公开(公告)号：US06283730B1

公开(公告)日：2001-09-04

申请号：US09441230

申请日：1999-11-16

申请人： Yasuhiko Sasaki ,  Yasuhiro Yoshimura ,  Akira Koide ,  Ryo Miyake ,  Naruo Watanabe ,  Takao Terayama ,  Hiroshi Mitsumaki ,  Yasuhiko Ishida ,  Tomonari Morioka

发明人： Yasuhiko Sasaki ,  Yasuhiro Yoshimura ,  Akira Koide ,  Ryo Miyake ,  Naruo Watanabe ,  Takao Terayama ,  Hiroshi Mitsumaki ,  Yasuhiko Ishida ,  Tomonari Morioka

IPC分类号： F04B1700

CPC分类号： F04B43/043

摘要： A micro pump is made compact and can prevent members constituting the micro pump from being chemically reacted with a working fluid, and a method of producing the same. After each of substrates constituting the micro pump is formed by a member containing a silicone as a main composition and a plurality of metal membranes are formed on a whole of a bonding surface of each of the substrates so as to form bonding surfaces, the bonding surfaces are cleaned, and the bonding surfaces are opposed to each other under a vacuum condition, overlapped, heated and pressed so as to be bonded. The valve portion has a beam and a protrusion for sealing as provided in the valve side, whereby a pressure applied to the protrusion becomes smaller than the bonding pressure.

摘要翻译： 微型泵制造紧凑，并且可以防止构成微型泵的构件与工作流体发生化学反应，及其制造方法。 在构成微型泵的基板的每一个由含有硅酮作为主要成分的构件形成，并且在每个基板的整个接合表面上形成多个金属膜以形成接合表面， 被清洁，并且粘合表面在真空条件下彼此相对，重叠，加热和压制以便粘合。 阀部具有设置在阀侧的梁和用于密封的突起，由此施加到突起的压力变得小于接合压力。

公开(公告)号：US06398930B2

公开(公告)日：2002-06-04

申请号：US09828946

申请日：2001-04-10

申请人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

发明人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

IPC分类号： G01N2700

CPC分类号： C02F1/008 ,  C02F2103/02 ,  C02F2201/008 ,  C02F2201/009 ,  C02F2209/11 ,  C02F2209/29 ,  C02F2209/40 ,  G01N33/1893 ,  Y02A20/212

摘要： A water quality meter is composed of a plurality of analyzing units for analyzing water samples introduced from a water distribution pipe, each analyzing unit including a reagent mixing cell and a measuring cell, and a liquid introducing unit integrated with the analyzing units, which is composed of a single member in which a plurality of fluid flows paths for feeding various types of liquid including the water sample into the analyzing unit are formed. Furthermore, the cells and the plurality of three-dimensional fluid flow paths formed in the single member are fabricated by a micro-fabrication technique using photo-curing resin. In one embodiment, a photometer is situated in the flow path in at least one analyzing unit for measuring absorbency of the liquid. In another embodiment, a pair of electrodes, for measuring electric conductivity or pH, is disposed in a measurement flow path in at least one of the analyzing units.

摘要翻译： 水质计由多个用于分析从配水管引入的水样的分析单元构成，每个分析单元包括试剂混合室和测量池，以及与分析单元一体化的液体导入单元，其组成 其中形成有多个流体流动路径的单个构件，用于将包括水样本的各种类型的液体进料到分析单元中。 此外，通过使用光固化树脂的微制造技术来制造在单个构件中形成的电池和多个三维流体流动路径。 在一个实施例中，光度计位于至少一个分析单元中的流路中，用于测量液体的吸收性。 在另一个实施例中，用于测量电导率或pH的一对电极设置在至少一个分析单元中的测量流路中。

公开(公告)号：US5174162A

公开(公告)日：1992-12-29

申请号：US550543

申请日：1990-07-10

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

IPC分类号： B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01L3/02 ,  G01N1/00 ,  G01N21/78 ,  G01N33/543 ,  G01N35/02 ,  G01N35/10

CPC分类号： B01F11/0071 ,  B01F11/0074 ,  B01F13/00 ,  B01F5/0609 ,  B01L3/021 ,  G01N35/10 ,  G01N2035/1058

摘要： A pipetter capable of mixing liquids and dispensing the mixed liquid has a pipetting tube, an actuating device connected to the tube for sucking liquid into and discharging liquid from the tube, and a flow disturbance device in the tube spaced from the discharge and suction end thereof and adapted to cause longitudinal mixing of liquid in the tube when liquid is moved along the tube in at least one direction. Mixing is effected in the tube by causing a liquid body to move repeatedly to and fro along the tube so that during each such movement at least a portion of the liquid body passes the flow disturbance device and the liquid body is not discharged from the first end of the tube. The pipetter is useful for example in mixing a suspension of blood cells with a reagent prior to analysis.

