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公开(公告)号：US20210199624A1
公开(公告)日：2021-07-01
申请号：US17057361
申请日：2019-05-20
Applicant: Waters Technologies Corporation
Inventor： Martin Gilar , Thomas S. McDonald , Joseph D. Michienzi , Jeffrey Musacchio , Rachel Mumma , Keith Fadgen , Geoff C. Gerhardt
IPC: G01N30/06 , G01N1/40 , G01N30/54 , G01N30/60 , B01J20/24 , B01J20/28 , B01J20/288 , B01J20/32 , B01D15/38 , B01D15/22 , B01D15/16
Abstract: Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.
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公开(公告)号：US20210171932A1
公开(公告)日：2021-06-10
申请号：US17112502
申请日：2020-12-04
Applicant: Waters Technologies Corporation
Inventor： Michael Donegan , Martin Gilar , Matthew Lauber , Scott Mccall , Pamela Iraneta , Kerri Smith
IPC: C12N15/10 , C12N15/113
Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.
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公开(公告)号：US20210055149A1
公开(公告)日：2021-02-25
申请号：US16996992
申请日：2020-08-19
Applicant: Waters Technologies Corporation
Inventor： Jason F. Hill , Martin Gilar , Abhijit Tarafder , Christopher DesJardins
Abstract: Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.
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公开(公告)号：US09829470B2
公开(公告)日：2017-11-28
申请号：US14898272
申请日：2014-06-17
Applicant: Waters Technologies Corporation
Inventor： Geoff C. Gerhardt , Martin Gilar , Bernard Bunner
CPC classification number: G01N30/8668 , G01N30/6095 , G01N30/8665 , G01N2030/027
Abstract: Provided are systems and methods for adapting to volume variations in microfluidic chromatography columns. A column is calibrated by comparing a parameter of the column with a same parameter of a reference column and generating, by a processor, an adjustment factor in response to the comparison between the parameter of the column with a same parameter of the reference column. Volume differences between the calibrated column and the reference column are compensated for by integrating the generated adjustment factor into a sample separation involving the calibrated column.
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公开(公告)号：US20140065651A1
公开(公告)日：2014-03-06
申请号：US14075637
申请日：2013-11-08
Applicant: Waters Technologies Corporation
Inventor： Edouard S. Bouvier , Bruce J. Compton , John C. Gebler , Martin Gilar , Ying Q. Yu , Peter J. Lee , Elizabeth K. Brown
IPC: C12Q1/37
CPC classification number: C12Q1/37 , C12P21/06 , G01N27/44726 , G01N27/44747 , G01N33/5306
Abstract: The present invention provides methods for enhancing chemical reactions of molecules, e.g., biomolecules, with destructible surfactants. The chemical reactions may involve and/or be associate with analysis, e.g., solubilizing, separating, purifying and/or characterizing the molecules. In one aspect, the anionic surfactants of the present invention may be selectively broken up at relatively low pH. The resulting breakdown products of the surfactants may be removed from the molecule/sample with relative ease. The invention has applicability in a variety of analytical techniques.
Abstract translation: 本发明提供了增强具有可破坏表面活性剂的分子（例如生物分子）的化学反应的方法。 化学反应可能涉及和/或与分析有关，例如增溶，分离，纯化和/或表征分子。 在一个方面，本发明的阴离子表面活性剂可以在相对低的pH下选择性地分解。 可以相对容易地从分子/样品中除去所得表面活性剂的分解产物。 本发明适用于各种分析技术。
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Patent Agency Ranking
公开(公告)号：US20240302333A1
公开(公告)日：2024-09-12
申请号：US18645498
申请日：2024-04-25
Applicant: Waters Technologies Corporation
Inventor： Matthew A. Lauber , Martin Gilar , Mathew H. DeLano , Michael Donegan
CPC classification number: G01N30/26 , B01D15/1878 , B01D15/22 , B01D15/361 , B01D15/3847 , G01N2030/027
Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.
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公开(公告)号：US11994499B2
公开(公告)日：2024-05-28
申请号：US16800454
申请日：2020-02-25
Applicant: Waters Technologies Corporation
Inventor： Matthew A. Lauber , Martin Gilar , Mathew H. DeLano , Michael Donegan
CPC classification number: G01N30/26 , B01D15/1878 , B01D15/22 , B01D15/361 , B01D15/3847 , G01N2030/027
Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.
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公开(公告)号：US20240043907A1
公开(公告)日：2024-02-08
申请号：US18366149
申请日：2023-08-07
Applicant: Waters Technologies Corporation
Inventor： Martin Gilar , Catalin Doneanu , Matthew A. Lauber , Mame Maissa Gaye
IPC: C12Q1/6806 , G16B30/00
CPC classification number: C12Q1/6806 , G16B30/00
Abstract: The present disclosure describes methods, kits, and systems for digesting polyribonucleotides. The method involves selectively forming oligonucleotide (e.g., DNA:RNA or RNA:RNA) duplexes with single-stranded target RNA and then using sequence-specific nucleases that only act on RNA within duplexes to selectively cleave the target RNA into smaller fragments. Additional sequence-specific ribonucleases may be used to provide additional cuts of the target RNA at predetermined sites. By forming duplexes to increase the availability of nucleases that may be applied to cleave the single-stranded target RNA and selectively control where the target RNA is cleaved, the target RNA may be digested into fragments within controllable size ranges that are optimal for polynucletide analysis, such as by liquid chromatography and mass spectrometry.
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公开(公告)号：US20210199630A1
公开(公告)日：2021-07-01
申请号：US17057242
申请日：2019-05-20
Applicant: Waters Technologies Corporation
Inventor： Martin Gilar , Thomas S. McDonald , Joseph D. Michienzi , Jeffrey Musacchio , Rachel Mumma , Keith Fadgen , Geoff C. Gerhardt
Abstract: Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge (201) comprising a barrel (204) and a column (205), and especially including reinforcement to the column permitting high-pressure separation.
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20.发明申请公开(公告)号：US20200158605A1
公开(公告)日：2020-05-21
申请号：US16684930
申请日：2019-11-15
Applicant: Waters Technologies Corporation
Inventor： Abhijit Tarafder , Sebastien Besner , Mark Basile , Martin Gilar
Abstract: Exemplary embodiments are directed to methods and systems for controlling fluid parameters within a detector. A first and second fluid pump manager control the flow of a first and second fluid to one or more heating/cooling devices, and a mixer receives and mixes the first and second fluids. An optical detector flow cell receives the fluid mixture from the mixer, and a pressure regulator controls the pressure at the optical detector flow cell. Thus, the composition, temperature, and pressure of a fluid mixture entering an optical detector flow cell can be controlled in real time.
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