公开(公告)号：US10815269B2

公开(公告)日：2020-10-27

申请号：US16115756

申请日：2018-08-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Gustav Rodrigo ,  Bjorn Noren ,  Virendra Kumbhar

IPC: B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32 ,  B01J41/20 ,  C07K1/16 ,  G01N33/544 ,  C07K16/00 ,  C07K16/06 ,  C07K1/36

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

公开(公告)号：US10562007B2

公开(公告)日：2020-02-18

申请号：US12667873

申请日：2008-07-22

Applicant: Andreas Axen ,  Jan Bergstrom ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Nils Norrman

Inventor： Andreas Axen ,  Jan Bergstrom ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Nils Norrman

IPC: B01D15/36 ,  B01J20/289 ,  B01J20/32

Abstract: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

公开(公告)号：US20200023338A1

公开(公告)日：2020-01-23

申请号：US16337236

申请日：2017-09-27

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Ann Catharina Bergh ,  Tania Ahmad ,  Mattias Algotsson ,  Therese Graner

IPC: B01J20/289 ,  B01D15/38 ,  B01J20/28 ,  B01J20/32

Abstract: The present invention relates to a novel chromatography media, more closely a novel IMAC (Immobilized Metal Affinity Chromatography) media. The novel chromatography media comprises a pentaligand and provides high dynamic binding capacity as well as high purity of the sample proteins purified on the media of the invention.

公开(公告)号：US20190241608A1

公开(公告)日：2019-08-08

申请号：US16386628

申请日：2019-04-17

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/06 ,  C07K16/00

CPC classification number: C07K1/22 ,  C07K1/145 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/55

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

公开(公告)号：US10343142B2

公开(公告)日：2019-07-09

申请号：US16189894

申请日：2018-11-13

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: B01J20/24 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  B01J20/28 ,  B01J20/285 ,  B01J20/289 ,  C07K17/10

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US10261057B2

公开(公告)日：2019-04-16

申请号：US15329199

申请日：2015-07-06

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Klaus Gebauer ,  Mats Lundkvist ,  Bjorn Olovsson

IPC: G01N30/60 ,  G01N30/46 ,  G01N30/02 ,  G01N30/88 ,  B01D15/18

Abstract: The invention discloses a flow control block for a stack of chromatography column modules, as well as a stack of chromatography modules comprising at least one flow control block. The flow control block is in a first position or configuration capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in parallel and in a second position or configuration it is capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in series.

公开(公告)号：US20190038996A1

公开(公告)日：2019-02-07

申请号：US16154639

申请日：2018-10-08

Applicant: GE Healthcare BioProcess R&D AB

Inventor： KLAUS GEBAUER

IPC: B01D15/14 ,  G01N30/46 ,  B01D15/18 ,  G01N30/60 ,  B01D15/22 ,  G01N30/88

Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US10048235B2

公开(公告)日：2018-08-14

申请号：US15379447

申请日：2016-12-14

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  G01N30/38 ,  B01D15/22 ,  B01D15/18 ,  B01D15/26

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US20180154281A1

公开(公告)日：2018-06-07

申请号：US15882998

申请日：2018-01-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： CARINA ENGSTRAND ,  ANNIKA FORSS ,  GUNNAR GLAD ,  BO-LENNART JOHANSSON ,  HANS J JOHANSSON ,  JEAN-LUC MALOISEL

IPC: B01D15/38 ,  B01J20/286 ,  C07K16/00 ,  B01D15/32 ,  B01D15/36 ,  C07K16/06 ,  B01J20/32 ,  B01J41/20 ,  C07K1/16 ,  C07K1/36

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

公开(公告)号：US09958421B2

公开(公告)日：2018-05-01

申请号：US13518029

申请日：2010-12-20

Applicant: Klaus Gebauer ,  Elisabeth Hallgren ,  Raf Lemmens ,  Ola Lind ,  Bjorn Lundgren ,  Jamil Shanagar ,  Kajsa Stridsberg-Friden ,  Helena Widegren

Inventor： Klaus Gebauer ,  Elisabeth Hallgren ,  Raf Lemmens ,  Ola Lind ,  Bjorn Lundgren ,  Jamil Shanagar ,  Kajsa Stridsberg-Friden ,  Helena Widegren

IPC: G01N30/56 ,  B01D15/20 ,  G01N30/28 ,  B01J20/28 ,  B01D15/32 ,  B01J20/22 ,  B01D15/36 ,  B01J20/281 ,  B01J20/30 ,  B01D15/38 ,  B01J20/285

CPC classification number: G01N30/56 ,  B01D15/206 ,  B01D15/32 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/22 ,  B01J20/28 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/3092 ,  G01N30/28 ,  G01N30/482

Abstract: The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.