摘要翻译： 能够混合液体并分配混合液体的移液器具有移液管，连接到管的致动装置，用于将液体从管吸入和排出液体，以及与排出和吸入端隔开的管中的流动扰动装置 并且适于当液体沿着至少一个方向移动时引起液体在管中的纵向混合。 通过使液体沿着管道来回重复地在管中进行混合，使得在每次这样的移动期间，液体的至少一部分通过流动扰动装置，液体不从第一端排出 的管。 移液器可用于例如在分析之前将血细胞悬浮液与试剂混合。

公开(公告)号：US06444172B1

公开(公告)日：2002-09-03

申请号：US09828902

申请日：2001-04-10

申请人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

发明人： Masao Fukunaga ,  Tamio Ishihara ,  Koji Saito ,  Katsutoshi Yamada ,  Hideo Enoki ,  Sadao Mori ,  Ryo Miyake ,  Takao Terayama ,  Masatoshi Kanamaru

IPC分类号： G01N1500

摘要： A water quality meter is composed of a plurality of water quality monitoring meters including analyzing units for analyzing water samples introduced from a water distribution pipe, each analyzing unit analyzing the water samples and a measuring cell, and a liquid introducing unit integrated with the analyzing units, which is composed of a single member in which a plurality of fluid flow paths for feeding various types of liquid including the water sample into the analyzing unit are formed. Furthermore, the cells and the plurality of three-dimensional fluid flow paths formed in the single member are fabricated by a micro-fabrication technique using photo-curing resin.

公开(公告)号：US5079959A

公开(公告)日：1992-01-14

申请号：US404559

申请日：1989-09-08

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki ,  Toshio Kaneko ,  Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Kaori Yasuda

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki ,  Toshio Kaneko ,  Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Kaori Yasuda

IPC分类号： G01N15/12 ,  G01N15/14 ,  G01N35/10

CPC分类号： G01N35/1083 ,  G01N15/1404 ,  G01N2035/1086 ,  G01N35/1004

摘要： Particles contained in a liquid sample are analyzed by moving the particles along a detection passage past a detector using a liquid flow in the detection passage. To reduce the time required for analysis of each sample, the sample is delivered at an input location at the upstream end of the detection passage by movement of a movable container carrying the sample to the input location. Immediately after the commencement of the delivery of the liquid sample, liquid flow is caused to occur in the detection passage. Washing liquid is supplied to the input location by a liquid supplier different from the movable container which carries the sample.

公开(公告)号：US5690895A

公开(公告)日：1997-11-25

申请号：US761143

申请日：1996-12-06

申请人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

发明人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

IPC分类号： G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N21/05 ,  G01N33/49

CPC分类号： G01N21/05 ,  G01N15/1404 ,  G01N2021/0346

摘要： A flow cell apparatus, useful in an apparatus for measuring particles suspended in a liquid, comprises a sample liquid, supply device, a sheath liquid supply device, a flow cell which has a flow passage passing through a transparent measuring portion, and a nozzle for causing the sample liquid, from the supply device to flow in the flow passage of the flow cell. The nozzle has at least one discharge port which is disposed in the flow passage in a spaced relation from the inner wall of the flow cell flow passage. The sheath liquid supply device causes the sheath liquid to flow around the nozzle discharge port and surround the sample liquid flow to form a sheath flow. The flow passage of the flow cell and the nozzle are so formed as to ensure a sample liquid flow of a fixed width through the measuring portion. A stable sample flow having a fixed width can be formed in the measuring portion even when the flow is at a high velocity, and particles in the sample liquid can efficiently be photographed with high accuracy.

摘要翻译： 一种用于测量悬浮在液体中的颗粒的装置中的流动池装置，包括样品液体，供给装置，皮肤液体供应装置，具有通过透明测量部分的流动通道的流动池，以及用于 使来自供给装置的样品液体流入流动池的流路。 喷嘴具有至少一个排出口，该排出口以与流动池流动通道的内壁隔开的关系设置在流动通道中。 皮肤液体供给装置使皮肤液体在喷嘴排出口周围流动并且围绕样品液体流动形成鞘流。 流动池和喷嘴的流动通道形成为确保通过测量部分具有固定宽度的样品液体流动。 即使在流速高的情况下，也可以在测量部分形成具有固定宽度的稳定的样品流，并且可以高精度地有效地拍摄样品液体中的颗粒。

公开(公告)号：US4863264A

公开(公告)日：1989-09-05

申请号：US203457

申请日：1988-06-07

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki

IPC分类号： G01N15/14 ,  G01N33/48

CPC分类号： G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1452

摘要： A photoanalysis apparatus includes marker pouring device for pouring markers into one of sheath fluid and fluid carrying particles in a flow-cell device, second optical device for applying a light beam for correction to the markers in the flow-cell device to measure strength of fluorescence or light scattering caused by said markers to generate a signal for correction, second signal processing device for calculating from the signal for correction at least one of a difference in position between a center of the fluid carrying particles in the flow-cell device and a center of a light beam for examination and a difference in flow rate between a predetermined flow rate and an actual flow rate of the fluid carrying particles in the flow-cell device to generate correspondingly at least one of a first command signal for position correction and a second command signal for flow rate correction, and correspondingly at least one of first correcting device for shifting the center of the light beam for examination to the center of the flow of the fluid carrying particles in accordance with the first command signal and second correcting device for correcting the flow rate of the fluid carrying particles to the predetermined flow rate in accordance with the second command signal.

