Petal-array support for use with microplates
    11.
    发明申请
    Petal-array support for use with microplates 审中-公开
    花瓣阵列支持与微孔板一起使用

    公开(公告)号:US20050271553A1

    公开(公告)日:2005-12-08

    申请号:US11021039

    申请日:2004-12-21

    摘要: Devices are provided which include supports upon which one or more ion-exchange materials can be disposed for purifying a sample. In various embodiments, the supports include a plurality of deformable members, for example, petal-shaped purification members, that provide binding sites for ion-exchange material and optionally biochemical species, chemicals, salts, or other materials. An apparatus and method are also provided for the insertion and removal of the purification members into respective wells of a multi-well microplate.

    摘要翻译: 提供了包括可以设置一种或多种离子交换材料用于纯化样品的载体的装置。 在各种实施例中,支撑件包括多个可变形构件,例如花瓣形净化构件,其提供用于离子交换材料和任选的生化物质,化学物质,盐或其它材料的结合位点。 还提供了一种用于将净化构件插入和移除到多孔微板的相应孔中的装置和方法。

    Devices and Methods for Optoelectronic Manipulation of Small Particles
    13.
    发明申请
    Devices and Methods for Optoelectronic Manipulation of Small Particles 审中-公开
    小粒子光电子操作的装置和方法

    公开(公告)号:US20070095669A1

    公开(公告)日:2007-05-03

    申请号:US11552853

    申请日:2006-10-25

    IPC分类号: B03C5/02

    摘要: A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.

    摘要翻译: 用于分选生物样品中包含第一类型细胞和第二类型细胞的细胞的方法可以包括将生物样品引入包含第一表面和第二表面的室中,其中第一表面与透明电极相关联, 第二表面与电极的光电导部分相关联。 该方法还可以包括相对于彼此移动入射光和光电导部分,以照射光电导部分的区域并且调制在邻近照明区域的室中的电场。 该方法还可以包括通过由调制电场引起的第一类型的细胞和第二类型的细胞的介电电泳运动,将第一类型的细胞与腔室中的第二类型的细胞分离,其中至少一种的介电电泳特性 的第一类型的细胞和第二类型的细胞已被修改。

    Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
    15.
    发明申请
    Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis 审中-公开
    聚和共聚(N-乙烯基酰胺)及其在毛细管电泳中的应用

    公开(公告)号:US20050025741A1

    公开(公告)日:2005-02-03

    申请号:US10843114

    申请日:2004-05-11

    申请人: Aldrich Lau

    发明人: Aldrich Lau

    摘要: The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis.

    摘要翻译: 本发明一般涉及包含N-乙烯基酰胺型单体的聚合物和共聚物,它们的制备方法和组合物,例如含有它们的电泳分离介质; 包含这些聚合物的载体,如毛细管; 以及使用毛细管电泳分离生物分子,特别是多核苷酸的混合物的方法。 包含这种聚合物的分离介质通过毛细管电泳在生物分子的分析和分离中产生有利的性能。

    Surface Modification in a Manipulation Chamber
    16.
    发明申请
    Surface Modification in a Manipulation Chamber 审中-公开
    操纵室中的表面改性

    公开(公告)号:US20070095666A1

    公开(公告)日:2007-05-03

    申请号:US11423810

    申请日:2006-06-13

    申请人: Aldrich Lau Huan Phan

    发明人: Aldrich Lau Huan Phan

    IPC分类号: C07K1/26 G01N27/00

    CPC分类号: G01N27/305 B03C11/00 C07K1/26

    摘要: A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide)methyl ether)acrylate, a poly(2-hydroxyethyl(meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.

    摘要翻译: 一种用于操纵生物材料的装置,该装置包括至少一个电极和被配置为容纳生物材料的光导材料; 以及光源,被配置为照亮所述光电导材料以调制电场,其中所述电场被配置为操纵所述生物材料; 其中所述至少一个电极和/或光电导材料的表面用羧基部分,氨基部分,聚(乙二醇)部分,(聚(环氧乙烷)甲基醚)的聚合物, 丙烯酸酯,聚(甲基)丙烯酸2-羟乙酯),聚(N-乙烯基吡咯烷酮),聚(N-乙烯基甲酰胺),聚(N-乙烯基甲酰胺)衍生物,聚((甲基)丙烯酰胺) 聚((甲基)丙烯酰胺)衍生物。 还公开了用于操作生物材料的方法。

    Polymer monolith substrate
    19.
    发明申请
    Polymer monolith substrate 审中-公开
    聚合物整料基材

    公开(公告)号:US20060078983A1

    公开(公告)日:2006-04-13

    申请号:US11213635

    申请日:2005-08-25

    申请人: Aldrich Lau

    发明人: Aldrich Lau

    IPC分类号: C12M1/34 C08L43/00

    摘要: The present teachings provide for composite substrates for the covalent attachment of biomolecules and method of making the same. The present teachings provide for composite substrates comprising a porous copolymer-monolith covalently attached to a surface of a substrate, wherein the porous copolymer-monolith has been formed by an inverse phase photo-copolymerization process comprising photo-copolymerizing at least one ethylenically unsaturated monomer with polymerizable surface functionalities that are covalently attached to a surface of a derivitized substrate such that, after photo-copolymerization, the porous copolymer-monolith is covalently attached to the surface of the substrate, and wherein the photo-copolymerizing is carried out in the presence of at least one porogenic solvent.

    摘要翻译: 本发明提供用于共价连接生物分子的复合基底及其制备方法。 本教导提供了包含共价连接到基底表面的多孔共聚物单体的复合基底,其中多孔共聚物 - 整料通过反相光共聚方法形成,该反相光共聚方法包括使至少一种烯属不饱和单体与 共价连接到衍生基材的表面的可聚合表面官能团,使得在光共聚之后,多孔共聚物 - 整料共价连接到基材的表面,并且其中光共聚在 至少一种造孔溶剂。

    Size-exclusion ion-exchange particles
    20.
    发明申请
    Size-exclusion ion-exchange particles 审中-公开
    尺寸排阻离子交换颗粒

    公开(公告)号:US20060051583A1

    公开(公告)日:2006-03-09

    申请号:US11262036

    申请日:2005-10-27

    IPC分类号: B01D15/08 B32B5/16

    摘要: A size-exclusion ion-exchange particle and a device using the particle are provided wherein the particle includes an ion-exchange core micro-encapsulated by a shell, and the shell includes a polymerization product of a reactive monomer. The shell can be an at least partially cross-linked polymer. The shell can be capable of excluding molecules of a size equal to or larger than a 10 nt ssDNA molecule or molecules of a size equal to or larger than a 100 nt ssDNA molecule, for example, and the core can be capable of ion-exchange. Methods of making size-exclusion ion-exchange particles are also provided, as are methods of purification using the particles.

    摘要翻译: 提供了尺寸排阻离子交换颗粒和使用该颗粒的装置,其中颗粒包括由壳微囊封装的离子交换核,壳包括反应性单体的聚合产物。 壳可以是至少部分交联的聚合物。 壳可以排除等于或大于10nt ssDNA分子的分子或等于或大于100nt ssDNA分子的分子的分子,并且核可以能够进行离子交换 。 还提供了制备尺寸排阻离子交换颗粒的方法,以及使用该颗粒的纯化方法。