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38 条记录 (耗时 0.03 秒)

    Method for obtaining active pro-NGF and beta-NGF
    16.
    发明授权
    Method for obtaining active pro-NGF and beta-NGF 有权
    获得活性前NGF和β-NGF的方法

    公开(公告)号：US08501439B2

    公开(公告)日：2013-08-06

    申请号：US12765229

    申请日：2010-04-22

    申请人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    发明人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    IPC分类号： C12N15/18

    CPC分类号： C07K14/48

    摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.

    摘要翻译： 本发明涉及从前体proNGF生产生物活性β-NGF的方法。 在原核宿主细胞中表达β-NGF的形式后，以不溶性无活性聚集体（包涵体）的形式分离重组蛋白。 在将其溶解在强变性剂中并随后转化成通过存在于天然β-NGF中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性β-NGF。

    Pharmaceutical preparations comprising human proNGF
    17.
    发明授权
    Pharmaceutical preparations comprising human proNGF 有权
    包含人类proNGF的药物制剂

    公开(公告)号：US08318671B1

    公开(公告)日：2012-11-27

    申请号：US09807096

    申请日：1999-10-11

    申请人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    发明人： Anke Rattenholl ,  Adelbert Grossmann ,  Elisabeth Schwarz ,  Rainer Rudolph

    IPC分类号： C07K14/48 ,  A61K38/18

    CPC分类号： C07K14/48

    摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.

    摘要翻译： 本发明涉及从前体proNGF生产生物活性的NGF的方法。 在原核宿主细胞中表达生物素-NGF的形式后，重组蛋白以不溶性非活性聚集体（包涵体）的形式分离。 在将其溶解在强变性剂中并随后转化成通过存在于天然和/或生长因子中的二硫键确定的天然构象后，通过随后分离原序列获得生物活性 。

    Method for the synthesis and selective biocatalytic modification of peptides, peptide mimetics and proteins
    18.
    发明申请
    Method for the synthesis and selective biocatalytic modification of peptides, peptide mimetics and proteins 审中-公开
    肽，肽模拟物和蛋白质的合成和选择性生物催化修饰的方法

    公开(公告)号：US20060149035A1

    公开(公告)日：2006-07-06

    申请号：US10526163

    申请日：2003-09-01

    申请人： Rainer Rudolph ,  Frank Bordusa ,  Nicole Wehofsky

    发明人： Rainer Rudolph ,  Frank Bordusa ,  Nicole Wehofsky

    IPC分类号： C07K1/02

    CPC分类号： C07K1/12

    摘要： The present invention relates to a method for the synthesis of peptides, peptide mimetics and/or proteins and/or for the selective N-terminal modification of peptides, peptide mimetics and/or proteins, with the steps of: a) providing an amino component, said amino component having at least one amino acid, b) providing a carboxyl component, said carboxyl component having a leaving group on the carboxyl group, and said carboxyl component being a compound having at least one amino acid or a compound having at least one label or reporter group, c) reacting said amino component and said carboxyl component in a reaction medium which has one or more ionic liquids, in the presence of a protease, peptidase and/or hydrolase, to form a peptide bond between the amino component and the carboxyl component with elimination of the leaving group.

    摘要翻译： 本发明涉及用于合成肽，肽模拟物和/或蛋白质和/或肽，肽模拟物和/或蛋白质的选择性N-末端修饰的方法，其具有以下步骤：a）提供氨基成分 所述氨基组分具有至少一个氨基酸，b）提供羧基组分，所述羧基组分在羧基上具有离去基团，所述羧基组分是具有至少一个氨基酸的化合物或具有至少一个氨基酸的化合物 标记或报告基团，c）在蛋白酶，肽酶和/或水解酶的存在下，在具有一种或多种离子液体的反应介质中使所述氨基组分和所述羧基组分反应，以形成氨基组分和 羧基组分消除离去基团。