公开(公告)号：US08685328B2

公开(公告)日：2014-04-01

申请号：US13060700

申请日：2009-05-21

申请人： Hiroaki Okano ,  Kazuo Nishikawa ,  Hisaharu Yagi ,  Norihiro Matsuoka ,  Tomonori Akai ,  Misaki Nakamura ,  Yoshihiro Shimizu

发明人： Hiroaki Okano ,  Kazuo Nishikawa ,  Hisaharu Yagi ,  Norihiro Matsuoka ,  Tomonori Akai ,  Misaki Nakamura ,  Yoshihiro Shimizu

IPC分类号： A62B7/08 ,  A61L2/00 ,  A61L9/00 ,  C25B5/00 ,  H05F3/00

CPC分类号： A61L9/22 ,  A61L2202/14 ,  A61L2202/25 ,  F24F3/166 ,  F24F2001/0096 ,  F24F2003/1682

摘要： In order to remove a pathogenic effect of a microorganism in a room or a work space in a short period of time, an ion diffusing apparatus, which includes an ion generator (17, 18) for generating positive ions each including H+(H2O)m and negative ions each including O2−(H2O)n, where m and n are arbitrary integers, and a blower for delivering the positive ions and the negative ions, which are generated from the ion generator (17), from a blowout opening, is operated to widely distribute, with a high concentration, the positive ions and the negative ions in the room.

摘要翻译： 为了在短时间内除去微生物在室内或工作空间中的致病作用，可以使用离子扩散装置，该离子扩散装置包括用于产生正离子的离子发生器（17,18），每个离子发生器包括H +（H 2 O）m 和负离子各自包括O 2 - （H 2 O）n，其中m和n是任意整数，并且用于从吹出口从离子发生器（17）输出正离子和负离子的鼓风机是 以高浓度广泛分布室内的正离子和负离子。

公开(公告)号：US20130244328A1

公开(公告)日：2013-09-19

申请号：US13990642

申请日：2010-12-06

申请人： Naoki Yokoyama ,  Tomonori Akai

发明人： Naoki Yokoyama ,  Tomonori Akai

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12M25/08 ,  C12M33/00 ,  C12N2529/00 ,  C12N2539/10

摘要： This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated.

摘要翻译： 本发明提供了在特定条件下修饰细胞培养基质的表面特性的方法，根据细胞种类，调节允许细胞粘附或不允许粘附的区域。 本发明涉及一种细胞培养方法，其包括以下步骤：借助于硅烷将正电位施加到包括具有导电区域的基底材料和与其偶联的非细胞粘附膜的基底的导电区域，以便 以将非细胞粘附膜与基底分离; 并在非细胞粘附膜分离的区域中培养细胞。

公开(公告)号：US20130116499A1

公开(公告)日：2013-05-09

申请号：US13807346

申请日：2011-06-30

申请人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

发明人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

IPC分类号： C12Q1/04 ,  A61B17/435

CPC分类号： G06T7/0016 ,  A01K67/027 ,  A01K2227/101 ,  A61B17/435 ,  C12M21/06 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0604 ,  C12Q1/04 ,  G01N33/4833 ,  G06T2207/10016 ,  G06T2207/30044

摘要： An object of the present invention is to provide a means for efficiently obtaining mammalian embryos having high conception rates. A first aspect of the present invention is a method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg, comprising a step of selecting an embryo using two or more of the following indicators: the time from fertilization to the completion of first cleavage; the morphology at a stage after first cleavage and before second cleavage; the morphology at a stage after third cleavage and before fourth cleavage; and the amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage.

摘要翻译： 本发明的目的是提供一种有效地获得具有高受孕率的哺乳动物胚胎的方法。 本发明的第一方面是一种选择通过从受精卵进行体外培养而制备的哺乳动物胚胎的方法，其包括使用以下两个或更多个指标来选择胚胎的步骤：从受精到完成第一次的时间 裂解 在第一次切割后和第二次切割之前的阶段的形态; 在第三次切割后和第四次切割之前的阶段的形态; 以及早期胚泡期，胚泡期或扩大胚泡期消耗的氧量。

公开(公告)号：US20130075793A1

公开(公告)日：2013-03-28

申请号：US13701610

申请日：2011-06-14

申请人： Takuya Higuchi ,  Tomonori Akai ,  Katsuyuki Motai

发明人： Takuya Higuchi ,  Tomonori Akai ,  Katsuyuki Motai

IPC分类号： H01L29/78

CPC分类号： H01L29/78 ,  G01N27/4145

摘要： Provided is a biosensor that makes it possible to detect the electrical properties of a bio-related material contained in an analyte fluid such as an aqueous solution placed on a sensitive membrane and to observe the bio-related material at a high magnification with an observation device such as a microscope. The biosensor comprises: a substrate 10, a transparent semiconductor film 20 laminated on the substrate 10, a source electrode 30a and a drain electrode 30b formed at both ends of the transparent semiconductor film 20, an insulating film 40 laminated so as to cover the transparent semiconductor film 20 and the source electrode 30a and the drain electrodes 30b, a sensitive membrane 50 laminated on the insulating film 40, and a partition 60 that is formed at both ends of the sensitive membrane 50 to retain an analyte fluid 100, such as an aqueous solution or liquid culture containing a sample, on the sensitive membrane 50 and to retain the analyte fluid 100 in a specific region so that the bio-related material 200 can be placed on the sensitive membrane 50.

