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公开(公告)号:US06368843B1
公开(公告)日:2002-04-09
申请号:US09694531
申请日:2000-10-23
申请人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号: C12N926
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译: 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro(NLNSRVP)的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可以衍生自芽孢杆菌属物种。
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公开(公告)号:US06329185B1
公开(公告)日:2001-12-11
申请号:US09129033
申请日:1998-08-04
IPC分类号: C12P2106
CPC分类号: C12Y302/01089 , A23K20/189 , A23L2/62 , A23L2/84 , A23L29/06 , C12G1/0203 , C12N9/2468 , C12N9/2471 , C12Y302/01023 , D21C5/005
摘要: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme composition comprising the enzyme, and the use of the enzyme and enzyme composition for a number of industrial applications.
摘要翻译: 本发明涉及具有半乳聚糖酶活性的酶,编码该酶的DNA构建体,该酶的制备方法,包含该酶的酶组合物以及该酶和酶组合物在许多工业应用中的用途。
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公开(公告)号:US06190905B1
公开(公告)日:2001-02-20
申请号:US09408257
申请日:1999-09-29
申请人: Henrik Dalbøge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
发明人: Henrik Dalbøge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
IPC分类号: D06M1600
CPC分类号: C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要: An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO: 1 or 2. Methods are described for using the protease compositions in reducing vescosity, cleaning contact lenses, baking, and preparing animal feed.
摘要翻译: 在4-7的pH下显示出蛋白酶活性的分离和纯化的酶,其在5%过氧化氢中显示蛋白酶,并且由与SEQ ID NO:1或2所示的DNA序列杂交的DNA序列编码。描述方法 用于使用蛋白酶组合物减少紫外线,清洁隐形眼镜,烘烤和制备动物饲料。
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公开(公告)号:US06187580B1
公开(公告)日:2001-02-13
申请号:US09198955
申请日:1998-11-24
申请人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号: C12N988
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.
摘要翻译: 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro(NLNSRVP)的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可从芽孢杆菌属物种衍生。
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公开(公告)号:US06159718A
公开(公告)日:2000-12-12
申请号:US087194
申请日:1998-05-29
申请人: Henrik Dalboege , Lene Nonboe Andersen , Lene Venke Kofoed , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Torben Halkier
发明人: Henrik Dalboege , Lene Nonboe Andersen , Lene Venke Kofoed , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Torben Halkier
CPC分类号: C12G1/0203 , C12N9/2402 , C12Y302/01015
摘要: An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.
摘要翻译: 显示多聚半乳糖醛酸酶活性的酶,该酶与针对源自Aspergillus aculeatus的纯化多聚半乳糖醛酸酶CBS 101.43产生的抗体具有免疫反应性。 该酶可以通过重组DNA技术产生并且可以用于植物细胞壁的降解,例如在葡萄酒和果汁生产中。
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公开(公告)号:US5885819A
公开(公告)日:1999-03-23
申请号:US902655
申请日:1997-07-30
申请人: Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
发明人: Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
IPC分类号: A21D8/04 , A23K1/165 , A23K1/18 , A23L1/03 , A23L1/105 , A23L2/04 , C12C5/00 , C12C7/28 , C12G1/00 , C12G1/022 , C12N9/24 , C12N15/56 , D21C5/00 , C07H21/04 , C12N1/22 , C12N15/00
CPC分类号: C12Y302/01032 , A21D8/042 , A23K20/189 , A23K50/75 , A23L2/04 , A23L29/06 , A23L7/107 , C12C5/004 , C12C5/006 , C12C7/28 , C12G1/00 , C12G1/0203 , C12N9/2482 , C12Y302/01008 , D21C5/005
摘要: An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components e.g. in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.
摘要翻译: 显示出木聚糖酶活性的酶,该酶与针对源自Aspergillus aculeatus,CBS 101.43的纯化木聚糖酶产生的抗体具有免疫反应性。 该酶可用于降解植物细胞壁组分,例如 在制备饲料时,在烘烤中,在造纸和纸浆工业中,并将小麦分离成淀粉和面筋。
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公开(公告)号:US5871966A
公开(公告)日:1999-02-16
申请号:US737526
申请日:1996-11-08
申请人: Lene Venke Kofod , Lene Nonboe Andersen , Markus Sakari Kauppinen , Stephan Christgau , Henrik Dalb.o slashed.ge , Hans Sejr Olsen , Jens Breinholt
发明人: Lene Venke Kofod , Lene Nonboe Andersen , Markus Sakari Kauppinen , Stephan Christgau , Henrik Dalb.o slashed.ge , Hans Sejr Olsen , Jens Breinholt
IPC分类号: C12N15/55 , A21D8/04 , A61K8/66 , A61Q11/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N9/42 , C12P21/02 , D06M16/00 , C12P19/44 , C08B1/00 , C12N9/20 , C12N9/24
CPC分类号: C12Y302/01006 , A21D8/042 , A61K8/66 , A61Q11/00 , C11D3/38645 , C12N9/244 , D06M16/003
摘要: A partial amino acid sequence of an endo-.beta.-1,4-glucanase obtainable by means of Aspergillus aculeatus is described, and also corresponding recombinant DNA sequences, vectors and transformed hosts. Use of the endo-.beta.- 1,4-glucanase or a pectinase preparation enriched with the endo-.beta.-1,4-glucanase for degradation or modification of plant cell walls is described.
