Patent search ap:("President AND Fellows of Harvard College") AND inv:"Daniel Levner" Page 2

11.

发明申请
Compositions and Methods for Analyte Detection 有权

公开(公告)号：US20210262011A1

公开(公告)日：2021-08-26

申请号：US17238642

申请日：2021-04-23

Applicant: President and Fellows of Harvard College

Inventor： George M. Church , Jehyuk Lee , Daniel Levner , Michael Super

IPC: C12Q1/6806 , C12Q1/6874 , C12Q1/6869 , C12Q1/6844 , C12P19/34

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

12.

发明申请
Compositions and Methods for Analyte Detection 有权

公开(公告)号：US20210254131A1

公开(公告)日：2021-08-19

申请号：US17238682

申请日：2021-04-23

Applicant: President and Fellows of Harvard College

Inventor： George M. Church , Jehyuk Lee , Daniel Levner , Michael Super

IPC: C12Q1/6806 , C12Q1/6874 , C12Q1/6869 , C12Q1/6844 , C12P19/34

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

13.

发明授权
Compositions and methods for analyte detection 有权

公开(公告)号：US11021737B2

公开(公告)日：2021-06-01

申请号：US16941585

申请日：2020-07-29

Applicant: President and Fellows of Harvard College

Inventor： George M. Church , Je-hyuk Lee , Daniel Levner , Michael Super

IPC: C12Q1/68 , C12P19/34 , C12Q1/6806 , C12Q1/6874 , C12Q1/6869 , C12Q1/6844

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

14.

发明申请
SYSTEMS AND METHODS FOR CELL CULTURE DEVICE INTERCONNECTION AND FLUIDIC DEVICE INTERCONNECTION 审中-公开

公开(公告)号：US20200277558A1

公开(公告)日：2020-09-03

申请号：US16593646

申请日：2019-10-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber , Daniel Levner , Guy Thompson, II , Jose Fernandez-Alcon , Christopher David Hinojosa

IPC: C12M1/00 , C12M3/06 , B01L3/00 , C12M3/00 , C12M1/12 , C12M1/36

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

15.

发明申请
Compositions and Methods for Analyte Detection 审中-公开

公开(公告)号：US20190241938A1

公开(公告)日：2019-08-08

申请号：US16255920

申请日：2019-01-24

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner , Je-Hyuk Lee , George M. Church , Michael Super

IPC: C12Q1/6837 , C12Q1/6816 , C12Q1/6804

CPC classification number: C12Q1/6837 , C12Q1/6804 , C12Q1/6816 , G01N33/53 , G01N2458/10 , C12Q2535/122 , C12Q2537/143 , C12Q2563/107 , C12Q2563/179 , C12Q2565/514

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

16.

发明授权
Removing bubbles in microfluidic systems 有权

公开(公告)号：US09962698B2

公开(公告)日：2018-05-08

申请号：US14424195

申请日：2013-09-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber , Geraldine A. Hamilton , Daniel Levner , Christopher Hinojosa , Daniel Patterson

IPC: B01D19/00 , B01L3/00 , C12M1/107 , C12M1/04 , C12M3/06 , B01D63/08 , B01D71/36 , B01D17/00

CPC classification number: B01L3/502723 , B01D17/085 , B01D19/0031 , B01D63/088 , B01D71/36 , B01D2325/38 , B01L3/502715 , B01L2200/0684 , B01L2300/06 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0877 , B01L2300/0887 , C12M23/16 , C12M23/24 , C12M23/36

Abstract: A microfluidic system includes a microfluidic device connected to a bubble trap device whereby fluid flowing to the microfluidic device passes through the bubble trap device to remove gas bubbles prior to entering the microfluidic device. The bubble trap can include a separation chamber and an exhaust chamber separated by a hydrophobic porous membrane and gas bubbles in the fluid entering the separation chamber pass through the hydrophobic porous membrane into the exhaust chamber while the fluid remains in the separation chamber. The bubble trap can be formed by bonding a first body portion to a first side of the hydrophobic porous membrane and bonding a second body portion to a second side of the hydrophobic porous membrane. The exhaust chamber can be connected to an elongated exhaust channel that limits the evaporation losses of the fluid through the hydrophobic porous membrane.

17.

发明申请
Microfluidic Device Having Offset, High-Shear Seeding Channels 审中-公开

公开(公告)号：US20180119081A1

公开(公告)日：2018-05-03

申请号：US15566566

申请日：2016-04-08

Applicant: President and Fellows of Harvard College

Inventor： Karel Domansky , Christopher David Hinojosa , Donald E. Ingber , Daniel Levner , Guy Thompson, II

IPC: C12M3/06 , C12M1/00 , C12M1/12

CPC classification number: C12M23/16 , B01L3/5027 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , C12M25/02 , C12M29/14 , C12M35/04 , C12M35/08 , C12M41/00

Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.

18.

发明授权
Rapid antibiotic susceptibility testing 有权

公开(公告)号：US09632085B2

公开(公告)日：2017-04-25

申请号：US14382043

申请日：2013-02-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super , Donald E. Ingber , Mark J. Cartwright , Alexander Watters , John Samuel Workman , Daniel Levner , Martin Marcus Rottman

IPC: G01N33/53 , G01N33/569 , C12Q1/18

CPC classification number: G01N33/56938 , C12Q1/18 , G01N33/56911 , G01N33/56916 , G01N2333/245 , G01N2333/31 , G01N2500/10

Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.

19.

发明申请
Compositions and Methods for Analyte Detection 有权

公开(公告)号：US20250129408A1

公开(公告)日：2025-04-24

申请号：US18989169

申请日：2024-12-20

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner , Je-Hyuk Lee , George M. Church , Michael Super

IPC: C12Q1/6806 , C12P19/34 , C12Q1/6804 , C12Q1/6816 , C12Q1/6837 , C12Q1/6844 , C12Q1/6869 , C12Q1/6874 , G01N33/53

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.