公开(公告)号：US10669304B2

公开(公告)日：2020-06-02

申请号：US15015059

申请日：2016-02-03

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Pierre F. Indermuhle ,  Eugene P. Marsh ,  Andres Fernandez ,  William Banyai ,  Bill J. Peck

IPC: C07H21/04 ,  B01J19/00 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455

Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.

公开(公告)号：US10583415B2

公开(公告)日：2020-03-10

申请号：US16409608

申请日：2019-05-10

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C12N15/66 ,  C12N15/74 ,  C40B50/00 ,  C40B40/06 ,  C40B50/14 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20150038373A1

公开(公告)日：2015-02-05

申请号：US14452429

申请日：2014-08-05

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: C12N15/10 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Abstract translation: 本文提供了从头合成的大型核酸文库，其错误率低。 此外，本文描述了用于制造高质量构建块（例如寡核苷酸）的装置。 可以使用微流控组件并行合成更长的核酸。 此外，本文的方法允许快速构建长，高质量基因的大型文库。 用于制造长质量和高质量核酸的大型文库的装置在本文中进一步描述。

公开(公告)号：US11697668B2

公开(公告)日：2023-07-11

申请号：US16854719

申请日：2020-04-21

Applicant: Twist Bioscience Corporation

Inventor： Pierre F. Indermuhle ,  Eugene P. Marsh ,  Andres Fernandez ,  William Banyai ,  Bill J. Peck

IPC: C07H21/04 ,  B01J19/00 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455

CPC classification number: C07H21/04 ,  B01J19/0046 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455 ,  B01J2219/0059 ,  B01J2219/00527 ,  B01J2219/00596

Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.

公开(公告)号：US11559778B2

公开(公告)日：2023-01-24

申请号：US16039256

申请日：2018-07-18

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US11185837B2

公开(公告)日：2021-11-30

申请号：US16535777

申请日：2019-08-08

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B40/06 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US10384189B2

公开(公告)日：2019-08-20

申请号：US15860445

申请日：2018-01-02

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Bill James Peck ,  Pierre Indermuhle ,  Eugene P. Marsh ,  Andres Fernandez ,  David Stern

IPC: B01J19/00

Abstract: Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.

公开(公告)号：US09981239B2

公开(公告)日：2018-05-29

申请号：US15135434

申请日：2016-04-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle ,  Eugene P. Marsh

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01L3/50857 ,  C12N15/1093 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

公开(公告)号：US11452980B2

公开(公告)日：2022-09-27

申请号：US16737401

申请日：2020-01-08

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: C12N15/10 ,  C40B50/18 ,  C12N15/66 ,  C12N15/74 ,  C40B50/00 ,  C40B40/06 ,  C40B50/14 ,  C12N15/09 ,  B01J19/00

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20200299322A1

公开(公告)日：2020-09-24

申请号：US16854719

申请日：2020-04-21

Applicant: Twist Bioscience Corporation

Inventor： Pierre F. Indermuhle ,  Eugene P. Marsh ,  Andres Fernandez ,  William Banyai ,  Bill J. Peck

IPC: C07H21/04 ,  B01J19/00 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455

Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.