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    Flat-field imaging system and methods of use
    11.
    发明授权
    Flat-field imaging system and methods of use 有权

    公开(公告)号：US11193872B2

    公开(公告)日：2021-12-07

    申请号：US16222664

    申请日：2018-12-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Michael Pallas ,  Haopeng Wang

    IPC: C12Q1/6816 ,  G01N15/04 ,  C12Q1/686

    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

    System and method for determining copies-per-unit-volume using PCR and flow control of droplets
    12.
    发明授权
    System and method for determining copies-per-unit-volume using PCR and flow control of droplets 有权

    公开(公告)号：US10557174B2

    公开(公告)日：2020-02-11

    申请号：US15080801

    申请日：2016-03-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    13.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：US20150322490A1

    公开(公告)日：2015-11-12

    申请号：US14796400

    申请日：2015-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许一个或多个靶序列的选择性扩增的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变体的存在。

    Compositions, methods, systems and kits for target nucleic acid enrichment
    14.
    发明授权
    Compositions, methods, systems and kits for target nucleic acid enrichment 有权
    用于靶核酸浓缩的组合物，方法，系统和试剂盒

    公开(公告)号：US09133510B2

    公开(公告)日：2015-09-15

    申请号：US14054618

    申请日：2013-10-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Steven Roman

    IPC: C07H21/00 ,  C12Q1/68

    CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

    Abstract translation: 本发明提供了可用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于包括核酸测序在内的各种下游过程。

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    15.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 有权

    公开(公告)号：US20250011760A1

    公开(公告)日：2025-01-09

    申请号：US18768211

    申请日：2024-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Sihong Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Guobin Luo

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B40/06 ,  C40B50/06

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    Compositions, methods, systems and kits for target nucleic acid enrichment
    16.
    发明授权
    Compositions, methods, systems and kits for target nucleic acid enrichment 有权

    公开(公告)号：US11130984B2

    公开(公告)日：2021-09-28

    申请号：US16846932

    申请日：2020-04-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Steven Roman

    IPC: C12Q1/6806 ,  C12Q1/6853 ,  C07H21/00

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

    Methods and compositions for multiplex PCR
    17.
    发明授权
    Methods and compositions for multiplex PCR 有权

    公开(公告)号：US10266881B2

    公开(公告)日：2019-04-23

    申请号：US14796400

    申请日：2015-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6806 ,  C07H21/04

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

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