公开(公告)号：US08258464B2

公开(公告)日：2012-09-04

申请号：US12785945

申请日：2010-05-24

Applicant: Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: H01J49/34 ,  H01J37/244

CPC classification number: H01J49/025 ,  H01J49/0027

Abstract: A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.

Abstract translation: 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。

公开(公告)号：US4426576A

公开(公告)日：1984-01-17

申请号：US300019

申请日：1981-09-08

Applicant: G. Samuel Hurst ,  Marvin G. Payne ,  Chung-Hsuan Chen ,  James E. Parks

Inventor： G. Samuel Hurst ,  Marvin G. Payne ,  Chung-Hsuan Chen ,  James E. Parks

IPC: H01J49/02 ,  H01J49/04 ,  H01J49/16 ,  H01J49/42 ,  B01D59/44

CPC classification number: H01J49/162 ,  H01J49/0422

Abstract: Apparatus and methods of operation are described for determining, with isotopic selectivity, the number of noble gas atoms in a sample. The analysis is conducted within an evacuated chamber which can be isolated by a valve from a vacuum pumping system capable of producing a pressure of 10.sup.-8 Torr. Provision is made to pass pulses of laser beams through the chamber, these pulses having wavelengths appropriate for the resonance ionization of atoms of the noble gas under analysis. A mass filter within the chamber selects ions of a specific isotope of the noble gas, and means are provided to accelerate these selected ions sufficiently for implantation into a target. Specific types of targets are discussed. An electron measuring device produces a signal relatable to the number of ions implanted into the target and thus to the number of atoms of the selected isotope of the noble gas removed from the gas sample. The measurement can be continued until a substantial fraction, or all, of the atoms in the sample have been counted.Furthermore, additional embodiments of the apparatus are described for bunching the atoms of a noble gas for more rapid analysis, and for changing the target for repetitive cycling of the gas in the chamber. The number of repetitions of the cyclic steps depend upon the concentration of the isotope of interest, the separative efficiency of the mass filter, etc. The cycles are continued until a desired selectivity is achieved. Also described are components and a method of operation for a pre-enrichment operation for use when an introduction of a total sample would elevate the pressure within the chamber to levels in excess of those for operation of the mass filter, specifically a quadrupole mass filter.Specific examples of three noble gas isotope analyses are described.

Abstract translation: 描述了操作的装置和方法，以同位素选择性来确定样品中惰性气体原子的数量。 该分析在真空室内进行，其可以通过阀从能够产生10-8托的压力的真空泵系统隔离。 提供了将激光束的脉冲通过腔室，这些脉冲具有适于分析的惰性气体的原子的共振电离的波长。 室内的质量过滤器选择惰性气体的特定同位素的离子，并且提供了用于将这些选定的离子充分加速以植入靶中的装置。 讨论具体类型的目标。 电子测量装置产生与植入靶中的离子数相关的信号，从而产生从气体样品中除去的惰性气体的选定同位素原子数。 可以继续测量，直到已经计数了样品中的大部分原子或全部原子。 此外，描述了该设备的附加实施例，用于聚合惰性气体的原子以进行更快速的分析，并且用于改变靶室以使腔室中的气体重复循环。 循环步骤的重复次数取决于所关心的同位素的浓度，质量过滤器的分离效率等。循环持续到达到期望的选择性。 还描述了当全部样品的引入将室内的压力升高到超过用于质量过滤器，特别是四极质量过滤器的操作的水平时，用于预浓缩操作的组件和操作方法。 描述了三种惰性气体同位素分析的具体实例。

公开(公告)号：US09459247B2

公开(公告)日：2016-10-04

申请号：US13075160

申请日：2011-03-29

Applicant: Huan-Chang Lin ,  Hsin-Hung Lin ,  Cai-Yu Kao ,  Alice L Yu ,  Wen-Ping Peng ,  Chung-Hsuan Chen

Inventor： Huan-Chang Lin ,  Hsin-Hung Lin ,  Cai-Yu Kao ,  Alice L Yu ,  Wen-Ping Peng ,  Chung-Hsuan Chen

