Cryo-Device and Associated Operational Method
    1.
    发明申请
    Cryo-Device and Associated Operational Method 有权
    冷冻设备及相关操作方法

    公开(公告)号:US20070267419A1

    公开(公告)日:2007-11-22

    申请号:US11576406

    申请日:2005-09-30

    IPC分类号: F25D3/10

    摘要: The invention relates to a cryoapparatus for freezing and/or thawing a sample (1), especially in the cryopreservation of a biological sample (1), with a coolable cooling space (3) and with a sample container (10) arranged in the cooling space (3) for temporarily receiving the sample (1) when freezing or thawing the sample (1). It is suggested that the sample container (10) can be tempered separately from the cooling space. Furthermore, the invention comprises an associated operating method.

    摘要翻译: 本发明涉及一种用于冷冻和/或解冻样品(1)的冷冻装置,特别是在具有可冷却冷却空间(3)的冷冻保存中的生物样品(1)的冷冻保存以及布置在冷却中的样品容器(10) 用于在冷冻或解冻样品(1)时临时接收样品(1)的空间(3)。 建议将样品容器(10)与冷却空间分开回火。 此外,本发明包括相关的操作方法。

    Method and devices for treating individual biological cells
    2.
    发明授权
    Method and devices for treating individual biological cells 有权
    用于治疗个体生物细胞的方法和装置

    公开(公告)号:US08283132B2

    公开(公告)日:2012-10-09

    申请号:US11718149

    申请日:2005-11-02

    IPC分类号: C12Q1/02 C12N5/00 C12N15/02

    CPC分类号: C12M35/00 C12M35/04

    摘要: The invention relates to a method for treating a biological cell (1) including a cytoskeleton (3) enveloped by a cell membrane (2). The method includes the following steps: the biological cell (1) and a tool (10) are mutually oriented such that the tool (10) comes into contact with the biological cell (1); the tool (10) and the biological cell (1) are displaced in relation to each other; and a gap is formed in the molecular composite of the cell membrane (2) of the biological cell (1). During the displacement of the tool (10), the cytoskeleton (3) of the biological cell (1) has a state of equilibrium. The invention also relates to a cell manipulator for carrying out the inventive method.

    摘要翻译: 本发明涉及一种用于处理包括细胞膜(2)包围的细胞骨架(3)的生物细胞(1)的方法。 该方法包括以下步骤:生物细胞(1)和工具(10)相互取向,使得工具(10)与生物细胞(1)接触; 工具(10)和生物细胞(1)相对于彼此移位; 并且在生物细胞(1)的细胞膜(2)的分子复合物中形成间隙。 在工具(10)移位期间,生物细胞(1)的细胞骨架(3)具有平衡状态。 本发明还涉及用于实施本发明的方法的细胞操纵器。

    Method and device for a accomodating samples on cryosubstrates
    6.
    发明授权
    Method and device for a accomodating samples on cryosubstrates 有权
    用于在低温底物上容纳样品的方法和装置

    公开(公告)号:US06646238B1

    公开(公告)日:2003-11-11

    申请号:US10009911

    申请日:2002-03-19

    IPC分类号: H05B306

    CPC分类号: G01N1/42

    摘要: For sample picking on a cryosubstrate, on which multiple cryopreserved samples are each positioned at preselected sample positions, individual samples are selectively separated mechanically or thermally from the cryosubstrate and transferred to a target substrate.

    摘要翻译: 对于低温基板上的样品采集,多个冷冻保存样品各自定位在预先选定的样品位置,单独的样品选择性地与冷冻底物分离或分离,并转移到靶基质。

    Method and device for measuring, calibrating and using laser tweezers
    8.
    发明授权
    Method and device for measuring, calibrating and using laser tweezers 失效
    用于测量,校准和使用激光镊子的方法和装置

    公开(公告)号:US06991906B1

    公开(公告)日:2006-01-31

    申请号:US09582609

    申请日:1998-12-21

    IPC分类号: G01N33/53 G01N33/543

    CPC分类号: H05H3/04 G21K1/006

    摘要: To measure or exert optically-induced forces on at least one particle in the focus of an optical cage, the following steps are taken: a) the focus is positioned in a microelectrode arrangement with a three-dimensional electrical field that has a field gradient which forms an electrical capture area, and the focus is at a distance from the capture are and b) the amplitude of the electrical field, the light power of the light beam forming the optical cage, and/or the distance of the capture area from the focus are varied to detect which varied field property moves the particle from the focus to the capture area or vice versa, or at least to temporarily move the particle into the capture area.

    摘要翻译: 为了在光学保持架的焦点的至少一个颗粒上测量或施加光学诱导的力,采取以下步骤:a)焦点位于具有场梯度的三维电场的微电极布置中, 形成电捕获区域,并且聚焦距离捕获的距离为b)电场的幅度,形成光学笼的光束的光功率和/或捕获区域距离 改变焦点以检测哪个变化的场性质将粒子从焦点移动到捕获区域,反之亦然,或至少临时将颗粒移动到捕获区域中。

    Method and device for manipulating microparticles in fluid flows
    9.
    发明授权
    Method and device for manipulating microparticles in fluid flows 有权
    用于操纵流体流中微粒的方法和装置

    公开(公告)号:US06727451B1

    公开(公告)日:2004-04-27

    申请号:US09673034

    申请日:2000-11-30

    IPC分类号: B01D5702

    CPC分类号: B03C5/026 C12M47/04

    摘要: To manipulate microparticles in a fluid that intersects a first channel or several first channels as a stream, one or more microparticles (14) are exposed to electrical field barriers that change their direction from the direction of flow toward the edge of the flow to a lateral hole (17) of the respective first channel. As a result, microparticles can be moved back and forth between streaming fluids. Preferred applications include treatment, separating, sorting or confinement procedures.

    摘要翻译: 为了操纵在与第一通道或多个第一通道相交的流体中的微粒作为流,一个或多个微粒暴露于电场屏障,其将其方向从流动方向朝向流动边缘改变为侧向 (17)。 结果,微粒可以在流动流体之间来回移动。 优选的应用包括处理,分离,分选或限制程序。

    Method and device for manipulating particles in microsystems
    10.
    发明授权
    Method and device for manipulating particles in microsystems 失效
    在微系统中操纵粒子的方法和装置

    公开(公告)号:US06465225B1

    公开(公告)日:2002-10-15

    申请号:US09720219

    申请日:2001-02-22

    IPC分类号: C12N1300

    摘要: For manipulation of particles in a fluidic microsystem (15) in which the particles are moved in a predetermined reference direction in a suspension liquid, the microsystem (15) is closed off at least at its end (17a, 17b) pointing to the reference direction. The particles move under the influence of centrifugal forces and/or gravitational forces in the suspension liquid which is stationary in relation to the microsystem (15), with the centrifugal forces and/or gravitational forces essentially extending parallel to the reference direction. Furthermore, the particles in the microsystem (15) are exposed to deflection forces whose direction differs from that of the reference direction. (FIG. 1).

    摘要翻译: 为了在流体微系统(15)中处理颗粒,其中颗粒在悬浮液体中以预定的参考方向移动,微系统(15)至少在其指向参考方向的端部(17a,17b)处被封闭 。 在离心力和/或重力作用下,颗粒在离心力和/或重力基本上平行于参考方向延伸的悬浮液中相对于微系统(15)静止的运动。 此外,微系统(15)中的颗粒暴露于方向不同于参考方向的偏转力。 (图。1)。