摘要翻译: PCT No.PCT / DK95 / 00188 Sec。 371日期:1996年12月12日 102(e)日期1996年12月16日PCT提交1995年5月11日PCT公布。 出版物WO95 / 31533 日期:1995年11月23日描述了通过Aspergillus aculeatus获得的内切-β-1,4-葡聚糖酶的部分氨基酸序列,以及相应的重组DNA序列,载体和转化的宿主。 描述了内源β-1,4-葡聚糖酶或富含内切-β-1,4-葡聚糖酶的果胶酶制剂用于降解或修饰植物细胞壁的用途。
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公开(公告)号:US6080567A
公开(公告)日:2000-06-27
申请号:US116622
申请日:1998-07-16
申请人: Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
发明人: Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
IPC分类号: A21D8/04 , A23K1/165 , A23K1/18 , A23L1/03 , A23L1/105 , A23L2/04 , C12C5/00 , C12C7/28 , C12G1/00 , C12G1/022 , C12N9/24 , C12N15/56 , D21C5/00
CPC分类号: C12Y302/01032 , A21D8/042 , A23K20/189 , A23K50/75 , A23L2/04 , A23L29/06 , A23L7/107 , C12C5/004 , C12C5/006 , C12C7/28 , C12G1/00 , C12G1/0203 , C12N9/2482 , C12Y302/01008 , D21C5/005
摘要: An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components, e.g., in the preparation of feed, in baking, in the paper and pulp industry, and in connection with separation of wheat into starch and gluten.
摘要翻译: 表现出木聚糖酶活性的酶,该酶与针对源自Aspergillus aculeatus,CBS 101.43的纯化木聚糖酶产生的抗体具有免疫反应性。 该酶可用于降解植物细胞壁组分,例如在制备饲料,烘焙中,纸和纸浆工业中,以及将小麦分离成淀粉和谷蛋白。
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公开(公告)号:US5811291A
公开(公告)日:1998-09-22
申请号:US522229
申请日:1995-09-25
申请人: Lene Venke Kofod , Lene Nonboe Andersen , Henrik Dalb.o slashed.ge , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Claus Christophersen , Per Munk Nielsen , Alphons Gerar Joseph Voragen , Hendrik Arie Schols
发明人: Lene Venke Kofod , Lene Nonboe Andersen , Henrik Dalb.o slashed.ge , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Claus Christophersen , Per Munk Nielsen , Alphons Gerar Joseph Voragen , Hendrik Arie Schols
IPC分类号: A23K20/189 , A23L2/84 , A23L29/00 , C12N9/24 , C12N9/26 , C12N15/09 , C12N15/56 , C12R1/66 , C13B10/08 , C13K13/00 , C08B1/00 , C08B30/04
CPC分类号: C13B10/083 , A23K20/189 , A23L2/84 , A23L29/06 , C12N9/2402 , C12Y302/01171 , C13K13/00
摘要: An enzyme exhibiting rhamnogalacturonase activity, capable of cleaving a rhamnogalacturonan backbone in such a manner that galacturonic acids are left as the non-reducing ends, and which exhibits activity on hairy regions from a soy bean material and/or on saponified hairy regions from a sugar beet material. The enzyme has the amino acid sequence of SEQ ID NO:2 and is encoded by the DNA sequence of SEQ ID NO:1.
摘要翻译: PCT No.PCT / DK94 / 00097 Sec。 371 1995年9月25日第 102(e)1995年9月25日PCT PCT 1994年3月4日PCT公布。 出版物WO94 / 20612 PCT 日期1994年9月15日一种表现出鼠李糖半乳糖醛酸酶活性的酶,其能够以半乳糖醛酸作为非还原性末端的方式切割鼠李糖半乳糖醛酸骨架,并且在大豆材料和/或皂化的毛发区域上显示活性 来自甜菜料的毛状区域。 该酶具有SEQ ID NO:2的氨基酸序列,由SEQ ID NO:1的DNA序列编码。
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公开(公告)号:US5770406A
公开(公告)日:1998-06-23
申请号:US745977
申请日:1996-11-08
申请人: Lene Venke Kofod , Lene Nonboe Andersen , Markus Sakari Kauppinen , Stephan Christgau , Henrik Dalb.o slashed.ge , Hans Sejr Olsen
发明人: Lene Venke Kofod , Lene Nonboe Andersen , Markus Sakari Kauppinen , Stephan Christgau , Henrik Dalb.o slashed.ge , Hans Sejr Olsen
IPC分类号: C12G1/00 , A23L2/52 , C12N1/14 , C12N9/24 , C12N15/00 , C12N15/09 , C12P21/00 , C12R1/685 , C12R1/69 , C12R1/885 , C12P19/44 , C12N1/00 , D21C1/00
CPC分类号: C12Y302/01075 , C12N9/2402 , Y10S435/945
摘要: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.
摘要翻译: 本发明涉及由DNA序列编码的β-(1-6) - 内葡聚糖酶活性的酶,该DNA序列a)包含SEQ ID No.3所示的DNA序列,或b)包含DNA的类似物 序列号3所示的序列,其i)与SEQ ID No.3所示的DNA序列同源,和/或ii)与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交, 和/或iii)编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-(1-6) - 葡聚糖酶,如SEQ ID No.4所示,源自哈茨木霉,CBS 243.71。 本发明还涉及编码该酶的DNA构建体,该酶的制备方法,含该酶的酶制剂,以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。
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