IPC: A61K45/06 ,  C12Q1/70 ,  G01N33/50 ,  G01N33/569 ,  G01N33/68

CPC classification number: G01N33/5091 ,  G01N33/5008 ,  G01N33/569 ,  G01N33/6848

Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。

公开(公告)号：US09321818B2

公开(公告)日：2016-04-26

申请号：US12703102

申请日：2010-02-09

Applicant: Chung-Hsuan Chen ,  Wei-Chao Chang ,  Michael Hsiao ,  Ming-Shyan Huang ,  Chih-Jen Yang

Inventor： Chung-Hsuan Chen ,  Wei-Chao Chang ,  Michael Hsiao ,  Ming-Shyan Huang ,  Chih-Jen Yang

IPC: C12Q1/00 ,  C07K14/47 ,  A61K31/7088 ,  A61K33/24 ,  A61K38/04 ,  A61K45/06 ,  G01N33/574 ,  G01N30/72 ,  G01N33/68

CPC classification number: C07K14/4723 ,  A61K31/7088 ,  A61K33/24 ,  A61K38/04 ,  A61K45/06 ,  G01N30/72 ,  G01N33/57423 ,  G01N33/57488 ,  G01N33/6851 ,  A61K2300/00

Abstract: Methods for diagnosis of non-small cell lung cancers by detection of endogenous peptides in exhaled breath condensate (EBC) are provided. Diagnostic peptides derived from dermcidin (DC) are provided. A specific dermcidin-derived peptide E-R11, having the sequence ENAGEDPGLAR (SEQ ID NO:2), is provided. E-R11 peptide levels in EBC, as measured by mass spectrometry (MS), are highly diagnostic of non-small cell lung cancers. A method for inhibiting growth of lung cancer cells by inhibiting DCD expression by RNA interference also is provided.

Abstract translation: 提供了通过检测呼出气体浓缩物（EBC）中的内源肽来诊断非小细胞肺癌的方法。 提供衍生自皮肤霉素（DC）的诊断肽。 提供具有序列ENAGEDPGLAR（SEQ ID NO：2）的具体的皮肤霉素衍生肽E-R11。 通过质谱（MS）测量，EBC中的E-R11肽水平是非小细胞肺癌的高度诊断。 还提供了通过RNA干扰抑制DCD表达来抑制肺癌细胞生长的方法。

公开(公告)号：US08507846B2

公开(公告)日：2013-08-13

申请号：US13566352

申请日：2012-08-03

Applicant: Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: B01D59/44 ,  H01J49/00

CPC classification number: H01J49/4285 ,  H01J49/0031 ,  H01J49/424

Abstract: An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by step scanning the driving frequency in frequency increments over a bandwidth, wherein for each step a specific frequency is held for a fixed number of complete cycles, wherein each specific frequency is changed continuously to the frequency in the next step, and wherein each specific frequency in each step starts at phase zero position.

Abstract translation: 一种离子阱质谱仪和用于通过在带宽上以频率增量扫描驱动频率来获得离子质谱的方法，其中对于每个步骤，特定频率保持固定数量的完整循环，其中每个特定频率被改变 在下一步骤中连续地进入频率，并且其中每个步骤中的每个特定频率从相位零位置开始。

公开(公告)号：US20110236882A1

公开(公告)日：2011-09-29

申请号：US13075160

申请日：2011-03-29

Applicant: Huan-Chang LIN ,  Hsin-Hung LIN ,  Cai-Yu KAO ,  Alice L. Yu ,  Wen-Ping PENG ,  Chung-Hsuan Chen

Inventor： Huan-Chang LIN ,  Hsin-Hung LIN ,  Cai-Yu KAO ,  Alice L. Yu ,  Wen-Ping PENG ,  Chung-Hsuan Chen

IPC: C12Q1/70 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: G01N33/5091 ,  G01N33/5008 ,  G01N33/569 ,  G01N33/6848

Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。

公开(公告)号：US20060024815A1

公开(公告)日：2006-02-02

申请号：US10903569

申请日：2004-07-30

Applicant: Steve Allman ,  Hannah Farquar ,  Chung-Hsuan Chen

Inventor： Steve Allman ,  Hannah Farquar ,  Chung-Hsuan Chen

IPC: C12M1/34

CPC classification number: G01N21/6452 ,  G01N21/253 ,  G01N33/54373

Abstract: A high sensitivity array-based detection system includes a plurality of probes immobilized onto a plurality of locations provided on a surface of a patterned array, such as a microarray. At least one target selectively binds to at least one of the plurality of probes when disposed proximate thereto under appropriate conditions, such as temperature and pH. The target includes at least one bound particle. The bound particle includes a plurality of label molecules. Alternatively, the target can be immobilized to the surface of the array, such as from a sample suspected of including the target. An excitation light source provides excitation light incident to one or more locations on the patterned array. A photodetector detects emanated signals from the patterned array. The photodetector can be a commercially available digital camera.

Abstract translation: 基于高灵敏度的基于阵列的检测系统包括固定在设置在诸如微阵列的图案化阵列的表面上的多个位置上的多个探针。 当在适当的条件下，例如温度和pH值时，至少一个靶选择性地结合至多个探针中的至少一个。 目标包括至少一个结合粒子。 结合粒子包括多个标记分子。 或者，靶可以被固定到阵列的表面，例如从怀疑包括靶的样品中。 激发光源提供入射到图案化阵列上的一个或多个位置的激发光。 光电检测器检测来自图案化阵列的发射信号。 光电检测器可以是市售的数码相机。

公开(公告)号：US5293130A

公开(公告)日：1994-03-08

申请号：US724655

申请日：1991-07-02

Applicant: Steve L. Allman ,  Fang C. Chen ,  Chung-Hsuan Chen

Inventor： Steve L. Allman ,  Fang C. Chen ,  Chung-Hsuan Chen

IPC: G01N27/64 ,  G01N27/66 ,  H01J47/04

CPC classification number: G01N27/66

Abstract: Apparatus for detecting an electronegative species comprises an analysis chamber, an inlet communicating with the analysis chamber for admitting a sample containing the electronegative species and an ionizable component, a radioactive source within the analysis chamber for emitting radioactive energy for ionizing a component of the sample, a proportional electron detector within the analysis chamber for detecting electrons emitted from the ionized component, and a circuit for measuring the electrons and determining the presence of the electronegative species by detecting a reduction in the number of available electrons due to capture of electrons by the electronegative species.

Abstract translation: 用于检测电负性物质的装置包括分析室，与分析室连通的入口，用于允许含有电负性物质的样品和可离子化组分，分析室内的放射源，用于发射用于电离样品组分的放射能， 在分析室内用于检测从电离成分发射的电子的比例电子检测器，以及用于测量电子并通过检测电负性捕获电子的可用电子数的减少来确定电负性物质的存在的电路 种类。

公开(公告)号：US08153964B2

公开(公告)日：2012-04-10

申请号：US12457064

申请日：2009-05-29

Applicant: Chung-Hsuan Chen ,  Chen-I Wu

Inventor： Chung-Hsuan Chen ,  Chen-I Wu

IPC: H01J49/26

CPC classification number: H01J49/10 ,  H01J49/0454

Abstract: Methods and systems for ultrasound ionization mass spectrometry are provided. Analytes in a sample are ionized by subjecting them to ultrasound, facilitating their analysis by mass spectrometry. With these methods and systems, soft ionization of large analytes, including biological macromolecules and nanoparticles, can be achieved. Ionization efficiency can be improved by addition of chemicals such as, for example, organic solvents or acids to the sample.

Abstract translation: 提供了超声电离质谱法的方法和系统。 样品中的分析物通过对其进行超声波离子化，便于通过质谱法进行分析。 通过这些方法和系统，可以实现大分子的软电离，包括生物大分子和纳米颗粒。 通过向样品中加入化学物质，例如有机溶剂或酸，可以提高电离效率。

公开(公告)号：US20110284733A1

公开(公告)日：2011-11-24

申请号：US12785945

申请日：2010-05-24

Applicant: Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming Lee Chu

Inventor： Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming Lee Chu

IPC: H01J49/26 ,  B01D59/44

CPC classification number: H01J49/025 ,  H01J49/0027

Abstract: A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.

Abstract translation: 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。