公开(公告)号：US5270212A

公开(公告)日：1993-12-14

申请号：US841487

申请日：1992-02-26

申请人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

发明人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N15/00 ,  G01N15/14 ,  G01N33/48 ,  G01N35/00

CPC分类号： G01N35/1095 ,  G01N1/28 ,  G01N2015/0069 ,  G01N2015/0076 ,  G01N2015/1409 ,  G01N2021/6439 ,  Y10T436/111666

摘要： This invention relates to an apparatus for analyzing cells in a biological liquid sample, such as blood, in which apparatus a flow passage in a sample pretreatment system can be shortened with a simple construction. A sample metering chamber, a mixing chamber and a measurement liquid extracting chamber are formed in a rotary sliding member of a pretreatment portion. The rotary sliding member is intermittently rotated so as to shift a sample from one chamber to another to perform a necessary treatment. Fluorescence or scattering light with respect to the sample, mixed with a reagent in the pretreatment portion, is detected by a flow cell.

摘要翻译： 本发明涉及用于分析生物液体样品如血液中的细胞的装置，其中可以用简单的结构缩短样品预处理系统中的流动通道。 样品计量室，混合室和测量液体提取室形成在预处理部分的旋转滑动构件中。 旋转滑动构件间歇地旋转，以将样品从一个室移动到另一个室进行必要的处理。 通过流动池检测相对于样品的荧光或散射光与预处理部分中的试剂混合。

公开(公告)号：US5880835A

公开(公告)日：1999-03-09

申请号：US703076

申请日：1996-08-26

申请人： Isao Yamazaki ,  Hiroshi Ohki ,  Masaetsu Matsumoto ,  Ryo Miyake ,  Ryohei Yabe ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

发明人： Isao Yamazaki ,  Hiroshi Ohki ,  Masaetsu Matsumoto ,  Ryo Miyake ,  Ryohei Yabe ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

IPC分类号： G01N15/14 ,  G01N15/02 ,  G01N15/06 ,  G01N33/48 ,  G01N33/49 ,  G01N33/493

CPC分类号： G01N15/147 ,  G01N2015/1409 ,  G01N33/49 ,  G01N33/493

摘要： In order to investigate particles in a fluid, a flow cell causes the fluid to flow past a sensor which receives light from a continuous light emission system. When the sensor detects a change in light, a particle detector is triggered, which in turn triggers a pulse generator and hence an intermittent light emission system. Light then illuminates the flow cell to allow a CCD camera to photograph the particle. The photographs taken by the CCD camera are analyzed by an image processor. In order to obtain an accurate particle concentration measurement, it is necessary to modify the initial concentration measurement derived from the analysis of particle images by the image processor by a compensation coefficient. This operation is carried out by a concentration compensator. The result may then be displayed. Additional analysis may be carried out by changing the magnification of the CCD camera, or by selecting for analysis only those particles of a selected size or type.

摘要翻译： 为了研究流体中的颗粒，流动池使得流体流过从连续发光系统接收光的传感器。 当传感器检测到光的变化时，触发粒子检测器，这又触发脉冲发生器，因此触发间歇发光系统。 光然后照亮流动池，以允许CCD照相机拍摄颗粒。 由CCD照相机拍摄的照片由图像处理器分析。 为了获得准确的粒子浓度测量，需要通过图像处理器修正由粒子图像的分析得到的初始浓度测量值的补偿系数。 该操作由浓度补偿器进行。 然后可以显示结果。 可以通过改变CCD照相机的放大倍率或通过选择仅分析选定尺寸或类型的那些颗粒来进行附加分析。

公开(公告)号：US4983038A

公开(公告)日：1991-01-08

申请号：US178651

申请日：1988-04-07

申请人： Hiroshi Ohki ,  Ryo Miyake ,  Isao Yamazaki ,  Fujiya Takahata

发明人： Hiroshi Ohki ,  Ryo Miyake ,  Isao Yamazaki ,  Fujiya Takahata

IPC分类号： B01F13/00 ,  G01N15/14

CPC分类号： G01N15/1404 ,  B01F13/0062

摘要： A sheath flow type flow-cell device for photo-particle-analyzer comprises a substantially flat top surface, at least one first inlet for sheath fluid, at least one first flow passage communicated with the at least one first inlet and contracted toward downstream to be a straight capillary flow passage, a discharge port provided at a terminal end of the straight capillary flow passage, a second inlet for sample fluid, a second flow passage for sample fluid communicated with the second inlet and contracted toward downstream, and coupling portions for sealingly coupling with the particle photo-analyzer. The second flow passage is opened within the at least one first flow passage so as to keep a part of the at least one first flow passage above and below an opening of the second flow passage. The opening is faced in the same direction as the straight capillary flow passage. A wall portion on which the opening locates is rounded. The wall portion is further chamfered and a pair of projections are extended from the wall portion.