摘要翻译： 提供了一种生物传感器，其可以检测包含在诸如放置在敏感膜上的水溶液的分析物流体中的生物相关材料的电性能，并且用观察装置高倍率观察生物相关材料 如显微镜。 生物传感器包括：基板10，层叠在基板10上的透明半导体膜20，形成在透明半导体膜20的两端的源电极30a和漏极30b，层叠以覆盖透明半导体膜20的绝缘膜40 半导体膜20以及源电极30a和漏电极30b，层压在绝缘膜40上的敏感膜50以及形成在敏感膜50的两端以保持分析物流体100的分隔件60，例如 含有样品的水溶液或液体培养物在敏感膜50上并将分析物流体100保持在特定区域中，使得生物相关材料200可以放置在敏感膜50上。

公开(公告)号：US06805979B2

公开(公告)日：2004-10-19

申请号：US10136518

申请日：2002-05-02

申请人： Takashi Ogura ,  Shinji Yamana ,  Tomonori Akai

发明人： Takashi Ogura ,  Shinji Yamana ,  Tomonori Akai

IPC分类号： B32B702

CPC分类号： H01L51/0013 ,  H01L27/32 ,  H01L27/3244 ,  H01L51/0004 ,  H01L51/0059 ,  H01L51/0081 ,  Y10S428/917 ,  Y10T428/24942

摘要： A transfer film comprising a base film, a transfer layer, and a transfer auxiliary layer formed between the base film and the transfer layer so as to be in contact with at least the transfer layer, this transfer auxiliary layer having a melting point or a glass transition temperature lower than that of the transfer layer, wherein at least a portion of the transfer layer can be thermally transferred onto a substrate.

摘要翻译： 一种转印膜，包括基底膜，转印层和形成在基膜和转印层之间的转印辅助层，以便与至少转印层接触，该转印辅助层具有熔点或玻璃 转移温度低于转移层的转变温度，其中转移层的至少一部分可以热转印到基底上。

公开(公告)号：US09155159B2

公开(公告)日：2015-10-06

申请号：US12680046

申请日：2008-09-29

申请人： Masato Okada ,  Shigehiro Ueno ,  Tomonori Akai ,  Masaya Shimogawara ,  Seiji Take

发明人： Masato Okada ,  Shigehiro Ueno ,  Tomonori Akai ,  Masaya Shimogawara ,  Seiji Take

IPC分类号： H01L33/04 ,  H01L51/54 ,  H05B33/14 ,  H01L51/50 ,  H01L51/00

CPC分类号： H05B33/145 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/5012 ,  H01L51/502

摘要： A QD protecting material having high compatibility with a binder component in a luminescent layer. The luminescent layer contains, as a part of its chemical structure, a compound containing a moiety A having a sum atomic weight MA of 100 or more and quantum dots protected by a protecting material, the protecting material contains, as a part of its chemical structure, a linking group connected to a quantum dot surface and a moiety B that has a sum atomic weight MB of 100 or more, satisfies a relationship between MB and MA represented by |MA−MB|/MB (2, and satisfies the requirement that the sum atomic weight MB is larger than one-third of the molecular weight of the protecting material, and a solubility parameter SA of the moiety A and a solubility parameter SB of the moiety B satisfy a relationship represented by |SA (SB| (2.

摘要翻译： 与发光层中的粘合剂成分高度相容的QD保护材料。 作为其化学结构的一部分，发光层含有总原子量为100以上的部分A和保护材料保护的量子点的化合物，保护材料作为其化学结构的一部分 连接到量子点表面的连接基团和总原子量MB为100以上的部分B满足由MA-MB | / MB（2）表示的MB和MA之间的关系，并且满足以下要求： 总原子量MB大于保护材料分子量的三分之一，部分A的溶解度参数SA和部分B的溶解度参数SB满足由| SA（SB |（2） 。

公开(公告)号：US09062341B2

公开(公告)日：2015-06-23

申请号：US13878726

申请日：2010-12-06

申请人： Naoki Yokoyama ,  Tomonori Akai ,  Yuya Takasugi

发明人： Naoki Yokoyama ,  Tomonori Akai ,  Yuya Takasugi

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  C12Q1/045

摘要： This invention provides a means for cell migration assays by regulating the cells adhered to a substrate so that they migrate to a given region, which is a substrate used for cell culture comprising: a base material comprising a conductive region and an insulating region provided thereon and cell-adhesive regions and non-cell-adhesive regions provided in the conductive region and the insulating region, respectively, wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region, and a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the insulating region.

