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公开(公告)号：US20070119714A1
公开(公告)日：2007-05-31
申请号：US10595771
申请日：2004-11-10
申请人： Thomas Schnelle , Torsten Muller , Andre Homke
发明人： Thomas Schnelle , Torsten Muller , Andre Homke
IPC分类号： B03C5/02
CPC分类号： G01N15/1056 , B01L3/502761 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2400/0415 , B01L2400/0424 , G01N15/1456 , G01N15/1459 , G01N2015/105 , G01N2015/1495 , G01N2015/1497
摘要： Methods are described for analyzing at least one deformable object (O) in a suspension fluid, including the following steps: generation of an electric positioning field and positioning of the object (O) in a potential minimum of the positioning field, generation of an electric deformation field in such a way that a deformation force is exerted on the object (O), and detection of at least one property selected from the group including the dielectric, geometric and optical properties of the object (O), wherein the positioning field is generated in a compartment (12) of a fluidic microsystem (10) and the positioning of the object (O) takes place in a contactless manner in a freely suspended state. Measuring apparatuses for carrying out this method are also described.
摘要翻译： 描述了用于分析悬浮液中的至少一个可变形物体（O）的方法，包括以下步骤：产生电定位区域和将物体（O）定位在定位区域的潜在最小值中，产生电 变形场，使得变形力施加在物体（O）上，以及检测从包括物体（O）的电介质，几何和光学性质的组中选择的至少一种性质，其中定位场是 产生在流体微系统（10）的隔室（12）中，并且物体（O）的定位以无接触的方式在自由悬挂状态下进行。 还描述了用于执行该方法的测量装置。
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公开(公告)号：US20060152708A1
公开(公告)日：2006-07-13
申请号：US10544423
申请日：2004-02-04
申请人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Thomas Schnelle , Rudiger Meyer
发明人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Thomas Schnelle , Rudiger Meyer
IPC分类号： G01N21/00
CPC分类号： G01N15/1459 , C12M47/04 , G01N15/1056 , G01N15/12 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1037 , G01N2015/1081 , G01N2015/1477 , G01N2015/149 , G01N2015/1497 , G01N2035/00158
摘要： Disclosed is a method for measuring properties of particles that move through a fluidic microsystem, the particles being suspended in a liquid. According to the inventive method, a first parameter of a specific particle is first measured at a first test station (11). A second parameter of the particle is then measured at an interval from the first measurement at a second test station (12) which is spatially separated from the first test station (11), and the first and second parameters are jointly evaluated in a correlated manner, the first and second parameters being characteristic of different properties of the tested particle. The evaluation comprises a comparison of the first and second parameters with predetermined expected values. Another measurement is taken or manipulations are performed on the tested particle according to the result of the comparison. Also disclosed is a fluidic microsystem that is designed for implementing the inventive method.
摘要翻译： 公开了一种用于测量移动通过流体微系统的颗粒的性质的方法，所述颗粒悬浮在液体中。 根据本发明的方法，首先在第一测试台（11）测量特定颗粒的第一参数。 然后在与第一测试站（11）在空间上分离的第二测试站（12）以与第一测量的间隔的间隔测量颗粒的第二参数，并且以相关方式联合评估第一和第二参数 ，第一和第二参数是测试颗粒的不同性质的特征。 评估包括第一和第二参数与预定预期值的比较。 根据比较的结果，对测试的颗粒进行另一测量或操作。 还公开了被设计用于实施本发明方法的流体微系统。
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13.发明申请Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy 有权用于提供高分辨率显微镜的悬浮微物体三维成像的方法和装置公开(公告)号：US20060126921A1
公开(公告)日：2006-06-15
申请号：US10532147
申请日：2003-10-24
申请人： Spencer Shorte , Torsten Muller , Thomas Schnelle
发明人： Spencer Shorte , Torsten Muller , Thomas Schnelle
IPC分类号： G06K9/00
CPC分类号： G02B21/22 , G01N2015/144 , G01N2015/1445 , G01N2015/1452 , G02B21/367
摘要： A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.
