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公开(公告)号：US06560859B1
公开(公告)日：2003-05-13
申请号：US09402890
申请日：1999-10-14
IPC分类号： G01N2726
CPC分类号： G01N27/44782 , Y10S83/95 , Y10T29/5148 , Y10T29/53391 , Y10T83/0596
摘要： Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.
摘要翻译： 毛细管柱（102）穿过并被插入橡胶板（14）中，橡胶板通过橡胶的弹力保持和固定，并且二维地布置在样品注射侧。 它通过将保持板（6a）从下方夹持而在安装在平面上的毛细管柱（102）上固定，并在检测侧从橡胶板（16）上方固定。 为了将毛细管柱（102）压靠在保持板6a上并将其固定在橡胶板（16）上，将橡胶板（16）固定在两侧的保持板（6a）上的保持板（6b） 提供毛细管柱（102）的布置。
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公开(公告)号：US06294337B1
公开(公告)日：2001-09-25
申请号：US09600747
申请日：2000-08-29
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6869 , C12Q2535/101 , C12Q2525/143 , C12Q2521/119 , C12Q2521/101
摘要： A method for sequencing a target DNA fragment in which along with amplification of the target DNA fragment, nucleic acid transcripts are generated using an RNA polymerase and the amplified target DNA fragments are used as templates in the presence of terminators for nucleic acid transcription reaction and the generated nucleic acid transcripts are analyzed, characterized in that the amplification of target DNA fragments and the generation of nucleic acid transcripts are carried out at a constant temperature is disclosed. The amplification of target DNA fragments and the generation of nucleic acid transcripts can be carried out around the room temperature. A DNA sequencing method using a novel method in which without using a thermo-resistant RNA polymerase, the amplification of target DNA fragments and generation of nucleic acid transcript can be carried out simultaneously in parallel is provided.
摘要翻译： 用于对靶DNA片段进行测序的方法，其中连同扩增靶DNA片段，使用RNA聚合酶产生核酸转录物，扩增的靶DNA片段用作核酸转录反应终止子存在下的模板， 分析了产生的核酸转录物，其特征在于公开了靶DNA片段的扩增和核酸转录物的产生在恒温下进行。 靶DNA片段的扩增和核酸转录物的产生可以在室温附近进行。 提供了使用其中不使用耐热RNA聚合酶的新方法的DNA测序方法，靶DNA片段的扩增和核酸转录物的产生可以并行同时进行。
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公开(公告)号：US6143528A
公开(公告)日：2000-11-07
申请号：US297383
申请日：1999-07-16
CPC分类号： C12N15/1093 , C12N15/1096
摘要： Disclose is a method for making full-length cDNA libraries, which is for making libraries of cDNAs having lengths corresponding to full lengths of mRNAs and comprises the following steps of; forming RNA-DNA hybrids by reverse transcription starting from primers using mRNAs as templates, chemically binding a tag molecule to a diol structure present in the 5' Cap (.sup.7Me G.sub.ppp N) site of a mRNA which is forming a RNA-DNA hybrid, and separating RNA-DNA hybrids carrying a DNA corresponding to a full-length mRNA from the RNA-DNA hybrids formed above by using a function of the tag molecule. The present method is a method for preparing full-length cDNA libraries utilizing a method for labeling the 5' Cap site more efficiently than protein enzyme reactions, which is avoidable a decrease of a full-length cDNA synthesis efficiency caused by cleavage of mRNA, and can synthesize a full-length cDNA more efficiently.
摘要翻译： PCT No.PCT / JP97 / 03992 Sec。 371日期：1999年7月16日 102（e）日期1999年7月16日PCT提交1997年10月31日PCT公布。 公开号WO98 / 20122 PCT 日期1998年5月14日摘要是制备全长cDNA文库的方法，其用于制备具有对应于全长mRNA长度的cDNA文库，并且包括以下步骤： 通过使用mRNA作为模板从引物开始通过逆转录形成RNA-DNA杂交体，将标签分子化学结合存在于形成RNA-DNA杂交体的mRNA的5'帽（7MeGpppN）位点中的二醇结构，并分离RNA DNA杂交体通过使用标签分子的功能从上述形成的RNA-DNA杂交体携带对应于全长mRNA的DNA。 本方法是利用一种比蛋白酶反应更有效地标记5'帽位点的方法来制备全长cDNA文库的方法，可以避免由于mRNA切割引起的全长cDNA合成效率的降低，以及 可以更有效地合成全长cDNA。
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公开(公告)号：US6093300A
公开(公告)日：2000-07-25
申请号：US37005
申请日：1998-03-09
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44743 , Y10S436/809
摘要： A base plate is made of an insulating material, and comprised of a flat surface and a connector part connected therewith. A plurality of wells are vertically and transversely arranged on the surface of the base plate at regular intervals respectively, and the respective wells are provided with individual electrode patterns reaching the connector part from bottoms thereof through the surface of the base plate. The connector part is connected to an external high-voltage application part.
摘要翻译： 基板由绝缘材料制成，并且由平坦表面和与其连接的连接器部件组成。 多个孔分别以规则的间隔垂直和横向地布置在基板的表面上，并且各孔具有从其底部通过基板的表面到达连接器部分的单独的电极图案。 连接器部分连接到外部高压施加部件。
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15.发明授权Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers 有权包含可变内聚部分的寡核苷酸接头和通过使用所述接头制备多核苷酸文库的方法公开(公告)号：US08809518B2
公开(公告)日：2014-08-19
申请号：US12897745
申请日：2010-10-04
CPC分类号： C12P19/34 , C12N15/1096 , C12N15/66 , C40B40/00
摘要： The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.
