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公开(公告)号：US20190119701A1
公开(公告)日：2019-04-25
申请号：US16124931
申请日：2018-09-07
Applicant: Life Technologies Corporation
Inventor： Xiquan LIANG , Robert Jason POTTER , Lansha PENG
IPC: C12N15/90 , C07K14/195 , C12N9/22
Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.
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公开(公告)号：US20160017394A1
公开(公告)日：2016-01-21
申请号：US14800384
申请日：2015-07-15
Applicant: Life Technologies Corporation
Inventor： Xiquan LIANG , Chang-Ho Baek , Lansha Peng , Federico Katzen
IPC: C12P19/34
CPC classification number: C12P19/34 , C12N15/10 , C12N15/1031 , C12N15/64 , C12N15/66
Abstract: The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.
Abstract translation: 本公开一般涉及用于将核酸分子装配到较大核酸分子中的组合物和方法。 还提供了用于使序列保真度高的核酸分子无缝连接的组合物和方法。
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公开(公告)号：US20240101999A1
公开(公告)日：2024-03-28
申请号：US18235205
申请日：2023-08-17
Applicant: Thermo Fisher Scientific GENEART GmbH , LIFE TECHNOLOGIES AS , LIFE TECHNOLOGIES CORPORATION
Inventor： Thomas POEHMERER , Phillip KUHN , Frank NOTKA , Andreas ZEIDLER , Korbinian HEIL , Axel TREFZER , Geir FONNUM , Federico KATZEN , Kristian ANDERSSON , Xiquan LIANG
CPC classification number: C12N15/101 , B01L3/502761 , B03C5/026 , C25B3/29 , C25B9/70 , C25B11/04 , C25B15/02 , B01J2219/00468 , B01J2219/005 , B01J2219/00653 , B01J2219/00695 , B01J2219/00722 , B01L3/502715 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0893 , B01L2400/046 , B01L2400/0487 , B03C2201/26
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US20220372522A1
公开(公告)日：2022-11-24
申请号：US17766998
申请日：2020-10-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xiquan LIANG , Pei-Zhong TANG , Robert POTTER
IPC: C12N15/90 , C12N9/22 , C12N15/11 , C07K14/245
Abstract: The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.
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Patent Agency Ranking
公开(公告)号：US20200080077A1
公开(公告)日：2020-03-12
申请号：US16536983
申请日：2019-08-09
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xiquan LIANG , Robert POTTER , Namritha RAVINDER , Shantanu KUMAR
IPC: C12N15/10 , C12N15/113 , C12N15/90
Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
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公开(公告)号：US20200032230A1
公开(公告)日：2020-01-30
申请号：US16534636
申请日：2019-08-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Robert POTTER , Jonathan CHESNUT , Xiquan LIANG
Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
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公开(公告)号：US20180245116A1
公开(公告)日：2018-08-30
申请号：US15873477
申请日：2018-01-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xiquan LIANG , Chang-Ho BAEK , Lansha PENG , Federico KATZEN
CPC classification number: C12P19/34 , C12N15/10 , C12N15/1031 , C12N15/64 , C12N15/66
Abstract: The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.
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公开(公告)号：US20180023075A1
公开(公告)日：2018-01-25
申请号：US15605586
申请日：2017-05-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xiquan LIANG , Robert POTTER , Namritha RAVINDER , Shantanu KUMAR
IPC: C12N15/10 , C12N15/113
CPC classification number: C12N15/1082 , A01K2217/07 , C12N15/102 , C12N15/1024 , C12N15/1034 , C12N15/113 , C12N15/907 , C12N2310/152 , C12N2310/153 , C12N2310/20 , C12N2310/533 , C12Q2600/156
Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
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公开(公告)号：US20170107539A1
公开(公告)日：2017-04-20
申请号：US15294586
申请日：2016-10-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xin YU , Xiquan LIANG , Xavier de MOLLERAT du JEU , Robert Jason POTTER
CPC classification number: C12N15/90 , A61K48/0033 , A61K48/005 , C12N9/22 , C12N15/88 , C12N2830/30 , C12Y301/00
Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.
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