摘要翻译： 一种用显微镜对至少一个物体进行高分辨率图像记录的方法，包括以下步骤：（a）将物体放置在布置在显微镜的光轴上的容器中，（b）产生至少两个第一数据 通过相对于显微镜的光轴具有至少两个不同取向来表示对象的每个对象的集合，其中通过相对于插座移动对象来提供对象的不同取向，以及（c）评估数据 用于获得定量三维信息。
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14.发明申请Fluidic microsystem comprising field-forming passivation layers provided on microelectrodes 有权流体微系统包括在微电极上提供的场形成钝化层公开(公告)号：US20060024802A1
公开(公告)日：2006-02-02
申请号：US10536674
申请日：2003-11-26
申请人： Torsten Muller , Thomas Schnelle
发明人： Torsten Muller , Thomas Schnelle
CPC分类号： B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B03C5/026
摘要： Described is a fluidic microsystem (100) comprising at least one channel (10) through which a particle suspension can flow; and first and second electrode devices (40, 60) which are arranged on first and second channel walls (21, 31) for generating electrical alternating-voltage fields in the channel (10); wherein the first electrode device (40) for field shaping in the channel comprises at least one first structure element (41, 51); and the second electrode device (60) comprises an area-like electrode layer (61) with a closed second electrode surface which comprises a second passivation layer (70); wherein the effective electrode surface of the first structure element (41, 51), of which element (41, 51) there is at least one, is smaller than the second electrode surface; and the second passivation layer (70) is a closed layer which completely covers the second electrode layer (61).
摘要翻译： 描述了流体微系统（100），其包括至少一个通道（10），颗粒悬浮液可以通过该通道流动; 以及布置在第一和第二通道壁（21,31）上的用于在通道（10）中产生电气交变电压场的第一和第二电极装置（40,60）。 其中用于所述通道中的场成形的所述第一电极装置（40）包括至少一个第一结构元件（41,51）; 并且第二电极装置（60）包括具有闭合的第二电极表面的区域状电极层（61），其包括第二钝化层（70）; 其中，所述元件（41,51）中至少有一个的所述第一结构元件（41,51）的有效电极表面小于所述第二电极表面; 并且所述第二钝化层（70）是完全覆盖所述第二电极层（61）的封闭层。
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公开(公告)号：US06801311B1
公开(公告)日：2004-10-05
申请号：US09889908
申请日：2001-12-12
IPC分类号： G01N1502
CPC分类号： B03C5/028 , B03C5/026 , G01N15/1031 , G01N15/1056 , G01N15/1484 , G01N2015/1006 , G01N2015/105 , G01N2015/1075 , G01N2015/1415 , G01N2015/1447 , G01N2015/1495
摘要： For object detection, particularly in fluidic microsystems, optical imaging of at least one resting or moving object (10) on a structured mask (20) with at least one segment from a flat section (80), in which the object (10) is located at least partially or temporarily and which has a characteristic dimension smaller than the dimension of the object (10) or its movement path, to a detector unit, detection of the quantity of light transmitted by the structured mask (20), and generation of a detector signal which has a predetermined relationship with the quantity of light, and evaluation of the detector signal in regard to the presence of the object (10), its position, its shape and/or the temporal change of the position are performed.
摘要翻译： 对于物体检测，特别是在流体微系统中，至少一个静止或移动的物体（10）在结构化掩模（20）上的光学成像，其具有来自平坦部分（80）的至少一个部分，其中物体（10） 至少部分地或临时地定位，并且具有比物体（10）或其移动路径的尺寸小的特征尺寸，到检测器单元，由结构化掩模（20）传输的光量的检测，以及 执行与光量具有预定关系的检测器信号，以及关于物体（10）的存在，其位置，形状和/或位置的时间变化的检测器信号的评估。
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公开(公告)号：US06610188B1
公开(公告)日：2003-08-26
申请号：US09308841
申请日：1999-07-30
IPC分类号： G01N2726
摘要： An electrode configuration for field cages, especially in microsystems, comprises a large number of electrodes in which an electrical potential can be applied to each end region through a feed region. The end region is arranged to form the field cage and inhomogeneous shielding fields outside the field cage. To reduce thermal convection, the feed region has a strip form whose width is substantially smaller than characteristic dimensions of the end region.
摘要翻译： 用于现场保持架的电极构造，特别是在微系统中，包括大量的电极，其中可以通过进料区域将电势施加到每个端部区域。 端部区域被布置成形成场笼和场笼外的不均匀屏蔽场。 为了减少热对流，进料区域具有宽度大大小于端部区域的特征尺寸的带状。
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公开(公告)号：US06492175B1
公开(公告)日：2002-12-10
申请号：US09868022
申请日：2001-08-06
IPC分类号： C12N1502
摘要： An electroporation and/or fusion treatment of microscopic objects occurs in a medium between at least two electrodes, with the electrodes being miniaturized electrodes in a microsystem with a channel structure which is set up for the flow-through of the medium with the objects.
