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    DELIVERY OF MATERIALS TO ANUCLEATE CELLS
    12.
    发明申请
    DELIVERY OF MATERIALS TO ANUCLEATE CELLS 审中-公开

    公开(公告)号：US20180201889A1

    公开(公告)日：2018-07-19

    申请号：US15865901

    申请日：2018-01-09

    Applicant: Massachusetts Institute of Technology

    Inventor： Armon R. Sharei ,  Klavs F. Jensen ,  James Robbins Abshire ,  Jacquin Clarence Niles

    IPC: C12M3/06 ,  C12M1/42 ,  C12N5/078 ,  A61K35/18

    CPC classification number: C12M23/16 ,  A61K35/18 ,  C12M35/04 ,  C12N5/0641 ,  C12N5/0644 ,  C12N15/87 ,  C12N2521/00 ,  C12N2527/00

    Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

    Intracellular Delivery
    13.
    发明申请
    Intracellular Delivery 审中-公开
    胞内输送

    公开(公告)号：US20140287509A1

    公开(公告)日：2014-09-25

    申请号：US14352354

    申请日：2012-10-17

    Applicant: Massachusetts Institute of Technology

    Inventor： Armon R. Sharei ,  Andrea Adamo ,  Robert S. Langer ,  Klavs F. Jensen

    IPC: C12N5/071

    Abstract: A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.

    Abstract translation: 一种用于引起细胞膜中的扰动的微流体系统，该系统包括限定内腔的微流体通道，并且被配置为使得悬浮在缓冲液中的细胞可以通过其中，其中微流体通道包括细胞变形收缩部，其中直径 收缩是细胞直径的函数。

    Delivery of materials to anucleate cells
    14.
    发明授权
    Delivery of materials to anucleate cells 有权

    公开(公告)号：US11299698B2

    公开(公告)日：2022-04-12

    申请号：US15865901

    申请日：2018-01-09

    Applicant: Massachusetts Institute of Technology

    Inventor： Armon R. Sharei ,  Klavs F. Jensen ,  James Robbins Abshire ,  Jacquin Clarence Niles

    IPC: C12N15/87 ,  C12N5/078 ,  A61K35/18 ,  C12M3/06 ,  C12M1/42

    Abstract: The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

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