摘要:
The invention relates to a cryoapparatus for freezing and/or thawing a sample (1), especially in the cryopreservation of a biological sample (1), with a coolable cooling space (3) and with a sample container (10) arranged in the cooling space (3) for temporarily receiving the sample (1) when freezing or thawing the sample (1). It is suggested that the sample container (10) can be tempered separately from the cooling space. Furthermore, the invention comprises an associated operating method.
摘要:
The invention relates to a method for treating a biological cell (1) including a cytoskeleton (3) enveloped by a cell membrane (2). The method includes the following steps: the biological cell (1) and a tool (10) are mutually oriented such that the tool (10) comes into contact with the biological cell (1); the tool (10) and the biological cell (1) are displaced in relation to each other; and a gap is formed in the molecular composite of the cell membrane (2) of the biological cell (1). During the displacement of the tool (10), the cytoskeleton (3) of the biological cell (1) has a state of equilibrium. The invention also relates to a cell manipulator for carrying out the inventive method.
摘要:
The invention relates to a sample receiving device (100), particularly for the cryoconservation of at least one sample, including a bundle (10) consisting of a plurality of hose-shaped flexible sample chambers (11, 12, . . . ) and a holding device (20) with which the bundle (10) of the sample chambers is joined. The holding device (20) has a plurality of holding frames (21, 22, . . . ) which are arranged in a longitudinal direction of the bundle (10).
摘要:
A microsystem adapted for dielectrophoretic manipulation of particles in a suspension liquid wherein the microsystem has a channel with channel walls and a longitudinal extension. An electrode arrangement is present which has at least one microelectrode on at least one of the channel walls. This acts to generate a field barrier which crosses the channel at least partly. The microelectrode has a band-shape or has a multitude of straight electrode sections connected to each other. The band-shape has a predetermined curvature or the straight electrode sections are arranged with predetermined different angles so that the field barrier has a predetermined curvature relative to the longitudinal extension of the channel.
摘要:
With a method for cryo-preservation, at least one specimen is arranged on a storage substrate and specimen data, which are characteristic for features of the specimen, are stored at specific positions. Also, a storage substrate for cryo-preservation with such a method is described.
摘要:
For sample picking on a cryosubstrate, on which multiple cryopreserved samples are each positioned at preselected sample positions, individual samples are selectively separated mechanically or thermally from the cryosubstrate and transferred to a target substrate.
摘要:
The invention relates to methods for testing substances using multicellular in vitro test systems, in particular systems that resemble organs, and to devices and kits for carrying out said methods.
摘要:
To measure or exert optically-induced forces on at least one particle in the focus of an optical cage, the following steps are taken: a) the focus is positioned in a microelectrode arrangement with a three-dimensional electrical field that has a field gradient which forms an electrical capture area, and the focus is at a distance from the capture are and b) the amplitude of the electrical field, the light power of the light beam forming the optical cage, and/or the distance of the capture area from the focus are varied to detect which varied field property moves the particle from the focus to the capture area or vice versa, or at least to temporarily move the particle into the capture area.
摘要:
To manipulate microparticles in a fluid that intersects a first channel or several first channels as a stream, one or more microparticles (14) are exposed to electrical field barriers that change their direction from the direction of flow toward the edge of the flow to a lateral hole (17) of the respective first channel. As a result, microparticles can be moved back and forth between streaming fluids. Preferred applications include treatment, separating, sorting or confinement procedures.
摘要:
For manipulation of particles in a fluidic microsystem (15) in which the particles are moved in a predetermined reference direction in a suspension liquid, the microsystem (15) is closed off at least at its end (17a, 17b) pointing to the reference direction. The particles move under the influence of centrifugal forces and/or gravitational forces in the suspension liquid which is stationary in relation to the microsystem (15), with the centrifugal forces and/or gravitational forces essentially extending parallel to the reference direction. Furthermore, the particles in the microsystem (15) are exposed to deflection forces whose direction differs from that of the reference direction. (FIG. 1).