摘要翻译： 在至少两个电极之间的介质中发生微观物体的电穿孔和/或融合处理，其中电极是具有通道结构的微系统中的小型化电极，其被设置用于具有物体的介质的流过。
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公开(公告)号：US08262883B2
公开(公告)日：2012-09-11
申请号：US10549886
申请日：2004-03-17
申请人： Torsten Müller , Thomas Schnelle , Rolf Hagedorn
发明人： Torsten Müller , Thomas Schnelle , Rolf Hagedorn
IPC分类号： B01D57/02
CPC分类号： B03C5/005
摘要： Methods and devices for the separation of particles (20, 21, 22) in a compartment (30) of a fluidic microsystem (100) are described, in which the movement of a liquid (10) in which particles (20, 21, 22) are suspended with a predetermined direction of flow through the compartment (30), and the generation of a deflecting potential in which at least a part of the particles (20, 21, 22) is moved relative to the liquid in a direction of deflection are envisaged, whereby further at least one focusing potential is generated, so that at least a part of the particles is moved opposite to the direction of deflection relative to the liquid by dielectrophoresis under the effect of high-frequency electrical fields, and guiding of particles with different electrical, magnetic or geometric properties into different flow areas (11, 12) in the liquid takes place.
摘要翻译： 描述了用于在流体微系统（100）的隔室（30）中分离颗粒（20,21,22）的方法和装置，其中液体（10）的运动，其中颗粒（20,21,22 ）以预定的流动方向悬挂通过隔室（30），并产生偏转电位，其中至少一部分颗粒（20,21,22）在偏转方向上相对于液体移动 被设想为进一步产生至少一个聚焦电位，使得至少一部分颗粒在高频电场的作用下通过介电电泳相对于液体的偏转方向移动，并且引导颗粒 在不同的流动区域（11,12）中发生不同的电，磁或几何特性。
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公开(公告)号：US08128797B2
公开(公告)日：2012-03-06
申请号：US12161267
申请日：2007-01-17
申请人： Torsten Müller , Thomas Schnelle
发明人： Torsten Müller , Thomas Schnelle
IPC分类号： B03C5/02
CPC分类号： C12N13/00 , B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2400/0421
摘要： The invention relates to an operating method for a microfluidic system, including the following steps: feeding of a carrier flow with particles (5) of a first particle type suspended therein into the microfluidic system; charging of a plurality of electrical field cages (1′, 1″) in the microfluidic system with the supplied particles (5) of the first particle type; the supplying of a carrier flow with particles (6) of a second particle type suspended therein into the microfluidic system; and charging the field cages (1′, 1″) in the microfluidic system with the supplied particles (6) of the second particle type in such a manner that a particle (5) of the first particle type and a particle (6) of the second particle type is present in at least one of the field cages (1′, 1′). The invention also relates to a corresponding microfluidic system.
摘要翻译： 本发明涉及一种用于微流体系统的操作方法，包括以下步骤：将载体流与悬浮于其中的第一颗粒类型的颗粒（5）进料到微流体系统中; 在微流体系统中与所提供的第一颗粒类型的颗粒（5）充电多个电场笼（1'，1“）; 将载体流与悬浮在其中的第二颗粒类型的颗粒（6）供应到微流体系统中; 以及将所述微流体系统中的所述场笼（1'，1“）与所提供的所述第二颗粒类型的颗粒（6）以使得所述第一颗粒类型的颗粒（5）和所述第一颗粒类型的颗粒（6） 第二粒子类型存在于至少一个场笼（1'，1'）中。 本发明还涉及相应的微流体系统。
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20.发明授权Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy 有权用于提供高分辨率显微镜的悬浮微物体三维成像的方法和装置公开(公告)号：US07738695B2
公开(公告)日：2010-06-15
申请号：US10532147
申请日：2003-10-24
申请人： Spencer Shorte , Torsten Müller , Thomas Schnelle
发明人： Spencer Shorte , Torsten Müller , Thomas Schnelle
IPC分类号： G06K9/00
CPC分类号： G02B21/22 , G01N2015/144 , G01N2015/1445 , G01N2015/1452 , G02B21/367
摘要： A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.
摘要翻译： 一种用显微镜对至少一个物体进行高分辨率图像记录的方法，包括以下步骤：（a）将物体放置在布置在显微镜的光轴上的容器中，（b）产生至少两个第一数据 通过相对于显微镜的光轴具有至少两个不同取向来表示对象的每个对象的集合，其中通过相对于插座移动对象来提供对象的不同取向，以及（c）评估数据 用于获得定量三维信息。
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