摘要翻译： 本发明涉及包含寡核苷酸固定部分和由式（N）n表示的寡核苷酸可变部分的接头或接头群体，其中N为A，C，G，T或U，或其衍生物，n为 本发明的接头多核苷酸或接头多聚核苷酸群体可以由所述连接体或接头群体和与所述接头结合的靶第一链多核苷酸构成。 本发明还包括制备所述接头或接头群体的方法，以及使用所述接头或接头群体制备接头 - 多核苷酸的方法。 本发明的接头或多核苷酸接头可用于制备cDNA文库的方法。
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16.发明申请OLIGONUCLEOTIDE LINKERS COMPRISING A VARIABLE COHESIVE PORTION AND METHOD FOR THE PREPARATION OF POLYNUCLEOTIDE LIBRARIES BY USING SAID LINKERS 有权包含可变接合部分的寡核苷酸连接体和通过使用连接器制备多核苷酸文库的方法公开(公告)号：US20120028313A1
公开(公告)日：2012-02-02
申请号：US12897745
申请日：2010-10-04
CPC分类号： C12P19/34 , C12N15/1096 , C12N15/66 , C40B40/00
摘要： The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.
摘要翻译： 本发明涉及包含寡核苷酸固定部分和由式（N）n表示的寡核苷酸可变部分的接头或接头群体，其中N为A，C，G，T或U，或其衍生物，n为 本发明的接头多核苷酸或接头多聚核苷酸群体可以由所述连接体或接头群体和与所述接头结合的靶第一链多核苷酸构成。 本发明还包括制备所述接头或接头群体的方法，以及使用所述接头或接头群体制备接头 - 多核苷酸的方法。 本发明的接头或多核苷酸接头可用于制备cDNA文库的方法。
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17.发明授权Primer, primer set, and nucleic acid amplification method and mutation detection method using the same 有权引物，引物组和核酸扩增方法及使用其的突变检测方法公开(公告)号：US08067162B2
公开(公告)日：2011-11-29
申请号：US12075198
申请日：2008-03-10
CPC分类号： C12Q1/6853 , C12Q2525/117 , C12Q2563/107 , C12Q2565/101
摘要： The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.
摘要翻译： 本发明提供了有效地检测例如核酸的双螺旋结构的引物。 引物是含有至少一个下式（16）表示的结构的标记核酸，其中B是具有核碱基骨架的原子团，E是具有脱氧核糖骨架的原子团，核糖骨架或衍生的结构 或者具有肽结构或拟肽结构的原子团，Z 11和Z 12各自为表现出荧光且彼此相同或不同的原子团。
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公开(公告)号：US20110059869A1
公开(公告)日：2011-03-10
申请号：US12919594
申请日：2009-02-27
CPC分类号： C12N9/96 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q2527/127 , C12Q2527/125 , C12Q2521/543
摘要： An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.
摘要翻译： 本发明的目的是提供一种增加对固定在载体上的目标物质的酶反应性的方法; 以及减少或抑制载体对酶促反应的抑制作用的方法。 上述目的通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组中选出的物质存在而增加对固定在载体上的目标物质的酶反应性的方法来实现。 以及通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组的物质存在的方式，减少或抑制固定有靶物质的载体对目标物质的酶反应性的抑制作用。
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公开(公告)号：US07803579B2
公开(公告)日：2010-09-28
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of the sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of the sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac′), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between the sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 该方法包括提供一种引物，其在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）和5'侧杂交的序列（Ac'） 序列（Ac'）与位于靶核酸序列上的序列（A）的5'侧的序列（B）的互补序列（Bc）杂交的序列（B'），其中{X（ Y-Y'）} / X在-1.00至1.00的范围内，其中X表示序列中的碱基数（Ac'），Y表示侧翼为序列（A）的区域中的碱基数， 和（B），Y'表示序列（Ac'）和（B'）之间的间隔序列中的碱基数（Y'可以为零）。
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公开(公告)号：US20100047862A1
公开(公告)日：2010-02-25
申请号：US12083695
申请日：2006-10-20
CPC分类号： C12N9/1276 , C12N9/1252
摘要： This invention provides a novel DNA polymerase obtained from Bacillus smithii JCM9076, which has novel features in terms of, for example, optimal reaction conditions (e.g., optimal temperature) and enzyme activity. More particularly, a novel DNA polymerase is a pol I type DNA polymerase, which is any of proteins (a) to (f) below and has DNA polymerase activity: (a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 7; (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 7 by deletion, substitution, or addition of one or several amino acid residues; (c) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 9; and (d) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 9 by deletion, substitution, or addition of one or several amino acid residues.
摘要翻译： 本发明提供了一种从芽孢杆菌JCM9076获得的新型DNA聚合酶，其在例如最佳反应条件（例如最佳温度）和酶活性方面具有新特征。 更具体地，新型DNA聚合酶是pol I型DNA聚合酶，其是以下蛋白质（a）至（f）中的任一种，并且具有DNA聚合酶活性：（a）包含SEQ ID NO所示的氨基酸序列的蛋白质 ：7; （b）通过缺失，取代或添加一个或几个氨基酸残基的由SEQ ID NO：7所示的氨基酸序列衍生的氨基酸序列组成的蛋白质; （c）由SEQ ID NO：9所示的氨基酸序列组成的蛋白质; 和（d）通过缺失，取代或添加一个或几个氨基酸残基，由SEQ ID NO：9所示的氨基酸序列衍生的氨基酸序列组成的蛋白质。
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