G01N27/414 ,  G01N27/27 ,  C12Q1/6869 ,  G16B30/00

Abstract: A method is provided to sample a sensor array. The method can include measuring a waveform associated with a chemical event occurring on the sensor array. The waveform can include at least one region associated with expected measured values and at least one region associated with unpredictable measured values. The method can also include applying a first frame averaging to the at least one region associated with the expected measured values. Here, a first number of frames can be included in the first frame averaging. Further, the method can include applying a second frame averaging to the at least one region associated with the unpredictable measured values, where a second number of frames can be included in the second frame averaging. The second number of frames can be less than the first number of frames.

公开(公告)号：US20190106745A1

公开(公告)日：2019-04-11

申请号：US16153898

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark MILGREW ,  Jonathan SCHULTZ ,  Chris PAPALIAS ,  Kim JOHNSON

IPC: C12Q1/6874 ,  G06F19/22

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.