摘要翻译： 本发明提供了一种用于细胞迁移测定的方法，其通过调节附着于基底的细胞使得它们迁移到给定区域，所述给定区域是用于细胞培养的底物，其包含：基材，其包含导电区域和设置在其上的绝缘区域， 设置在导电区域和绝缘区域中的细胞粘附区域和非细胞粘合剂区域，其中细胞粘附区域邻近导电区域中的非细胞粘附区域定位，并且细胞粘合剂 区域与绝缘区域中的非细胞粘合剂区域相邻。

公开(公告)号：US08759100B2

公开(公告)日：2014-06-24

申请号：US13990642

申请日：2010-12-06

申请人： Naoki Yokoyama ,  Tomonori Akai

发明人： Naoki Yokoyama ,  Tomonori Akai

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12M25/08 ,  C12M33/00 ,  C12N2529/00 ,  C12N2539/10

摘要： This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated.

摘要翻译： 本发明提供了在特定条件下修饰细胞培养基质的表面特性的方法，根据细胞种类，调节允许细胞粘附或不允许粘附的区域。 本发明涉及一种细胞培养方法，其包括以下步骤：借助于硅烷将正电位施加到包括具有导电区域的基底材料和与其偶联的非细胞粘附膜的基底的导电区域，以便 以将非细胞粘附膜与基底分离; 并在非细胞粘附膜分离的区域中培养细胞。

公开(公告)号：US20100237322A1

公开(公告)日：2010-09-23

申请号：US12680046

申请日：2008-09-29

申请人： Masato Okada ,  Shigehiro Ueno ,  Tomonori Akai ,  Masaya Shimogawara ,  Seiji Take

发明人： Masato Okada ,  Shigehiro Ueno ,  Tomonori Akai ,  Masaya Shimogawara ,  Seiji Take

IPC分类号： H01L33/04

CPC分类号： H05B33/145 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/5012 ,  H01L51/502

摘要： A QD protecting material having high compatibility with a binder component in a luminescent layer. The luminescent layer contains, as a part of its chemical structure, a compound containing a moiety A having a sum atomic weight MA of 100 or more and quantum dots protected by a protecting material, the protecting material contains, as a part of its chemical structure, a linking group connected to a quantum dot surface and a moiety B that has a sum atomic weight MB of 100 or more, satisfies a relationship between MB and MA represented by |MA−MB|/MB (2, and satisfies the requirement that the sum atomic weight MB is larger than one-third of the molecular weight of the protecting material, and a solubility parameter SA of the moiety A and a solubility parameter SB of the moiety B satisfy a relationship represented by |SA (SB| (2.

摘要翻译： 与发光层中的粘合剂成分高度相容的QD保护材料。 作为其化学结构的一部分，发光层含有总原子量为100以上的部分A和保护材料保护的量子点的化合物，保护材料作为其化学结构的一部分 连接到量子点表面的连接基团和总原子量MB为100以上的部分B满足由MA-MB | / MB（2）表示的MB和MA之间的关系，并且满足以下要求： 总原子量MB大于保护材料分子量的三分之一，部分A的溶解度参数SA和部分B的溶解度参数SB满足由| SA（SB |（2） 。

公开(公告)号：US20100221768A1

公开(公告)日：2010-09-02

申请号：US12702526

申请日：2010-02-09

申请人： Tomonori Akai ,  Yasuhito Onishi ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Tamas Somfai

发明人： Tomonori Akai ,  Yasuhito Onishi ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Tamas Somfai

IPC分类号： C12Q1/02 ,  C12M1/22

CPC分类号： C12M23/12 ,  C12M23/10

摘要： An object of the present invention is to provide a culture dish appropriate for automatically identifying cultured cells. The present invention relates to a culture dish for culturing cells that require separate control, which has a bottom wall and a side wall, wherein cell-holding parts having wells are arranged on the bottom wall, 4 or more wells are adjacent to each other, the wall surface of each well has a concave surface that slopes upward from the lowest position to the outer edge of the well, and the pitch between adjacent wells is 1 mm or less.

摘要翻译： 本发明的目的是提供一种适于自动识别培养细胞的培养皿。 本发明涉及一种用于培养需要分开控制的细胞的培养皿，其具有底壁和侧壁，其中具有孔的细胞保持部件布置在底壁上，4个或更多个孔彼此相邻， 每个井的壁表面具有从井的最低位置向外倾斜向上的凹面，相邻孔之间的间距为1mm以下。