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公开(公告)号：US20210170411A1
公开(公告)日：2021-06-10
申请号：US17075116
申请日：2020-10-20
发明人： Armon R. Sharei , Viktor A. Adalsteinsson , Nahyun Cho , Robert S. Langer , J. Christopher Love , Klavs F. Jensen
IPC分类号： B01L3/00 , C12Q1/04 , C12N5/09 , G01N15/14 , G01N33/574 , C12N5/078 , B82Y30/00 , G01N15/10 , G01N15/00 , G01N1/30
摘要： Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.
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公开(公告)号：US20200277566A1
公开(公告)日：2020-09-03
申请号：US16818021
申请日：2020-03-13
摘要： A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.
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公开(公告)号：US20220091099A1
公开(公告)日：2022-03-24
申请号：US17404286
申请日：2021-08-17
IPC分类号： G01N33/50 , G01N33/487 , C12N15/87
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.
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公开(公告)号：US10526573B2
公开(公告)日：2020-01-07
申请号：US15526517
申请日：2015-11-13
摘要： A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described.
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公开(公告)号：US11806714B2
公开(公告)日：2023-11-07
申请号：US17075116
申请日：2020-10-20
发明人： Armon R. Sharei , Viktor A. Adalsteinsson , Nahyun Cho , Robert S. Langer , J. Christopher Love , Klavs F. Jensen
IPC分类号： B01L3/00 , C12N5/09 , C12N5/078 , G01N15/14 , C12Q1/04 , G01N33/574 , G01N1/30 , G01N15/10 , B82Y30/00 , C12M3/06 , G01N15/00
CPC分类号： B01L3/502761 , C12N5/0634 , C12N5/0693 , C12Q1/04 , G01N15/1459 , G01N15/1484 , G01N33/574 , B01L2200/0652 , B01L2300/08 , B01L2400/0487 , B82Y30/00 , C12M23/16 , G01N1/30 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081
摘要： Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.
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公开(公告)号：US11125739B2
公开(公告)日：2021-09-21
申请号：US15542892
申请日：2016-01-12
IPC分类号： C12N15/87 , G01N33/50 , G01N33/487 , C12Q1/00
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.
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公开(公告)号：US11111472B2
公开(公告)日：2021-09-07
申请号：US15523142
申请日：2015-10-30
发明人： Armon R. Sharei , Shirley Mao , George Hartoularos , Sophia Liu , Megan Heimann , Pamela Basto , Gregory Szeto , Siddharth Jhunjhunwala , Darrell Irvine , Robert S. Langer , Klavs F. Jensen , Ulrich H. Von Andrian
IPC分类号： C12M1/42 , C12M3/06 , A61K39/00 , A61K45/06 , A61K49/00 , C12N15/87 , G01N33/50 , C12N5/0781 , C12N5/0783 , C12N5/0784
摘要： A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.
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公开(公告)号：US12130281B2
公开(公告)日：2024-10-29
申请号：US17404286
申请日：2021-08-17
IPC分类号： G01N33/487 , C12N15/87 , G01N33/50 , C12Q1/00
CPC分类号： G01N33/5002 , C12N15/87 , G01N33/48721 , C12Q1/00
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.
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公开(公告)号：US20230130686A1
公开(公告)日：2023-04-27
申请号：US17509229
申请日：2021-10-25
摘要： A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.
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公开(公告)号：US20220195364A1
公开(公告)日：2022-06-23
申请号：US17689745
申请日：2022-03-08
摘要： The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.
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